Anything but small: Microarousals stand at the crossroad between noradrenaline signaling and key sleep functions

Neuron, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Continuous sleep restores the brain and body, whereas fragmented harms cognition health. Microarousals (MAs), brief (3- to 15-s-long) wake intrusions into sleep, are clinical markers for various disorders. Recent rodent studies show that MAs during healthy non-rapid eye movement (NREM) driven by infraslow fluctuations of noradrenaline (NA) in coordination with electrophysiological rhythms, vasomotor activity, cerebral blood volume, glymphatic flow. hence part dynamics, raising questions about their biological roles. We propose bolster NREM sleep's benefits associated NA fluctuations, according an inverted U-shaped curve. Weakened noradrenergic as may occur neurodegenerative diseases or aids, reduce MAs, exacerbated caused stress fragment collapse signaling. suggest crucial restorative plasticity-promoting functions advance our insight normal pathological arousal dynamics from sleep.

Language: Английский

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A high-performance GRAB sensor reveals differences in the dynamics and molecular regulation between neuropeptide and neurotransmitter release

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 18, 2025

The co-existence and co-transmission of neuropeptides small molecule neurotransmitters within individual neuron represent a fundamental characteristic observed across various species. However, the differences regarding their in vivo spatiotemporal dynamics underlying molecular regulation remain poorly understood. Here, we develop GPCR-activation-based (GRAB) sensor for detecting short neuropeptide F (sNPF) with high sensitivity resolution. Furthermore, investigate between sNPF acetylcholine (ACh) from same neurons. Interestingly, our findings reveal distinct release ACh. Notably, results indicate that synaptotagmins (Syt) are involved these two processes, as Syt7 Sytα release, while Syt1 ACh release. Thus, this high-performance GRAB provides robust tool studying shedding insights into unique distinguish neurotransmitters.

Language: Английский

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Opioid use and abuse in adolescents and young adults; dealing with science, laws and ethics: Charming the COBRAS

Disease-a-Month, Journal Year: 2025, Volume and Issue: unknown, P. 101853 - 101853

Published: Jan. 1, 2025

Language: Английский

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Spatiotemporal Patterns Differentiate Hippocampal Sharp-Wave Ripples from Interictal Epileptiform Discharges in Mice and Humans

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract Hippocampal sharp-wave ripples (SPW-Rs) are high-frequency oscillations critical for memory consolidation in mammals. Despite extensive characterization rodents, their application as biomarkers to track and treat dysfunction humans is limited by coarse spatial sampling, interference from interictal epileptiform discharges (IEDs), lack of consensus on human SPW-R localization morphology. We demonstrate that mouse hippocampal share spatial, spectral temporal features, which clearly distinct IEDs. In 1024-channel recordings APP/PS1 mice, SPW-Rs were distinguishable IEDs narrow the CA1 pyramidal layer, narrowband frequency peaks, multiple ripple cycles unfiltered local field potential. epilepsy patients, showed similar peaks visible subiculum but absent dentate gyrus. Conversely, a broad extent wide-band power. introduce semi-automated, detection toolbox (“ripmap”) selecting optimal channels separating event waveforms low-dimensional embedding. Our approach improves accuracy, providing firm foundation future research.

Language: Английский

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Cholinergic dynamics in the septo-hippocampal system provide phasic multiplexed signals for spatial novelty and correlate with behavioral states

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

In the hippocampal formation, cholinergic modulation from medial septum/diagonal band of Broca (MSDB) is known to correlate with speed an animal's movements at sub-second timescales and also supports spatial memory formation. Yet, extent which dynamics, if all, align transient behavioral cognitive states supporting encoding novel information remains unknown. this study, we used fiber photometry record temporal dynamics in population activity septo-hippocampal neurons resolution during a hippocampus-dependent object location task using ChAT-Cre mice. Using general linear model, quantified were explained by changes movement speed, such as locomotion, grooming, rearing, recognizing familiar object. The data show that contain multiplexed code fast slow signals i) coding for logarithm timescales, ii) providing phasic novelty signal brief periods exploring location, iii) environmental seconds-long timescale. Furthermore, event-related around onset offset behavior demonstrates transients help facilitate switch state before behavior. These findings enhance understanding mechanisms contributes information.

