Activation of hypothalamic-pontine-spinal pathway promotes locomotor initiation and functional recovery after spinal cord injury in mice DOI Open Access

Chengyue Ji,

Y. Zhang,

Zeyu Lin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

SUMMARY The hypothalamus is critical for regulating behaviors essential survival and locomotion, but how it integrates internal needs transmits locomotion commands to the spinal cord (SC) remains unclear. We found that glutamatergic neurons in lateral hypothalamic area (LHA) are motivated locomotor activity. Using single-neuron projectome analysis, trans-synaptic tracing, optogenetic manipulation, we showed LHA facilitates during food seeking via pontine oral part (PnO) projection neurons, rather than direct SC projections or indirect stress signaling medial septum diagonal band. Activating PnO-SC also initiated locomotion. Importantly, LHA-PnO were crucial recovery following mouse injury (SCI). Closed-loop deep brain stimulation (DBS) of gating by motor cortex signals markedly promoted long-term restoration hindlimb functions after SCI. Thus, have identified a hypothalamic-pontine- pathway paradigm potential therapeutic intervention

Language: Английский

Optical sectioning methods in three-dimensional bioimaging DOI Creative Commons
Jing Zhang, Wei Qiao, Rui Jin

et al.

Light Science & Applications, Journal Year: 2025, Volume and Issue: 14(1)

Published: Jan. 1, 2025

Abstract In recent advancements in life sciences, optical microscopy has played a crucial role acquiring high-quality three-dimensional structural and functional information. However, the quality of 3D images is often compromised due to intense scattering effect biological tissues, compounded by several issues such as limited spatiotemporal resolution, low signal-to-noise ratio, inadequate depth penetration, high phototoxicity. Although various sectioning techniques have been developed address these challenges, each method adheres distinct imaging principles for specific applications. As result, effective selection suitable across diverse scenarios become yet challenging. This paper comprehensively overviews existing guidance under different scenarios. Specifically, we categorize microscope design based on spatial relationship between illumination detection axis, i.e., on-axis off-axis. classification provides unique perspective compare implementation performances approaches. Lastly, integrate selected methods custom-built off-axis system present future development techniques.

Language: Английский

Citations

0
Oxytocin improves maternal licking behavior deficits in autism-associated Shank3 mutant dogs DOI Creative Commons

Wen Lyu,

Yuan Li, Aiyu Yao

et al.

Translational Psychiatry, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 6, 2025

Impaired social interaction and repetitive behavior are key features observed in individuals with autism spectrum disorder (ASD). SHANK3 is a high-confidence ASD risk gene that encodes an abundant scaffolding protein the postsynaptic density. In wild-type (WT) domestic dogs, maternal behaviors such as licking nursing (largely milk feeding) of puppies most commonly observed. To address whether plays role especially behaviors, we analyzed Shank3 mutant dogs generated by CRISPR/Cas9 methodology. We found dams exhibited fewer shorter behavior, well reduced frequency when compared WT dams. Additionally, significant decrease blood oxytocin (OXT) concentration was detected thus conducted vehicle-controlled experiment to examine two-week intranasal OXT treatment, initiated on 8th postpartum day, could rescue deficits decreased significantly attenuated both acutely chronically treatment. The effect implicates oxytocinergic contribution defects dams, suggesting potential therapeutic strategy for SHANK3-associated ASD.

Language: Английский

Citations

0
The paraventricular nucleus of the hypothalamus: a key node in the control of behavioural states DOI Creative Commons
Karl J. Iremonger, Emmet M. Power

The Journal of Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

Abstract The paraventricular nucleus (PVN) of the hypothalamus contains diverse populations neuropeptide‐producing neurons. These include neurons that synthesise oxytocin, vasopressin, corticotropin‐releasing hormone, thyrotropin‐releasing hormone and somatostatin. While it is well established these control secretion neuroendocrine hormones, there growing evidence they also expression important homeostatic behaviours. Here we review recent data showing a critical role PVN in controlling arousal, social behaviour, defensive behaviour pain. Collectively, this suggests key node wider neural network behavioural states. image

Language: Английский

Citations

0
Activation of hypothalamic-pontine-spinal pathway promotes locomotor initiation and functional recovery after spinal cord injury in mice DOI Creative Commons
Yi Li,

