Brain stimulation, Journal Year: 2025, Volume and Issue: unknown
Published: May 1, 2025
Language: Английский
eLife, Journal Year: 2025, Volume and Issue: 13
Published: May 2, 2025
Two-photon (2P) fluorescence imaging through gradient index (GRIN) lens-based endoscopes is fundamental to investigate the functional properties of neural populations in deep brain circuits. However, GRIN lenses have intrinsic optical aberrations, which severely degrade their performance. aberrations decrease signal-to-noise ratio (SNR) and spatial resolution signals, especially lateral portions field-of-view (FOV), leading restricted FOV smaller number recorded neurons. This relevant for several millimeters length, are needed reach deeper regions rodent brain. We previously demonstrated a novel method enlarge improve 2P microendoscopes based on length <4.1 mm (Antonini et al., 2020). developed were too short most ventral mouse In this study, we combined simulations with fabrication aspherical polymer microlenses three-dimensional (3D) microprinting correct long (length >6 mm) (diameter, 500 µm). Long corrected had improved resolution, enabling significantly enlarged FOVs. Moreover, using synthetic calcium data showed that aberration correction enabled detection cells higher SNR fluorescent signals decreased cross-contamination between Finally, applied perform large-scale high-precision recordings neurons olfactory cortex, region laying approximately 5 from surface, awake head-fixed mice. powerful new tools population unprecedented large high
Language: Английский
Citations
0
eLife, Journal Year: 2024, Volume and Issue: 13
Published: June 13, 2024
Learning alters cortical representations and improves perception. Apical tuft dendrites in layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons barrel cortex as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not repeated stimulus exposure, enhanced selectivity for equally, even though only one was associated with reward. Selective tufts emerged from initially unresponsive or low-selectivity populations. Animal movement choice did account changes selectivity. Enhanced persisted after rewards removed animals ceased performing the task. conclude that learning produces long-lasting realignment dendrite responses behaviorally relevant dimensions
Language: Английский
Citations
3
Human Brain Mapping, Journal Year: 2024, Volume and Issue: 45(12)
Published: Aug. 15, 2024
Abstract Subjective cognitive decline (SCD) is a high‐risk population in the preclinical stage of Alzheimer's disease (AD), and olfactory dysfunction risk factor for dementia progression. The present study aimed to explore patterns functional connectivity (FC) changes neural circuits during stimulation SCD subjects. A total 56 subjects normal controls (NCs) were included. All assessed with scale, an behavior test, task‐based magnetic resonance imaging scanning. FC differences between two groups analyzed by generalized psychophysiological interaction. Additionally, we calculated compared activation brain regions within odor stimulation, volumetric showing groups, correlations neuroimaging indicators behavioral scale scores. During bilateral primary cortex (bPOC) right hippocampus group was significantly reduced; while frontal increased group. bPOC all showed significant activation, but no difference found. No observed volume or groups. positively correlated identification, functions. Furthermore, correlation threshold found whole cohort. These results suggested that structure remained stable, there (specifically, POC‐hippocampus‐frontal circuits) stimulation. findings highlight potential alterations as sensitive markers identifying individuals early AD.
Language: Английский
Citations
3
Frontiers in Aging Neuroscience, Journal Year: 2025, Volume and Issue: 17
Published: Feb. 20, 2025
Mild cognitive impairment (MCI) is associated with an increased risk of dementia in older adults. Olfactory may indicate prodromal dementia, yet its underlying mechanisms are not fully understood. This study aimed to investigate the alterations functional connectivity (FC) odor-induced olfactory neural circuits MCI patients. The included 39 patients and 42 normal controls (NCs). All subjects underwent assessments, behavior tests, odor-based magnetic resonance imaging (fMRI). Differences FC within were analyzed using generalized psychophysiological interaction (gPPI) method. showed significant deficits, including lower scores on Mini-Mental State Examination (MMSE) Montreal Cognitive Assessment (MoCA), alongside impairments episodic memory, visuospatial executive function, language, attention, threshold, total function. Compared NCs, exhibited reduced activation bilateral primary cortex (bPOC) during stimulation. Odor-induced bPOC correlated thresholds across cohort. During odor stimulation, from right anterior frontal lobe, particularly middle gyrus (MFG) superior (SFG). Conversely, lobe medial temporal cortex, fusiform parahippocampal gyri, was Increased SFG/MFG negatively while decreased fusiform/parahippocampal gyri positively language ability, identification. indicates that impaired (POC)-anterior cortex-medial circuit a sensitive neuroimaging marker for early dysfunction appears POC, suggesting this region provide novel diagnostic therapeutic avenues intervention.
Language: Английский
Citations
0
Brain stimulation, Journal Year: 2025, Volume and Issue: unknown
Published: May 1, 2025
Language: Английский
Citations
0