Activation of hypothalamic-pontine-spinal pathway promotes locomotor initiation and functional recovery after spinal cord injury in mice DOI Open Access

Chengyue Ji,

Y. Zhang,

Zeyu Lin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

SUMMARY The hypothalamus is critical for regulating behaviors essential survival and locomotion, but how it integrates internal needs transmits locomotion commands to the spinal cord (SC) remains unclear. We found that glutamatergic neurons in lateral hypothalamic area (LHA) are motivated locomotor activity. Using single-neuron projectome analysis, trans-synaptic tracing, optogenetic manipulation, we showed LHA facilitates during food seeking via pontine oral part (PnO) projection neurons, rather than direct SC projections or indirect stress signaling medial septum diagonal band. Activating PnO-SC also initiated locomotion. Importantly, LHA-PnO were crucial recovery following mouse injury (SCI). Closed-loop deep brain stimulation (DBS) of gating by motor cortex signals markedly promoted long-term restoration hindlimb functions after SCI. Thus, have identified a hypothalamic-pontine- pathway paradigm potential therapeutic intervention

Language: Английский

Differential Functions of Oxytocin Receptor-Expressing Neurons in the Ventromedial Hypothalamus in Social Stress Responses: Induction of Adaptive and Maladaptive Coping Behaviors DOI Creative Commons

Naranbat Nasanbuyan,

Masahide Yoshida, Ayumu Inutsuka

et al.

Biological Psychiatry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

2
Parallel Labeled-Line Organization of Sympathetic Outflow for Selective Organ Regulation in Mice DOI Creative Commons
Yukiko Harima, Masafumi Tsurutani,

Shuntaro Uchida

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 8, 2024

The sympathetic nervous system is vital in maintaining homeostasis and responding to environmental changes 1–3 . This regulation coordinated by the spinal preganglionic neurons (SPNs), which influence various organs both through neuronal pathways via postganglionic endocrine processes innervating adrenal gland. Despite decades of research supporting concept selective control within this 1,4–9 , neural circuit organization responsible for specificity outflow remains poorly understood. Notably, classical anatomical studies rats have not revealed a definitive molecular code governing SPNs, nor they confirmed existence SPNs strictly corresponding specific output targets 1,6,10,11 To reconcile discrepancy, we aim integrate recent transcriptome data 12,13 mice with viral-genetic toolkits 14 map axonal projections manipulate functions gastrointestinal tract Here, identified two subtypes lower thoracic cord, defined at level, exhibiting non-overlapping patterns innervation. Chemogenetic manipulations on these distinct SPN impacts digestive tracts or glucose metabolism mediated gland, respectively. molecularly delineated parallel labeled-line outflows presents potential avenue selectively manipulating organ functions.

Language: Английский

Citations

1
Neurobiological mechanisms underlying oxytocin-mediated parental behavior in rodents DOI Creative Commons
Kengo Inada

Neuroscience Research, Journal Year: 2024, Volume and Issue: 207, P. 1 - 12

Published: April 18, 2024

Parental behavior is essential for mammalian offspring to survive. Because of this significance, elucidating the neurobiological mechanisms that facilitate parental has received strong interest. Decades studies utilizing pharmacology and molecular biology have revealed in addition its facilitatory effects on parturition lactation, oxytocin (OT) promotes expression rodents. Recent also described modulation sensory processing by OT interaction system with other brain regions associated behavior. However, precise underlying facilitation caregiving behaviors remain unclear. In Review, I summarize findings from rats mice a view toward integrating past recent progress. then review advances understanding molecular, cellular, circuit OT-mediated Based these observations, propose hypothetical model would explain Finally, conclude discussing some major remaining questions potential future research directions.

Language: Английский

Citations

1
Spatiotemporal Mapping of the Oxytocin Receptor at Single-Cell Resolution in the Postnatally Developing Mouse Brain DOI Creative Commons
Hao Li, Ying Li, Ting Wang

et al.

Neuroscience Bulletin, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 15, 2024

Language: Английский

Citations

1
Oxytocin neurons signal state-dependent transitions to thermogenesis and behavioral arousal in social and non-social settings. DOI Open Access
Morgane Vandendoren,

Joseph F. Rogers,

Jason G. Landen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 22, 2024

Abstract Mammalian thermoregulatory behaviors such as thermal comfort seeking, physical activity, nesting, and huddling operate alongside autonomic responses brown fat thermogenesis peripheral vasodilation to defend core body temperature (Tb) 1–4 . The defended Tb is not held constant, but alternates across active/rest behavioral cycles 5–9 Although the facilitating these alternations are controlled by brain, underlying neural populations poorly understood. oxytocin system has been proposed contribute thermoregulation 10,11 , yet evidence for how activity within neurons relates pathways lacking. Here, we identify neuronal dynamics in mice. We show that paraventricular hypothalamus (PVN) PVN (PVN OT ) selectively activated during two states: active quiescent huddling. Next, activation inhibition of reveals effects on Tb, vasodilation, warm establishing a role thermoeffector pathways. then demonstrate vivo calcium tracks patterning behaviors. Across social contexts, peaks occur low (∼36.0°C) transitions towards thermogenesis. In solo context, predict offset quiescence onset post-quiescence nesting. paired post-quiescent Our study provides rest arousal foundation understanding coordination between homeostasis animal behavior. Graphical