Chengyue Ji,

Y. Zhang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Abstract The hypothalamus is critical for regulating behaviors essential survival and locomotion, but how it integrates internal needs transmits locomotion commands to the spinal cord (SC) remains unclear. We found that glutamatergic neurons in lateral hypothalamic area (LHA) are motivated locomotor activity. Using single-neuron projectome analysis, trans-synaptic tracing, optogenetic manipulation, we showed LHA facilitates during food seeking via pontine oral part (PnO) projection neurons, rather than direct SC projections or indirect stress signaling medial septum diagonal band. Activating PnO-SC also initiated locomotion. Importantly, LHA-PnO were crucial recovery following mouse injury (SCI). Motor cortex signals gated deep brain stimulation treatment markedly promoted long-term restoration of hindlimb motor functions after severe SCI. Thus, have identified a hypothalamic-pontine-spinal pathway paradigm potential therapeutic intervention

Language: Английский

Citations

0
Projectome-based characterization of hypothalamic peptidergic neurons in male mice DOI
Zhuo-Lei Jiao, Taosha Gao, Xiaofei Wang

et al.

Nature Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Language: Английский

Citations

0
Oxytocin and autism: Insights from clinical trials and animal models DOI
Chuan Xing, Xiang Yu

Current Opinion in Neurobiology, Journal Year: 2025, Volume and Issue: 92, P. 103015 - 103015

Published: March 30, 2025

Language: Английский

Citations

0
Lateralized local circuit tuning in female mouse auditory cortex DOI Creative Commons
Soomin C. Song, Robert C. Froemke

Neuroscience Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Evolution of Neuropeptide Signaling: From a Single Cell to Mammals DOI
Quirin Krabichler, Valery Grinevich

Masterclass in neuroendocrinology, Journal Year: 2025, Volume and Issue: unknown, P. 3 - 43

Published: Jan. 1, 2025

Language: Английский

Citations

0
Oxytocin‐Mediate Modulation of Splenic Immunosuppression in Chronic Social Stress Through Neuroendocrine Pathways DOI Creative Commons
Yi‐Shu Zhang, Haichao Chen, Jia‐Xin Cao

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Abstract Chronic social stress (CSS) is a significant public health challenge that negatively impacts behavior and immune function through brain‐spleen interactions. Oxytocin (OT), neuropeptide critical for regulation, upregulated during CSS, though its underlying mechanisms remain unclear. This study investigates the role of OT in splenic modulation using murine model CSS. Behavioral evaluations, serum oxytocin quantification, immunophenotypic analysis were performed. Splenic denervation confirmed OT’s neuromodulatory role, whereas OTR antagonism revealed endocrine function. CSS‐induced elevation was associated with immunosuppression, characterized by increased Foxp3⁺ regulatory T cells reduced CD4⁺ CD19⁺ B cells. also modulated macrophage polarization, inhibiting M1‐like (pro‐inflammatory) enhancing M2‐like (anti‐inflammatory) phenotypes. Denervation or pharmacological blockade signaling partly reversed immunosuppression but adversely affected survival CSS‐exposed mice. Additionally, mice's response to defeat, as shown decreased avoidance behavior. These findings suggest OT‐mediated likely represents compensatory mechanism chronic stress. Targeting OT–immune axis could offer innovative therapeutic approaches stress‐associated disorders restoring homeostasis while maintaining behavioral integrity.

Language: Английский

Citations

0
Oxytocin Improves Autistic Behaviors by Positively Shifting GABA Reversal Potential via NKCC1 in Early‐Postnatal‐Stage DOI Creative Commons

Zihui Wang,

Chang Xu,

YF Ma

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Accumulating evidence has identified disrupted oxytocin signaling in both autistic patients and animal models of autism. Nevertheless, the specific timing impact on social behavior remained unclear. Using mouse strains from oxytocin-Cre mice crossed with Cre-dependent chemogenetic mice, oxytocinergic neuronal activity is selectivity manipulated during early or late postnatal stages revealed, for first time, that suppression neurons rather than stage led to emergence autistic-like behaviors. Notably, significantly reduced levels are specifically valproic acid (VPA)-exposed Fmr1-KO brains, along an impairment GABA reversal potential downregulation Na+-K+-2Cl- cotransporter (NKCC1) post-birth. Furthermore, activation effectively restored aberrant NKCC1 expression GABAA receptor consequently alleviated behaviors VPA-exposed mice. Overall, results demonstrate may be unique critical period regulate promote brain development prosocial These findings suggest earlier intervention window strategy clinical treatment

Language: Английский

Citations

0