Excitatory Projections of Wide Field Collicular Neurons to the Nucleus of the Optic Tract in the Rat DOI Creative Commons
Αθανασία Τζάνου, Eirini Theodorou, Ioannis Mantas

et al.

The Journal of Comparative Neurology, Journal Year: 2024, Volume and Issue: 532(7)

Published: July 1, 2024

ABSTRACT The superficial layers of the mammalian superior colliculus (SC) contain neurons that are generally responsive to visual stimuli but can differ considerably in morphology and response properties. To elucidate structure function these neurons, we combined extracellular recording juxtacellular labeling, detailed anatomical reconstruction, ultrastructural analysis synaptic contacts labeled using transmission electron microscopy. Our project different brainstem nuclei. Of particular importance fit morphological criteria wide field (WF) whose dendrites horizontally oriented. They display a rather characteristic axonal projection pattern nucleus optic tract (NOT); thus, call them collicular WF projecting NOT (SCWF ) neurons. We corroborated characterization this neuronal type as distinct class with help unsupervised hierarchical cluster analysis. data demonstrate SCWF establish excitatory connections their targets NOT. Although, rodents, literature about has focused on extensive lateral posterior thalamus, conduit for information reach association areas cortex, our suggest subclass may participate optokinetic nystagmus.

Language: Английский

Characterize neuronal responses to natural movies in the mouse superior colliculus DOI Creative Commons
Ya-tang Li

Frontiers in Cellular Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: March 11, 2025

While artificial stimuli have been widely used in visual neuroscience and significantly advanced our understanding of processing, they differ dramatically from the natural scenes that animals encounter wild. How are encoded superior colliculus (SC) how neuronal responses to related remain poorly understood. Here I applied two-photon calcium imaging record activity mouse superficial SC response movies. An unsupervised learning algorithm grouped recorded neurons into 16 clusters based on their patterns. Each cluster exhibited distinct temporal profiles, which arose differences both receptive field coverage encode local features. Interestingly, found a strong correlation between movies functional properties previously characterized using stimuli. This suggests maintains stable neural representation information is largely independent types Furthermore, varied with depth within across genetically defined types. These findings bridge gap stimuli, providing new insights processing SC.

Language: Английский

Citations

0
Preference-independent saliency map in the mouse superior colliculus DOI Creative Commons
Ruixiang Wu,

Jinhuai Xu,

Chunpeng Li

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: April 4, 2025

Detecting salient stimuli in a visual scene is crucial for animal survival, yet how the brain encodes saliency remains unclear. Here, using two-photon calcium imaging, we reveal preference-independent map superficial superior colliculus of awake mice. Salient evoke stronger responses than uniform both excitatory and inhibitory neurons, with similar encoding patterns across cell types. The strongest response occurs when stimulus centered within receptive field, contextual effects extending approximately 40°. Response amplitude scales strength but independent neurons' orientation or motion direction preferences. Notably, saliency-encoding neurons exhibit weak selectivity, indicating complementary relationship between feature maps. Importantly, this does not require cortical inputs. These findings provide insights into neural mechanisms underlying detection.

Language: Английский

Citations

0
Neural dynamics in superior colliculus of freely moving mice DOI Creative Commons
Shelby L. Sharp, Jhoseph Shin, Dylan M. Martins

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 19, 2025

Abstract Vision is an active process that depends on head and eye movements to explore the visual environment. Superior colliculus (SC) known for its role in generating these movements, as well processing information, but has not been studied extensively during free movement complex environments. To determine impact of vision, we recorded neural activity across depth SC while simultaneously recording position. We find superficial (sSC) neurons respond input following gaze-shifting saccadic whereas deep (dSC) themselves, demonstrated by their sustained response darkness. Additionally, motor responses dSC are more correlated rather than movements. Furthermore, compared sSC gaze shift primary cortex (V1), finding similarities key types, although temporal sequences shifts differ between regions. Our results demonstrate distinct differences V1 highlighting various roles plays vision. Highlights depths superior freely moving mice measuring Neurons mouse strongly shifts, layers. primarily a movement. generally represent movement, independent input. While share with there unique profiles suggest

Language: Английский

Citations

0
Multipotent progenitors instruct ontogeny of the superior colliculus DOI Creative Commons
Giselle Cheung, Florian M. Pauler, Peter Koppensteiner

et al.

Neuron, Journal Year: 2023, Volume and Issue: 112(2), P. 230 - 246.e11

Published: Dec. 13, 2023

The superior colliculus (SC) in the mammalian midbrain is essential for multisensory integration and composed of a rich diversity excitatory inhibitory neurons glia. However, developmental principles directing generation SC cell-type are not understood. Here, we pursued systematic cell lineage tracing silico vivo, preserving full spatial information, using genetic mosaic analysis with double markers (MADM)-based clonal single-cell sequencing (MADM-CloneSeq). clonally related lineages revealed that radial glial progenitors (RGPs) exceptionally multipotent. Individual resident RGPs have capacity to produce all neuron types, even at stage terminal division. While individual units show no pre-defined cellular composition, establishment appropriate relative proportions distinct neuronal types occurs PTEN-dependent manner. Collectively, our findings provide an inaugural framework single-RGP/-cell level ontogeny.