Language: Английский

Citations

1
Parallel labeled-line organization of sympathetic outflow for selective organ regulation in mice DOI Creative Commons
Yukiko Harima, Masafumi Tsurutani, S. Yamada

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 10, 2024

The sympathetic nervous system is crucial for responding to environmental changes. This regulation coordinated by the spinal preganglionic neurons (SPNs), innervating both postganglionic and adrenal gland. Despite decades of research supporting concept selective control within this system, neural circuit organization responsible output specificity remains poorly understood. Here, combining recent single-cell transcriptome data with viral-genetic toolkits in mice, we identify two subtypes SPNs lower thoracic cord, defined at molecular level, exhibiting nonoverlapping patterns innervation: one specifically projecting celiac/superior mesenteric ganglia, other targeting grand. Chemogenetic manipulations on these distinct SPN revealed impacts motility gastrointestinal tracts or glucose metabolism mediated gland, respectively. molecularly delineated parallel labeled-line outflows presents a potential avenue selectively manipulating organ functions.

Language: Английский

Citations

1
The central oxytocinergic system of the prairie vole DOI Creative Commons
Eugénia Ramos,

G. M. Jiron,

Joshua S. Danoff

et al.

Brain Structure and Function, Journal Year: 2024, Volume and Issue: 229(7), P. 1737 - 1756

Published: July 23, 2024

Abstract Oxytocin (OXT) is a peptide hormone and neuropeptide that regulates various peripheral physiological processes modulates behavioral responses in the central nervous system. While humoral release occurs from axons arriving at median eminence, also released oxytocinergic cell brain structures contain its receptor, their dendrites hypothalamic nuclei potentially into cerebrospinal fluid (CSF). Understanding oxytocin’s complex functions requires knowledge on patterns of projections relationship to receptor (OXTR). This study provides first comprehensive examination system prairie vole ( Microtus ochrogaster ), an animal exhibiting social behaviors mirror human linked functioning. Using light electron microscopy, we characterized neuroanatomy this species. OXT+ bodies were found primarily hypothalamus, densest subcortical regions. Examination fibers oxytocin transcripts Oxtr ) revealed except for some structures, presence was not correlated with amount across brain. Of particular interest, cerebral cortex had high expression contained little no fibers. Electron microscopy used quantify dense cored vesicles (DCV) identify potential axonal sites. The ependymal cells line ventricles frequently permissive DCV-containing reaching third ventricle. Our results highlight mechanism which directly circulates throughout ventricular system, may serve as primary source binds OXTR cortex.

Language: Английский

Citations

0
Navigating Central Oxytocin Transport: Known Realms and Uncharted Territories DOI Creative Commons

Deniz Parmaksiz,

Yongsoo Kim

The Neuroscientist, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 7, 2024

Complex mechanisms govern the transport and action of oxytocin (Oxt), a neuropeptide hormone that mediates diverse physiologic processes. While Oxt exerts site-specific rapid effects in brain via axonal somatodendritic release, volume transmission CSF neurovascular interface can act as an additional mechanism to distribute signals across distant regions on slower timescale. This review focuses modes CNS, with particular emphasis roles perivascular spaces, blood-brain barrier (BBB), circumventricular organs coordinating triadic interaction among circulating blood, CSF, parenchyma. Perivascular critical conduits for flow, play pivotal role diffusion distribution within CNS reciprocally undergo Oxt-mediated structural functional reconstruction. BBB modulates movement between systemic cerebral circulation majority regions, without allow diffusion, monitoring, feedback regulation bloodborne peripheral such Oxt. Recognition these provides enhanced insight into propagation activity.

Language: Английский

Citations

0
Ginsenoside Rg1 promotes non-rapid eye movement sleep via inhibition of orexin neurons of the lateral hypothalamus and corticotropin-releasing hormone neurons of the paraventricular hypothalamic nucleus DOI Creative Commons
Yiyuan Wang, Yi‐Long Wu, Kewei Yu

et al.

Journal of Integrative Medicine, Journal Year: 2024, Volume and Issue: 22(6), P. 719 - 728

Published: Nov. 1, 2024

This study investigates the sleep-modulating effects of ginsenoside Rg1 (Rg1, C

Language: Английский

Citations

0
Activation of hypothalamic-pontine-spinal pathway promotes locomotor initiation and functional recovery after spinal cord injury in mice DOI Open Access

Chengyue Ji,

Y. Zhang,

Zeyu Lin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

SUMMARY The hypothalamus is critical for regulating behaviors essential survival and locomotion, but how it integrates internal needs transmits locomotion commands to the spinal cord (SC) remains unclear. We found that glutamatergic neurons in lateral hypothalamic area (LHA) are motivated locomotor activity. Using single-neuron projectome analysis, trans-synaptic tracing, optogenetic manipulation, we showed LHA facilitates during food seeking via pontine oral part (PnO) projection neurons, rather than direct SC projections or indirect stress signaling medial septum diagonal band. Activating PnO-SC also initiated locomotion. Importantly, LHA-PnO were crucial recovery following mouse injury (SCI). Closed-loop deep brain stimulation (DBS) of gating by motor cortex signals markedly promoted long-term restoration hindlimb functions after SCI. Thus, have identified a hypothalamic-pontine- pathway paradigm potential therapeutic intervention

Language: Английский

Citations

0