Assembly of neuron- and radial glial cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons DOI Creative Commons

Ayumu Mubuchi,

Mina Takechi,

Shunsuke Nishio

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Nov. 9, 2023

Radial neuronal migration is a key neurodevelopmental event for proper cortical laminar organization. The multipolar-to-bipolar transition, critical step in establishing polarity during radial migration, occurs the subplate/intermediate zone (SP/IZ), distinct region of embryonic cerebral cortex. It has been known that extracellular matrix (ECM) molecules are enriched SP/IZ. However, molecular constitution and functions ECM formed this remain poorly understood. Here, we identified neurocan (NCAN) as major chondroitin sulfate proteoglycan mouse NCAN binds to both glial-cell-derived tenascin-C (TNC) hyaluronan (HA), large linear polysaccharide, forming ternary complex NCAN, TNC, HA Developing neurons make contact with migration. enzymatic or genetic disruption impairs by suppressing transition. Furthermore, TNC promoted morphological maturation vitro. present results provide evidence cooperative role neuron- development.

Language: Английский

MiRNA-501-3p and MiRNA-502-3p: A Promising Biomarker Panel for Alzheimer's Disease DOI Open Access
Davin Devara, Bhupender Sharma, Gunjan Goyal

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

ABSTRACT INTRODUCTION Alzheimer’s disease (AD) lacks a less invasive and early detectable biomarker. Here, we investigated the biomarker potential of miR-501-3p miR-502-3p using different AD sources. METHODS MiR-501-3p expressions were evaluated in CSF exosomes, serum familial sporadic fibroblasts B-lymphocytes by qRT-PCR analysis. Further, analyzed APP, Tau cells media exosomes. RESULTS significantly upregulated exosomes relative to controls. MiRNA levels high accordance with amyloid plaque NFT density multiple brain regions. Similarly, both miRNAs elevated MCI compared MiR-502-3p expression was fAD sAD B-lymphocytes. Finally, intracellularly secreted extracellularly response APP pathology. DISCUSSION These results suggest that could be promising biomarkers for AD.

Language: Английский

Citations

2
SynBot is an open-source image analysis software for automated quantification of synapses DOI Creative Commons
Justin T Savage, J.J. Ramirez, W. Christopher Risher

et al.

Cell Reports Methods, Journal Year: 2024, Volume and Issue: 4(9), P. 100861 - 100861

Published: Sept. 1, 2024

Language: Английский

Citations

6
SynBot: An open-source image analysis software for automated quantification of synapses DOI Open Access
Justin T Savage, J.J. Ramirez, W. Christopher Risher

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 28, 2023

The formation of precise numbers neuronal connections, known as synapses, is crucial for brain function. Therefore, synaptogenesis mechanisms have been one the main focuses neuroscience. Immunohistochemistry a common tool visualizing synapses. Thus, quantifying synapses from light microscopy images enables screening impacts experimental manipulations on synapse development. Despite its utility, this approach paired with low throughput analysis methods that are challenging to learn and results variable between experimenters, especially when analyzing noisy tissue. We developed an open-source ImageJ-based software, SynBot, address these technical bottlenecks by automating analysis. SynBot incorporates advanced algorithms ilastik SynQuant accurate thresholding synaptic puncta identification, code can easily be modified users. use software will allow rapid reproducible phenotypes in healthy diseased nervous systems.

Language: Английский

Citations

11
Extracellular Vesicle-Mediated Neuron–Glia Communications in the Central Nervous System DOI
Tsuneya Ikezu, Yongjie Yang, Claudia Verderio

et al.

Journal of Neuroscience, Journal Year: 2024, Volume and Issue: 44(40), P. e1170242024 - e1170242024

Published: Oct. 2, 2024

Communication between neurons and glia significantly influences the development maturation, plasticity, disease progressions of nervous system. As a new signaling modality, extracellular vesicles display diverse role for robust functional regulation through their protein nucleic acid cargoes. This review highlights recent breakthroughs in research mechanisms mediated by that are important neural development, axonal maintenance, synaptic functions, progression mammalian We will discuss biological roles released from neurons, astroglia, microglia, oligodendroglia system implications neurodegenerative disorders.

