Regulation of CeA-Vme projection in masseter hyperactivity caused by restraint stress DOI Creative Commons
Ya-Juan Zhao, Jihua Chen, Yang Liu

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Nov. 21, 2024

The overactivity of the masticatory muscles (bruxism or teeth clenching) is associated with stress exposure, and often leading to consistent muscle pain. However, neural mechanism underlining it not fully understood. central amygdala (CeA), which linked stress-induced behaviors physical reactions, projects directly mesencephalic trigeminal nucleus (Vme), crucial for oral-motor coordination. Thus, we hypothesized that projections from CeA Vme could be anxiety jaw muscles. After establishing an animal model restraint stress, found chronic lead noticeable anxiety-related behavior, increased masseter activity, activation GABAergic neurons in CeA, opposite changes excitability multipolar interneurons pseudounipolar excitatory Vme. Subsequently, through utilization anterograde transsynaptic tracing conjunction immunofluorescence staining, discovered were mainly terminated on within Moreover, chemogenetically suppressing function effectively reduce levels reverse increase activity induced by stress. And, specifically inhibiting via optogenetics hyperactivity but anxiety. In conclusion, our findings indicate may play important role response

Language: Английский

A distinct cortical code for socially learned threat DOI
Shana Silverstein,

Ruairi O’Sullivan,

Olena Bukalo

et al.

Nature, Journal Year: 2024, Volume and Issue: 626(8001), P. 1066 - 1072

Published: Feb. 7, 2024

Language: Английский

Citations

16
Functional architecture of dopamine neurons driving fear extinction learning DOI Creative Commons

Ximena I. Salinas-Hernández,

Daphne Zafiri,

Torfi Sigurdsson

et al.

Neuron, Journal Year: 2023, Volume and Issue: 111(23), P. 3854 - 3870.e5

Published: Sept. 22, 2023

The ability to extinguish fear responses stimuli that no longer predict danger is critical for adaptive behavior and increases the likelihood of survival. During extinction, dopamine (DA) neurons signal absence expected aversive outcome, this extinction prediction error (EPE) crucial initiating driving learning. However, neural circuits underlying EPE have remained elusive. Here, we investigate input-output circuitry EPE-encoding DA in male mice. By employing projection-specific fiber photometry optogenetics, demonstrate these project a restricted subregion nucleus accumbens. Comprehensive anatomical analyses, as well chemogenetic manipulations combined with recordings biosensors, further uncover dorsal raphe one key input structure generating signal. Together, our results reveal first time functional architecture

Language: Английский

Citations

27
Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice DOI
Sylvie L. Lesuis,

Sungmo Park,

Aijm Van Der Hoorn

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

12
Endocannabinoids facilitate transitory reward engagement through retrograde gain control DOI Open Access
David J. Marcus,

Anthony E. English,

Ge Chun

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Neuromodulatory signaling is poised to serve as a neural mechanism for gain control, acting crucial tuning factor influence neuronal activity by dynamically shaping excitatory and inhibitory fast neurotransmission. The endocannabinoid (eCB) system, the most widely expressed neuromodulatory system in mammalian brain, known filter inputs through retrograde, pre-synaptic action. However, whether eCBs exert retrograde control ultimately facilitate reward-seeking behaviors freely moving mammals not established. Using suite of vivo physiological, imaging, genetic machine learning-based approaches, we report fundamental role controlling behavioral engagement behavior defined thalamo-striatal circuit.

Language: Английский

Citations

1
Discrete prefrontal neuronal circuits determine repeated stress-induced behavioral phenotypes in male mice DOI
Haiyan Li, Ayako Kawatake-Kuno, Hiromichi Inaba

et al.

Neuron, Journal Year: 2024, Volume and Issue: 112(5), P. 786 - 804.e8

Published: Jan. 15, 2024

Language: Английский

Citations

7
Preclinical Behavioral and Pharmacological Treatments for Enhancing Fear Extinction in Adolescence DOI Creative Commons

Emily K. Wall,

Elizabeth A Virakorn, Kathryn Baker

et al.

Neuroscience & Biobehavioral Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 106090 - 106090

Published: March 1, 2025

Adolescence is a window of vulnerability for the development anxiety disorders but also opportunity treatments to minimize long-term impact such disorders. Current first-line treatments, primarily exposure-based cognitive-behavioral therapy (CBT), have limited efficacy in adolescents. The urgent need more effective interventions underscored by frequent reports extinction impairments adolescents as well rising rates youth, particularly post-COVID-19. Preclinical research on learned fear may contribute developing better treatment approaches this age group. Unfortunately, still largely under-explored area. However, both pharmacological and behavioral augmentation strategies can be used enhance learning consolidation. Here we describe work exploring adjuncts, focusing pre-clinical with rodents. Much date shows striking developmental differences response various only few shown Further, recent experience stress reduces these adolescence. This review highlights necessity tailored strategies, especially when it comes that address drug responses stressful experiences efficacy.

