Emerging imaging methods to study whole-brain function in rodent models

Translational Psychiatry, Journal Year: 2021, Volume and Issue: 11(1)

Published: Sept. 4, 2021

Abstract In the past decade, idea that single populations of neurons support cognition and behavior has gradually given way to realization connectivity matters complex results from interactions between remote yet anatomically connected areas form specialized networks. parallel, innovation in brain imaging techniques led availability a broad set tools characterize functional organization However, each these poses significant technical challenges faces limitations, which require careful consideration their underlying anatomical, physiological, physical specificity. this review, we focus on emerging methods for measuring spontaneous or evoked activity brain. We discuss can measure large-scale (directly indirectly) with relatively high temporal resolution, milliseconds seconds. further designed studying mammalian preclinical models, specifically mice rats. This field seen great deal recent years, facilitated by concomitant gene-editing possibility more invasive recordings. review aims give an overview currently available outlook future developments. information is suitable educational purposes assisting scientists choosing appropriate method own research question.

Language: Английский

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Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging

Journal of Visualized Experiments, Journal Year: 2021, Volume and Issue: 168

Published: Feb. 24, 2021

Functional ultrasound (fUS) imaging is a novel brain modality that relies on the high-sensitivity measure of cerebral blood volume achieved by ultrafast doppler angiography. As perfusion strongly linked to local neuronal activity, this technique allows whole-brain 3D mapping task-induced regional activation as well resting-state functional connectivity, non-invasively, with unmatched spatio-temporal resolution and operational simplicity. In comparison fMRI (functional magnetic resonance imaging), main advantage fUS consists in enabling complete compatibility awake behaving animal experiments. Moreover, mice, most used preclinical model Neuroscience, remains technically challenging due small size difficulty maintain stable physiological conditions. Here we present simple, reliable robust protocol for anesthetized mice using an off-the-shelf commercial system motorized linear transducer, yielding significant cortical following sensory stimulation reproducible connectivity pattern network identification.

Language: Английский

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A functional ultrasound brain GPS for automatic vascular-based neuronavigation

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: July 26, 2021

Abstract Recent advances in ultrasound imaging triggered by transmission of ultrafast plane waves have rendered functional (fUS) a valuable neuroimaging modality capable mapping cerebral vascular networks, but also for the indirect capture neuronal activity with high sensitivity thanks to neurovascular coupling. However, expansion fUS is still limited difficulty identify structures during experiments based solely on Doppler images and shape vessels. In order tackle this challenge, study introduces brain positioning system (BPS), GPS brain. The BPS whole-brain neuronavigation on-the-fly automatic alignment ultrasensitive transcranial Power volumic common templates such as Allen Mouse Brain Common Coordinates Framework. This method relies online registration complex fingerprint studied animal pre-aligned reference atlas, thus allowing rapid matching identification structures. We quantified accuracy using super-resolution obtained at microscopic scale Ultrasound Localization Microscopy found error 44 µm 96 intra-animal inter-animal registration, respectively. proposed approach outperforms manual landmark recognition performed expert neuroscientists (inter-annotator errors 215 259 µm). Using coupled Atlas, we demonstrated capability position itself automatically over chosen anatomical obtain corresponding activation maps even oblique planes. Finally, show that can be used acquire estimate connectivity matrices automatically. allows navigation could become key asset ensure standardized protocols non-expert researchers.

Language: Английский

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Functional Ultrasound Neuroimaging

Annual Review of Neuroscience, Journal Year: 2022, Volume and Issue: 45(1), P. 491 - 513

Published: July 8, 2022

Functional ultrasound (fUS) is a neuroimaging method that uses to track changes in cerebral blood volume as an indirect readout of neuronal activity at high spatiotemporal resolution. fUS capable imaging head-fixed or freely behaving rodents and producing volumetric images the entire mouse brain. It has been applied many species, including primates humans. Now reaching maturity, it being adopted by neuroscience community. However, nature signal different implementations are not necessarily accessible nonspecialists. This review aims introduce these concepts all neuroscientists. We explain physical basis principles method, present state art its hardware implementation, give concrete examples current applications neuroscience. Finally, we suggest areas for improvement during next few years.

Language: Английский

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Co-variations of cerebral blood volume and single neurons discharge during resting state and visual cognitive tasks in non-human primates

Cell Reports, Journal Year: 2023, Volume and Issue: 42(4), P. 112369 - 112369

Published: April 1, 2023

To better understand how the brain allows primates to perform various sets of tasks, ability simultaneously record neural activity at multiple spatiotemporal scales is challenging but necessary. However, contribution single-unit activities (SUAs) neurovascular remains be fully understood. Here, we combine functional ultrasound imaging cerebral blood volume (CBV) and SUA recordings in visual fronto-medial cortices behaving macaques. We show that provides a significant estimate response below typical fMRI spatial resolution 2mm3. Furthermore, our results also SUAs CBV are statistically uncorrelated during resting state correlate tasks. These have important implications for interpreting findings while one constructs inferences or

Language: Английский

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