Language: Английский

Citations

4
Neuroplasticity in the transition from acute to chronic pain DOI Creative Commons

Qingbiao Song,

E Sihan,

Zhiyu Zhang

et al.

Neurotherapeutics, Journal Year: 2024, Volume and Issue: unknown, P. e00464 - e00464

Published: Oct. 1, 2024

Language: Английский

Citations

4
Neurocan regulates axon initial segment organization and neuronal activity DOI Creative Commons
David Baidoe‐Ansah, Hadi Mirzapourdelavar, Stepan Aleshin

et al.

Matrix Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Combined loss of brevican, neurocan, tenascin-C and tenascin-R leads to impaired fear retrieval due to perineuronal net loss DOI Creative Commons

Cornelius Mueller-Buehl,

Johanna Pakusch, Verian Bader

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 14, 2025

Abstract In conditions such as neurodegenerative diseases, posttraumatic stress disorder (PTSD), addiction and spinal cord injuries, restricted synaptic plasticity hinders the formation of new neuronal connections, preventing compensation treatment adverse behaviors. Perineuronal nets (PNNs) significantly restrict by inhibiting synapse formation. The digestion PNNs has been associated with short-term cognitive improvements reduced long-term memory, offering potential therapeutic benefits in PTSD. This study investigates correlation between fear memory processes extracellular matrix (ECM) mutant mice, particularly focusing on amygdala-medial prefrontal cortex (mPFC) circuit, which is crucial for generation maintenance. Fear conditioning was conducted mice lacking four key ECM-molecules: brevican, neurocan, tenascin-C tenascin-R (4x KO). These exhibited severe impairments consolidation, evident their inability to retrieve previously learned memories, coupled PNN density disturbed integrity along PNNs. Additionally, changes neural activity basolateral amygdala (BL) reductions VGAT + puncta amygdala-mPFC circuit were observed. contrast, tenascin single KOs showed intact behavior compared control groups. Impaired consolidation can be advantageous certain conditions, PTSD, making 4x KO an intriguing model future studies highlighting Tnc, Tnr compelling targets further investigation. underscores significance ECM regulation organization modulation a target memory-related conditions.

Language: Английский

Citations

0
Astrocyte-secreted factors modulate synaptic protein synthesis as revealed by puromycin labeling of isolated synaptosomes DOI Creative Commons
Aida de la Cruz, Jimena Baleriola

Frontiers in Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 18

Published: Feb. 20, 2025

The synaptic proteome can be shaped by proteins transported from the neuronal soma and/or mRNAs that are delivered to synapses where locally synthesized. This last mechanism is known as local translation. Local translation has been extensively studied in neurons physiological conditions and, more recently, neurological disorders, which transcriptomes and translatomes become dysregulated. It widely believed neurons, main source of localized transcripts primarily regulated neuron itself. However, we wondered whether glial cells, especially astrocytes, could contribute modulation protein synthesis. To address this question, compared levels produced compartments neuron-astrocyte co-cultures using modified Boyden chambers or astrocyte-conditioned medium. We developed a methodology measure synthesis puromycin labeling isolated synaptosomes devoid somatic input. Our results show enhanced retained when cultured presence astrocytes response Puromycin coupled with proximity ligation identified Rpl26 one whose astrocyte-secreted factors. thus unravel contribution glia point previously unexplored extra layer complexity regulation neurons.

Language: Английский

Citations

0
Neuroglia and extracellular matrix molecules DOI
Egor Dzyubenko, Dirk M. Hermann

Handbook of clinical neurology, Journal Year: 2025, Volume and Issue: unknown, P. 197 - 211

Published: Jan. 1, 2025

Language: Английский

Citations

0
Perineuronal Nets: Role in Normal Brain Physiology and Aging, and Pathology of various Diseases DOI
Kejian Zhu, Yifei Fu, Yan Zhao

et al.

Ageing Research Reviews, Journal Year: 2025, Volume and Issue: 108, P. 102756 - 102756

Published: April 18, 2025

Language: Английский

Citations

0