Pathway-, layer- and cell-type-specific thalamic input to mouse barrel cortex

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Dec. 20, 2019

Mouse primary somatosensory barrel cortex (wS1) processes whisker sensory information, receiving input from two distinct thalamic nuclei. The first-order ventral posterior medial (VPM) nucleus most densely innervates layer 4 (L4) barrels, whereas the higher-order (medial part, POm) L1 and L5A. We optogenetically stimulated VPM or POm axons, recorded evoked excitatory postsynaptic potentials (EPSPs) in different cell-types across cortical layers wS1. found that neurons parvalbumin-expressing inhibitory received largest EPSPs, dominated by to L4 In contrast, somatostatin-expressing very little either pathway any layer. Vasoactive intestinal peptide-expressing an intermediate level of with less apparent layer-specificity. Our data help understand how wS1 neocortical microcircuits might process integrate inputs.

Language: Английский

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Recurrent interactions in local cortical circuits

Nature, Journal Year: 2020, Volume and Issue: 579(7798), P. 256 - 259

Published: March 4, 2020

Language: Английский

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Neuronal Circuits in Barrel Cortex for Whisker Sensory Perception

Physiological Reviews, Journal Year: 2020, Volume and Issue: 101(1), P. 353 - 415

Published: Aug. 20, 2020

The array of whiskers on the snout provides rodents with tactile sensory information relating to size, shape and texture objects in their immediate environment. Rodents can use detect stimuli, distinguish textures, locate navigate. Important aspects whisker sensation are thought result from neuronal computations somatosensory cortex (wS1). Each is individually represented somatotopic map wS1 by an anatomical unit named a ‘barrel’ (hence also called barrel cortex). This allows precise investigation processing context well-defined map. Here, we first review signaling pathways wS1, then discuss current understanding various types excitatory inhibitory neurons present within wS1. Different classes cells be defined according anatomical, electrophysiological molecular features. synaptic connectivity local microcircuits, as well long-range interactions impact neuromodulators, beginning understood. Recent technological progress has allowed cell-type-specific related activity during whisker-related behaviors. An important goal for future research obtain causal mechanistic how selected processed specific synaptically connected networks signaled downstream brain areas, thus contributing sensory-guided decision-making.

Language: Английский

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Cell-type-specific nicotinic input disinhibits mouse barrel cortex during active sensing

Neuron, Journal Year: 2021, Volume and Issue: 109(5), P. 778 - 787.e3

Published: Jan. 19, 2021

Fast synaptic transmission relies upon the activation of ionotropic receptors by neurotransmitter release to evoke postsynaptic potentials. Glutamate and GABA play dominant roles in driving highly dynamic activity synaptically connected neuronal circuits, but for other neurotransmitters are also expressed neocortex, including nicotinic receptors, which non-selective cation channels gated acetylcholine. To study function non-glutamatergic excitation we used two-photon microscopy target whole-cell membrane potential recordings different types genetically defined neurons layer 2/3 primary somatosensory barrel cortex awake head-restrained mice combined with pharmacological optogenetic manipulations. Here, report a prominent input, selectively depolarizes subtype GABAergic neuron expressing vasoactive intestinal peptide leading disinhibition during active sensorimotor processing. Nicotinic sensing might contribute importantly integration top-down motor-related signals necessary tactile perception learning.

Language: Английский

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Dynamics of a disinhibitory prefrontal microcircuit in controlling social competition

Neuron, Journal Year: 2021, Volume and Issue: 110(3), P. 516 - 531.e6

Published: Nov. 17, 2021

Language: Английский

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Cell-type-specific recruitment of GABAergic interneurons in the primary somatosensory cortex by long-range inputs

Cell Reports, Journal Year: 2021, Volume and Issue: 34(8), P. 108774 - 108774

Published: Feb. 1, 2021

Extensive hierarchical yet highly reciprocal interactions among cortical areas are fundamental for information processing. However, connectivity rules governing the specificity of such corticocortical connections, and top-down feedback projections in particular, poorly understood. We analyze synaptic strength from functionally relevant brain to diverse neuronal types primary somatosensory cortex (S1). Long-range different preferentially engage specific sets GABAergic neurons S1. Projections other cortices strongly recruit parvalbumin (PV)-positive lead PV neuron-mediated feedforward inhibition pyramidal In contrast, inputs whisker-related motor biased vasoactive intestinal peptide (VIP)-positive potentially result VIP disinhibition. Regardless input areas, somatostatin-positive receive relatively weak long-range inputs. Computational analyses suggest that a characteristic combination IN S1 represents area.

Language: Английский

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Dense functional and molecular readout of a circuit hub in sensory cortex

Science, Journal Year: 2022, Volume and Issue: 375(6576)

Published: Jan. 6, 2022

Although single-cell transcriptomics of the neocortex has uncovered more than 300 putative cell types, whether this molecular classification predicts distinct functional roles is unclear. We combined two-photon calcium imaging with spatial to functionally and molecularly investigate cortical circuits. characterized behavior-related responses across major neuronal subclasses in layers 2 or 3 primary somatosensory cortex as mice performed a tactile working memory task. identified an excitatory intratelencephalic type, Baz1a, that exhibits high feature selectivity. Baz1a neurons homeostatically maintain stimulus responsiveness during altered experience show persistent enrichment subsets immediately early genes. Functional anatomical connectivity reveals residing upper portions preferentially innervate somatostatin-expressing inhibitory neurons. This motif defines circuit hub orchestrates local sensory processing superficial neocortex.

Language: Английский

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Cortical disinhibitory circuits: cell types, connectivity and function

Trends in Neurosciences, Journal Year: 2021, Volume and Issue: 44(8), P. 643 - 657

Published: May 15, 2021

Language: Английский

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Neural Algorithms and Circuits for Motor Planning

Annual Review of Neuroscience, Journal Year: 2022, Volume and Issue: 45(1), P. 249 - 271

Published: March 22, 2022

The brain plans and executes volitional movements. underlying patterns of neural population activity have been explored in the context movements eyes, limbs, tongue, head nonhuman primates rodents. How do networks neurons produce slow dynamics that prepare specific fast ultimately initiate these movements? Recent work exploits rapid calibrated perturbations to test dynamical systems models are capable producing observed activity. These joint experimental computational studies show cortical during motor planning reflect fixed points (attractors). Subcortical control signals reshape move attractors over multiple timescales, causing commitment actions transitions movement execution. Experiments rodents beginning reveal how algorithms implemented at level brain-wide circuits.

Language: Английский

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Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types

Science, Journal Year: 2022, Volume and Issue: 378(6619)

Published: Nov. 3, 2022

Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current lack requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green red sensors, Ace-mNeon2 VARNAM2, their reverse response-polarity variants pAce pAceR. Our enable 0.4- 1-kilohertz recordings from >50 spiking neurons per field of view awake mice ~30-minute continuous imaging flies. Using dual-polarity multiplexed imaging, we uncovered brain state-dependent antagonism neocortical somatostatin-expressing (SST+) vasoactive intestinal peptide-expressing (VIP+) interneurons contributions hippocampal potentials ensembles with distinct axonal projections. By combining three mutually compatible indicators, performed simultaneous triple-population These approaches will empower investigations dynamic interplay neuronal subclasses at single-spike resolution.

Language: Английский

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