Language: Английский

Citations

0
Distinct neural responses of ventromedial prefrontal cortex-projecting nucleus reuniens neurons during aversive memory extinction DOI Creative Commons

Yasutaka Mochizuki,

Asuka Joji-Nishino,

Kazuo Emoto

et al.

Molecular Brain, Journal Year: 2025, Volume and Issue: 18(1)

Published: March 5, 2025

Animals adaptively regulate aversive memories in safe environments through extinction, a process central to exposure therapy for anxiety disorders. The limbic thalamus controls cognitive function concert with interconnected cortical and structures. Though medial prefrontal (mPFC) afferents the memory, functional role of efferents mPFC is unclear. Here, we investigated roles thalamic nuclei, reuniens (RE) mediodorsal (MD) thalamus, projecting cortex memory conditioning extinction male mice. Using retrograde tracing, demonstrated that ventromedial PFC (vmPFC)- dorsomedial (dmPFC)-projecting neurons are topologically segregated within RE MD. Fiber photometry revealed both RE→vmPFC MD→vmPFC respond stimuli. Notably, develop shock-associated cue (CS+) response during conditioning. During exhibited biphasic CS+, while showed no cue-evoked activity. Neither optogenetic activation nor inactivation these populations altered freezing behavior compared controls. Collectively, findings indicate encode information but dispensable behavioral modulation. This study highlights distinct contributions thalamus-PFC circuits processing.

Language: Английский

Citations

0
The CB1R of mPFC is involved in anxiety-like behavior induced by 0.8/2.65 GHz dual-frequency electromagnetic radiation DOI Creative Commons
Bin Sun,

Teng Xue,

Ai Gao

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 18

Published: March 12, 2025

As mobile phones and communication base stations become more widespread, concerns have arisen regarding the potential risks of environmental exposure to multi-frequency electromagnetic radiation (EMR) its effects on mental health. To address these concerns, our study established a dual-frequency EMR mouse model at 0.8/2.65 GHz explore molecular mechanisms intervention targets. Our results revealed that this significantly induced anxiety-like behavior in mice. Molecular experiments further showed significant decrease cannabinoid receptor type 1 (CB1R) levels medial prefrontal cortex (mPFC) mice, along with notable reduction endogenous cannabinoids 2-arachidonoylglycerol anandamide. This led downregulation entire endocannabinoid system (ECS). Additional confirmation was obtained by overexpressing knocking down CB1R mPFC. We found increasing mPFC could effectively reduce behavior, while decreasing exacerbated it. Furthermore, we change ECS basolateral amygdala (BLA). Notably, female mice exhibited similar behavioral phenotypes response EMR. In summary, demonstrates anxiety is closely linked function BLA, expression plays role modulating emotional

Language: Английский

Citations

0
Enhancing mPFC to BLA information transmission through chemical genetics to improve exploratory behavior in chronic stress rats DOI Creative Commons
Jin Guo,

Qingying Cao,

Huicong Jie

et al.

Brain Research Bulletin, Journal Year: 2025, Volume and Issue: 225, P. 111335 - 111335

Published: April 8, 2025

The pathogenesis of depression is fundamentally linked to the dysregulation neural circuit structure and function. Notably, medial prefrontal cortex (mPFC) basolateral amygdala (BLA) are critical brain regions in regulation depression-related behaviors. Depressed rats exhibited attenuated messaging between mPFC BLA, along with abnormally enhanced theta oscillations BLA during execution an exploratory task. However, whether specific activation improves behavior recovery mediated by mPFC-BLA circuitry unknown. We modeled using chronic unpredictable mild stimulation (CUMS) employed chemogenetic approaches selectively activate glutamatergic neurons depressed rats. Through simultaneous monitoring behavioral patterns local field potentials (LFPs) both open-field exploration, we conducted comparative analyses chemogenetically activated sham-stimulated groups. Our investigation focused on oscillation dynamics, network connectivity strength, interregional information transfer behavior. results demonstrated that not only ameliorated deficits but also mPFC-to-BLA while attenuating oscillations. These findings suggest restoration flow may play a crucial role improving behavior, thereby revealing potential mechanism underlying depressive state modulation.

Language: Английский

Citations

0
Stress-Induced Endogenous Cannabinoid Signaling Contributes to Fear Generalization DOI
Yanan Yue, Xia Zhang, Yuan Dong

et al.

Neuroscience Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Language: Английский

Citations

0