Trilobatin promotes angiogenesis after cerebral ischemia–reperfusion injury via SIRT7/VEGFA signaling pathway in rats DOI
Feng‐Ying Huang,

Lingyu Luo,

Yujia Wu

et al.

Phytotherapy Research, Journal Year: 2022, Volume and Issue: 36(7), P. 2940 - 2951

Published: May 10, 2022

Angiogenesis plays a pivotal role in the recovery of neurological function after ischemia stroke. Herein, we investigated effect trilobatin (TLB) on angiogenesis cerebral ischemia-reperfusion injury (CIRI). The TLB CIRI were mouse brain microvascular endothelium bEnd.3 cells and middle artery occlusion (MCAO)-induced rat model. cell proliferation observed using immunofluorescence staining. cycle, expressions cycle-related proteins SIRT 1-7 determined by flow cytometry western blot, respectively. binding affinity with SIRT7 was predicted molecular docking. results showed that concentration-dependently promoted proportion S-phase. significantly increased protein SIRT6, SIRT7, VEGFA, but not affected SIRT1-SIRT5 expressions. Moreover, only dramatically alleviated impairment CIRI, also enhanced post-stroke neovascularization newly formed functional vessels ischemic penumbra. Furthermore, up-regulated CDK4, cyclin D1, VEGFA its receptor VEGFR-2. Intriguingly, directly bound to expression at day 28. Our findings reveal promotes endothelial proliferation, facilitates via mediating SIRT7/VEGFA signaling pathway rats. Therefore, might be novel restorative agent rescue

Language: Английский

A Bibliometric Analysis of Pyroptosis From 2001 to 2021 DOI Creative Commons
Dan Ma, Bin Yang, Baoyi Guan

et al.

Frontiers in Immunology, Journal Year: 2021, Volume and Issue: 12

Published: Aug. 18, 2021

Pyroptosis is a new programmed cell death discovered in recent years. plays an important role various diseases. Nevertheless, there are few bibliometric analysis systematically studies this field. We aimed to visualize the research hotspots and trends of pyroptosis using help understand future development basic clinical research.The articles reviews regarding were culled from Web Science Core Collection. Countries, institutions, authors, references keywords field visually analyzed by CtieSpace VOSviewer software.A total 2845 included. The number significantly increased yearly. These publications mainly come 70 countries led China USA 418 institutions. identified 605 among which Thirumaladevi Kanneganti had most significant articles, Shi JJ was co-cited often. Frontiers immunology journal with studies, Nature commonly cited journal. After analysis, common nod like receptor family pyrin domain containing 3 inflammasome, apoptosis, death, gasdermin D, mechanism, caspase-1, others current developing areas study.Research on flourishing. Cooperation exchanges between institutions must be strengthened future. related pathway mechanism pyroptosis, relationship other types deaths as well diseases have been focus developmental research.

Language: Английский

Citations

173
Modulating the polarization phenotype of microglia – A valuable strategy for central nervous system diseases DOI
Yu Long,

Xiaoqiu Li,

Jie Deng

et al.

Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 93, P. 102160 - 102160

Published: Dec. 7, 2023

Language: Английский

Citations

42
Astragaloside IV Alleviates Cerebral Ischemia-Reperfusion Injury through NLRP3 Inflammasome-Mediated Pyroptosis Inhibition via Activating Nrf2 DOI Creative Commons
Lan Xiao, Ziwei Dai, Wenjing Tang

et al.

Oxidative Medicine and Cellular Longevity, Journal Year: 2021, Volume and Issue: 2021(1)