Language: Английский

Citations

10
Mapping the Retina onto the Brain DOI Open Access
Daniel Kerschensteiner, Marla B. Feller

Cold Spring Harbor Perspectives in Biology, Journal Year: 2023, Volume and Issue: 16(2), P. a041512 - a041512

Published: Dec. 5, 2023

Daniel Kerschensteiner1,2,3 and Marla B. Feller4,5 1Department of Ophthalmology Visual Sciences 2Department Neuroscience 3Department Biomedical Engineering, Washington University School Medicine, St. Louis, Missouri 63110, USA 4Department Molecular Cell Biology 5Helen Wills Institute, California, Berkeley, California 94720, Correspondence: mfeller{at}berkeley.edu; kerschensteinerd{at}wustl.edu

Language: Английский

Citations

8
Enhancement and contextual modulation of visuospatial processing by thalamocollicular projections from ventral lateral geniculate nucleus DOI Creative Commons
Zhong Li, Bo Peng, Junxiang Huang

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 10, 2023

Abstract In the mammalian visual system, ventral lateral geniculate nucleus (vLGN) of thalamus receives salient input from retina and sends prominent GABAergic axons to superior colliculus (SC). However, whether how vLGN contributes fundamental information processing remains largely unclear. Here, we report in mice that facilitates visually-guided approaching behavior mediated by SC enhances sensitivity object detection. This can be attributed extremely broad spatial integration neurons, as reflected their much lower preferred frequencies broader receptive fields than neurons. Through thalamocollicular projections, specifically exerts surround suppression visuospatial SC, leading a fine tuning preferences higher smaller objects context-dependent manner. Thus, an essential component central pathway, serves refine contextually modulate SC-mediated visuomotor behaviors via visually-driven long-range feedforward inhibition.

Language: Английский

Citations

7
Differential expression analysis identifies candidate synaptogenic molecules for wiring direction-selective circuits in the retina DOI
Joshua Tworig, Ryan D. Morrie, Karina Bistrong

et al.

Journal of Neuroscience, Journal Year: 2024, Volume and Issue: unknown, P. e1461232024 - e1461232024

Published: March 21, 2024

An organizational feature of neural circuits is the specificity synaptic connections. A striking example direction-selective (DS) circuit retina. There are multiple subtypes DS retinal ganglion cells (DSGCs) that prefer motion along one four preferred directions. This computation mediated by selective wiring a single inhibitory interneuron, starburst amacrine cell (SAC), with each DSGC subtype preferentially receiving input from subset SAC processes. We hypothesize molecular basis this in part unique expression profiles subtypes. To test this, we first performed paired recordings isolated mouse retinas both sexes to determine postnatal day 10 (P10) represents age at which asymmetric synapses form. Second, RNA sequencing and differential analysis on P10 ON-OFF DSGCs tuned for either nasal or ventral identified candidates may promote direction-specific wiring. then used conditional knock-out strategy role candidate, secreted organizer cerebellin-4 (Cbln4), development tuning. Using two-photon calcium imaging, observed small deficit directional tuning among ventral-preferring lacking Cbln4, though whole-cell voltage-clamp did not identify significant change inputs. suggests Cbln4 does function primarily via cell-autonomous mechanism instruct circuits. Nevertheless, our transcriptomic candidate factors gaining insights into mechanisms circuit.

Language: Английский

Citations

2
Feature-independent Encoding of Visual Salience in the Mouse Superior Colliculus DOI Creative Commons
WU Rui-xiang,

Jinhuai Xu,

Chunpeng Li

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 6, 2024

Abstract Detecting conspicuous stimuli in a visual scene is crucial for animal survival, yet it remains debated how the brain encodes saliency. Here we investigate saliency represented superficial superior colliculus (sSC) of awake mice using two-photon calcium imaging. We report on preference-independent map sSC. Specifically, salient evoke stronger responses both excitatory and inhibitory neurons compared to uniform stimuli, with similar encoding patterns observed neuron types. The largest response occurs when stimulus positioned at receptive field center, contextual effects extending ∼40° away from center. amplitude correlates well strength not influenced by orientation or motion direction preferences neurons. However, does depend specific features. Furthermore, involved exhibit weak selectivity, suggesting complementary relationship between feature map.

Language: Английский

Citations

1
The superior colliculus: New insights into an evolutionarily ancient structure DOI Creative Commons

Teresa Guillamón-Vivancos,

Fabrizio Favaloro,

Francesco Dori

et al.

Current Opinion in Neurobiology, Journal Year: 2024, Volume and Issue: 89, P. 102926 - 102926

Published: Oct. 9, 2024

Language: Английский

Citations

1
Molecular organization of autonomic, respiratory, and spinally-projecting neurons in the mouse ventrolateral medulla DOI
Dana C. Schwalbe, Daniel S. Stornetta,

Ruei‐Jen Abraham‐Fan

et al.

Journal of Neuroscience, Journal Year: 2024, Volume and Issue: 44(31), P. e2211232024 - e2211232024

Published: June 25, 2024

The ventrolateral medulla (VLM) is a crucial region in the brain for visceral and somatic control, serving as significant source of synaptic input to spinal cord. Experimental studies have shown that gene expression individual VLM neurons predictive their function. However, molecular cellular organization has remained uncertain. This study aimed create comprehensive dataset cells using single-cell RNA sequencing male female mice. was enriched with targeted spinally-projecting adrenergic/noradrenergic neurons. Based on differentially expressed genes, resulting 114,805 identifies 23 subtypes neurons, excluding those inferior olive, five astrocytes. Spinally-projecting were found be abundant seven which validated through situ hybridization. These included serotonergic expressing markers associated premotor ventromedial medulla. Further analysis identified nine six subtypes, respectively, within each class monoaminergic Marker genes identify neural network responsible breathing concentrated two delineated from other by excitatory inhibitory datasets are available public download user-friendly interface. Collectively, this provides fine-scale identification VLM, forming foundation better understanding VLM's role vital functions motor control.

Language: Английский

Citations

1