Language: Английский

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Serotonin modulates infraslow oscillation in the dentate gyrus during Non-REM sleep

Published: Jan. 22, 2025

Synchronous neuronal activity is organized into oscillations with various frequency and time domains across different brain areas states. For example, hippocampal theta, gamma sharp wave are critical for memory formation communication between subareas the cortex. In this study, we investigated of dentate gyrus (DG) optical imaging tools during sleep-wake cycles. We found that major glutamatergic cell populations in DG infraslow (0.01 – 0.03 Hz) NREM sleep. Although considered a sparsely active network wakefulness, 50% granule cells about 25% mossy exhibit increased Further experiments revealed oscillation was correlated rhythmic serotonin release sleep, which oscillates at same but an opposite phase. Genetic manipulation 5-HT receptors neuromodulatory regulation mediated by 5-HT1a knockdown these leads to impairment. Together, our results provide novel mechanistic insights how system can influence patterns

Language: Английский

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Serotonin modulates infraslow oscillation in the dentate gyrus during Non-REM sleep

Published: March 14, 2025

Synchronous neuronal activity is organized into oscillations with various frequency and time domains across different brain areas states. For example, hippocampal theta, gamma sharp wave are critical for memory formation communication between subareas the cortex. In this study, we investigated of dentate gyrus (DG) optical imaging tools during sleep-wake cycles. We found that major glutamatergic cell populations in DG infraslow (0.01 – 0.03 Hz) NREM sleep. Although considered a sparsely active network wakefulness, 50% granule cells about 25% mossy exhibit increased sleep, compared to wakefulness. Further experiments revealed oscillation was correlated rhythmic serotonin release which oscillates at same but an opposite phase. Genetic manipulation 5-HT receptors neuromodulatory regulation mediated by 5-HT1a knockdown these leads impairment. Together, our results provide novel mechanistic insights how system can influence patterns

Language: Английский

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Serotonin modulates infraslow oscillation in the dentate gyrus during non-REM sleep

eLife, Journal Year: 2025, Volume and Issue: 13

Published: April 3, 2025

Synchronous neuronal activity is organized into oscillations with various frequency and time domains across different brain areas states. For example, hippocampal theta, gamma, sharp wave are critical for memory formation communication between subareas the cortex. In this study, we investigated of dentate gyrus (DG) optical imaging tools during sleep-wake cycles in mice. We found that major glutamatergic cell populations DG infraslow (0.01–0.03 Hz) NREM sleep. Although considered a sparsely active network wakefulness, 50% granule cells about 25% mossy exhibit increased sleep, compared to wakefulness. Further experiments revealed oscillation was correlated rhythmic serotonin release which oscillates at same but an opposite phase. Genetic manipulation 5-HT receptors neuromodulatory regulation mediated by Htr1a knockdown these leads impairment. Together, our results provide novel mechanistic insights how system can influence patterns

Language: Английский

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Monoaminergic signaling during mammalian NREM sleep - Recent insights and next-level questions

Current Opinion in Neurobiology, Journal Year: 2025, Volume and Issue: 92, P. 103025 - 103025

Published: April 22, 2025

Language: Английский

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Acetylcholine in the hippocampus: problems and achievements

Frontiers in Neural Circuits, Journal Year: 2025, Volume and Issue: 19

Published: April 30, 2025

Cholinergic septohippocampal projections originating from the medial septal area (MSA) play a critical role in regulating attention, memory formation, stress responses, and synaptic plasticity. axons MSA extensively innervate all hippocampal regions, providing structural basis for simultaneous release of acetylcholine (ACh) across entire hippocampus. However, this widespread appears inconsistent with specific functional roles that ACh is thought to serve during distinct behaviors. A key unresolved question how dynamics tissue concentrations determine its ability activate different receptor types coordinate individual pathways. Here, we highlight several debated issues, including potential intrinsic source within hippocampus – such as cholinergic interneurons co-release GABA. Furthermore, discuss recent findings on vivo concentration dynamics, which present new dilemma understanding signaling hippocampus: contrast between “global” release, driven by synchronous activation neurons, “local” may be influenced yet unidentified factors.

Language: Английский

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