Published: Jan. 1, 2021

As one of the fundamental components Astragalus membranaceus, astragaloside IV (AST IV) exerts protective effects against cerebral ischemia-reperfusion injury (CIRI). Nevertheless, underlying mechanisms have not yet been conclusively elucidated. To do so, here, we report on regulatory Nrf2 NLRP3 inflammasome-mediated pyroptosis. CIRI was induced by middle artery occlusion-reperfusion (MCAO/R) in Sprague Dawley rats and modeled oxygen glucose deprivation/reoxygenation (OGD/R) SH-SY5Y cells. Cerebral infarct volume neurological deficit score served as indices to evaluate MCAO/R injury. In addition, CCK-8 assay used assess cell viability, LDH leakage rate a quantitative index, propidium iodide (PI) staining visualize cells after OGD/R The NLRP3/Caspase-1/GSDMD pathway, which produces pores membrane that are central pyroptosis process, assessed investigate activation detecting protein levels immunofluorescence analysis. We show rats, were strongly increased, cultures, viability decreased, proportion PI-positive increased. turn, enhanced NLRP3, Caspase-1, IL-1β, GSDMD, GSDMD-N. Moreover, translocation promoted CIRI. Interestingly, AST (i) reduced vivo (ii) increased vitro. GSDMD-N, inhibiting also transfer nucleus, accelerating activation. Particularly revealing inhibitor ML385 partly blocked above IV. Taken together, these results demonstrate alleviates through via activating Nrf2.

Language: Английский

Citations

92
Glial Cells as Therapeutic Approaches in Brain Ischemia-Reperfusion Injury DOI Creative Commons
Ivó H. Hernández, Mario Villa-González, Gerardo Martín-López

et al.

Cells, Journal Year: 2021, Volume and Issue: 10(7), P. 1639 - 1639

Published: June 30, 2021

Ischemic stroke is the second cause of mortality and first long-term disability constituting a serious socioeconomic burden worldwide. Approved treatments include thrombectomy rtPA intravenous administration, which, despite their efficacy in some cases, are not suitable for great proportion patients. Glial cell-related therapies progressively overcoming inefficient neuron-centered approaches preclinical phase. Exploiting ability microglia to naturally switch between detrimental protective phenotypes represents promising therapeutic treatment, similar way what happens with astrocytes. However, duality present many roles these cells upon ischemia poses notorious difficulty disentangling precise pathways target. Still, promoting M2/A2 microglia/astrocyte inhibiting M1/A1 neurotoxic profiles globally rendering results different vivo models stroke. On other hand, described oligodendrogenesis after brain seems be strictly beneficial, although less studied players paradigm negative effects could oligodendrocytes next years. Here, we review recent advances understanding role mentioned glial cell types main pathological events ischemic stroke, including inflammation, blood barrier integrity, excitotoxicity, reactive oxygen species management, metabolic support, neurogenesis, among others, special attention tested approaches.

Language: Английский

Citations

75
Microglia-mediated neuroinflammation and neuroplasticity after stroke DOI Creative Commons
Yuan Wang, Rehana K. Leak, Guodong Cao

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: Aug. 16, 2022

Stroke remains a major cause of long-term disability and mortality worldwide. The immune system plays an important role in determining the condition brain following stroke. As resident innate cells central nervous system, microglia are primary responders defense network covering entire parenchyma, exert various functions depending on dynamic communications with neurons, astrocytes, other neighboring under both physiological or pathological conditions. Microglia activation polarization is crucial for damage repair ischemic stroke, considered double-edged sword neurological recovery. can exist pro-inflammatory states promote secondary damage, but they also secrete anti-inflammatory cytokines neurotrophic factors facilitate recovery In this review, we focus mechanisms microglia-mediated neuroinflammation neuroplasticity after ischemia relevant potential microglia-based interventions stroke therapy.

Language: Английский

Citations

69
A comprehensive review on the neuroprotective potential of resveratrol in ischemic stroke DOI Creative Commons

Maryam Owjfard,

Zahra Rahimian, Farzaneh Karimi

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e34121 - e34121

Published: July 1, 2024

Stroke is the second leading cause of death and third disability worldwide. Globally, 68 % all strokes are ischemic, with 32 being hemorrhagic. Ischemic stroke (IS) poses significant challenges globally, necessitating development effective therapeutic strategies.IS among deadliest illnesses. Major functions played by neuroimmunity, inflammation, oxidative stress in multiple intricate pathways IS. Secondary brain damage specifically caused early pro-inflammatory activity that follows cerebral ischemia, which brought on excessive activation local microglia infiltration circulating monocytes macrophages.Resveratrol, a natural polyphenol found grapes berries, has shown promise as neuroprotective agent This review offers comprehensive overview resveratrol's role IS, focusing its mechanisms action potential. Resveratrol exerts effects activating nuclear factor erythroid 2-related 2 (NRF2) sirtuin 1 (SIRT1) pathways. SIRT1 resveratrol triggers deacetylation downstream targets like peroxisome proliferator-activated receptor-gamma coactivator alpha (PGC-1α) forkhead box protein O (FOXO), regulating mitochondrial biogenesis, antioxidant defense, cellular response. Consequently, promotes survival inhibits apoptosis IS.Moreover, activates NRF2 pathway, key mediator Activation through enhances expression enzymes, heme oxygenase-1 (HO-1) NAD(P)H quinone oxidoreductase (NQO1), neutralize reactive oxygen species mitigate ischemic brain. Combined, contributes to reducing stress, IS.Preclinical studies demonstrate improves functional outcomes, reduces infarct size, regulates blood flow preserves neuronal integrity. Gaining understanding these holds for targeted interventions aimed at promoting facilitating recovery IS patients aid future this matter.

