Dynamic encoding of temperature in the central circadian circuit coordinates physiological activities

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 2, 2024

Abstract The circadian clock regulates animal physiological activities. How temperature reorganizes circadian-dependent activities remains elusive. Here, using in-vivo two-photon imaging with the control device, we investigated response of Drosophila central circuit to variation and identified that DN1as serves as most sensitive temperature-sensing neurons. gate DN1a’s diurnal response. Trans-synaptic tracing, connectome analysis, functional data reveal bidirectionally targets two neuronal subsets: activity-related E cells sleep-promoting DN3s. Specifically, behavioral demonstrate DN1a-E cell modulates evening locomotion peak in cold temperature, while DN1a-DN3 controls warm temperature-induced nocturnal sleep reduction. Our findings systematically comprehensively illustrate how dynamically integrates light signals effectively coordinate wakefulness at different times day, shedding on conserved neural mechanisms underlying temperature-regulated physiology animals.

Language: Английский

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Transcriptomic DN3 clock neuron subtypes regulate Drosophila sleep

Science Advances, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 3, 2025

Circadian neurons within animal brains orchestrate myriad physiological processes and behaviors, but the contribution of these to regulation sleep is not well understood. To address this deficiency, we leveraged single-cell RNA sequencing generate a comprehensive census transcriptomic cell types Drosophila clock neurons. We focused principally on enigmatic DN3s, which constitute most fly brain were previously almost completely uncharacterized. These DN3s are organized into 12 clusters with unusual gene expression features compared more well-studied further show that uncharacterized DN3 subtypes promote through G protein–coupled receptor, TrissinR . Our findings indicate an intricate behavior by highlight their remarkable diversity in functional properties.

Language: Английский

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Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation

Published: Feb. 28, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Language: Английский

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A subset of DN1p neurons integrates thermosensory inputs to promote wakefulness via CNMa signaling

Current Biology, Journal Year: 2021, Volume and Issue: 31(10), P. 2075 - 2087.e6

Published: March 19, 2021

Language: Английский

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A connectome of theDrosophilacentral complex reveals network motifs suitable for flexible navigation and context-dependent action selection

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: Dec. 9, 2020

ABSTRACT Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron-microscopy-based connectome of Drosophila CX, including all its neurons circuits at synaptic resolution. identified new CX neuron types, novel sensory motor pathways, network motifs that likely extract fly’s head-direction, maintain it with attractor dynamics, combine other sensorimotor information perform vector-based navigational computations. also numerous pathways may facilitate selection CX-driven behavioral patterns by context internal state. The provides comprehensive blueprint necessary for detailed understanding underlying flexible navigation, state-dependent action selection.

Language: Английский

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