A Massively Parallel CRISPR-Based Screening Platform for Modifiers of Neuronal Activity DOI Creative Commons
Steven Boggess,

Vaidehi Gandhi,

Ming‐Chi Tsai

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 28, 2024

Understanding the complex interplay between gene expression and neuronal activity is crucial for unraveling molecular mechanisms underlying cognitive function neurological disorders. Here, we developed pooled screens activity, using CRISPR interference (CRISPRi) fluorescent calcium integrator CaMPARI2. Using this screening method, evaluated 1343 genes their effect on excitability in human iPSC-derived neurons, revealing potential links to neurodegenerative neurodevelopmental These include known regulators of excitability, such as TARPs ion channels, well associated with autism spectrum disorder Alzheimer's disease not previously described affect excitability. This CRISPRi-based platform offers a versatile tool uncover controlling health disease.

Language: Английский

A Massively Parallel CRISPR-Based Screening Platform for Modifiers of Neuronal Activity DOI Creative Commons
Steven Boggess,

Vaidehi Gandhi,

Ming‐Chi Tsai

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 28, 2024

Understanding the complex interplay between gene expression and neuronal activity is crucial for unraveling molecular mechanisms underlying cognitive function neurological disorders. Here, we developed pooled screens activity, using CRISPR interference (CRISPRi) fluorescent calcium integrator CaMPARI2. Using this screening method, evaluated 1343 genes their effect on excitability in human iPSC-derived neurons, revealing potential links to neurodegenerative neurodevelopmental These include known regulators of excitability, such as TARPs ion channels, well associated with autism spectrum disorder Alzheimer's disease not previously described affect excitability. This CRISPRi-based platform offers a versatile tool uncover controlling health disease.

Language: Английский

Citations

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