Language: Английский

Citations

10
Molecular Mechanisms of Ischemic Stroke: A Review Integrating Clinical Imaging and Therapeutic Perspectives DOI Creative Commons

Sana Rehman,

Arsalan Nadeem, Umar Akram

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(4), P. 812 - 812

Published: April 7, 2024

Ischemic stroke poses a significant global health challenge, necessitating ongoing exploration of its pathophysiology and treatment strategies. This comprehensive review integrates various aspects ischemic research, emphasizing crucial mechanisms, therapeutic approaches, the role clinical imaging in disease management. It discusses multifaceted Netrin-1, highlighting potential promoting neurovascular repair mitigating post-stroke neurological decline. also examines impact blood–brain barrier permeability on outcomes explores alternative targets such as statins sphingosine-1-phosphate signaling. Neurocardiology investigations underscore contribution cardiac factors to mortality, importance understanding brain–heart axis for targeted interventions. Additionally, advocates early reperfusion neuroprotective agents counter-time-dependent excitotoxicity inflammation, aiming preserve tissue viability. Advanced techniques, including DWI, PI, MR angiography, are discussed their evaluating penumbra evolution guiding decisions. By integrating molecular insights with modalities, this interdisciplinary approach enhances our offers promising avenues future research interventions improve patient outcomes.

Language: Английский

Citations

9
Systemic immune responses after ischemic stroke: From the center to the periphery DOI Creative Commons
Fan Wu, Zongchi Liu, Lihui Zhou

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 20, 2022

Ischemic stroke is a leading cause of disability and death. It imposes heavy economic burden on individuals, families society. The mortality rate ischemic has decreased with the help thrombolytic drug therapy intravascular intervention. However, nerve damage caused by ischemia-reperfusion long-lasting followed multiple organ dysfunction. In this process, immune responses manifested systemic inflammatory play an important role. begins neuroinflammation following stroke. large number cells released after activation in lesion area, along deactivated neuroendocrine autonomic nervous systems, link center periphery. With immunity emergence immunosuppression, peripheral organs become second “battlefield” response gradually dysfunctional lead to adverse prognosis. purpose review was describe We hope provide new ideas for future research clinical treatments improve patient outcomes quality life.

Language: Английский

Citations

31
DTX3L induced NLRP3 ubiquitination inhibit R28 cell pyroptosis in OGD/R injury DOI Creative Commons

Ziyu Zhou,

Lei Shang, Qí Zhāng

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Journal Year: 2023, Volume and Issue: 1870(3), P. 119433 - 119433

Published: Jan. 25, 2023

Language: Английский

Citations

21
Glial Cells as Key Regulators in Neuroinflammatory Mechanisms Associated with Multiple Sclerosis DOI Open Access

Styliani Theophanous,

Irene Sargiannidou, Kleopas A. Kleopa

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9588 - 9588

Published: Sept. 4, 2024

Even though several highly effective treatments have been developed for multiple sclerosis (MS), the underlying pathological mechanisms and drivers of disease not fully elucidated. In recent years, there has a growing interest in studying neuroinflammation context glial cell involvement as is increasing evidence their central role progression. Although communication proper function underlies brain homeostasis maintenance, effects an MS remain complex controversial. this review, we aim to provide overview contribution cells, oligodendrocytes, astrocytes, microglia pathology during both activation orchestration inflammatory mechanisms, well synergistic repair restoration function. Additionally, discuss how understanding may new therapeutic targets either limit progression or facilitate repair.

Language: Английский

Citations

8