InSpectro-Gadget: A tool for estimating neurotransmitter and neuromodulator receptor distributions for MRS voxels DOI Creative Commons
Elizabeth McManus, Nils Muhlert, Niall W. Duncan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 5, 2023

Magnetic resonance spectroscopy (MRS) is widely used to estimate concentrations of glutamate and γ-aminobutyric acid (GABA) in specific regions the living human brain. As cytoarchitectural properties differ across brain, interpreting these measurements can be assisted by having knowledge such for MRS region(s) studied. In particular, some likely local neurotransmitter receptor patterns potentially give insights into mechanistic environment GABAand glutamatergic neurons are functioning in. This may particular utility when comparing two or more regions, given that populations substantially them. At same time, studying data from multiple participants timepoints, homogeneity sample becomes relevant, as taken areas with different cytoarchitecture difficult compare. To provide user-defined regions-of-interest, we produced an easy use tool InSpectro-Gadget, interfaces mRNA expression information Allen Human Brain Atlas. Python allows users input masks automatically obtain a graphical overview population found within. includes comparison between where relevant. The receptors subunit genes featured include GABA- classes, along wide range neuromodulators. functionality explained here its demonstrated through set example analyses. available at https://github.com/lizmcmanus/Inspectro-Gadget .

Language: Английский

Luminescent Lanthanide Infinite Coordination Polymers for Ratiometric Sensing Applications DOI Creative Commons
Zefeng Song, Yuanqiang Hao, Yunfei Long

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(2), P. 396 - 396

Published: Jan. 18, 2025

Ratiometric lanthanide coordination polymers (Ln-CPs) are advanced materials that combine the unique optical properties of ions (e.g., Eu3+, Tb3+, Ce3+) with structural flexibility and tunability polymers. These widely used in biological chemical sensing, environmental monitoring, medical diagnostics due to their narrow-band emission, long fluorescence lifetimes, excellent resistance photobleaching. This review focuses on composition, sensing mechanisms, applications ratiometric Ln-CPs. The mechanism relies two distinct emission bands, which provides a self-calibrating, reliable, precise method for detection. relative intensity ratio between these bands varies concentration target analyte, enabling real-time monitoring minimizing interference. approach is particularly suitable detecting trace analytes use complex environments where factors like background noise, temperature fluctuations, light variations may affect results. Finally, we outline future research directions improving design synthesis Ln-CPs, such as incorporating long-lifetime reference luminescent molecules, exploring near-infrared systems, developing up-conversion or two-photon materials. Progress areas could significantly broaden scope Ln-CP applications, especially biosensing, other fields.

Language: Английский

Citations

4
Additive Blending Effects on PEDOT:PSS Composite Films for Wearable Organic Electrochemical Transistors DOI Creative Commons

Hsueh-Sheng Tseng,

Ying-Lin Chen,

P. Zhang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(11), P. 13384 - 13398

Published: March 8, 2024

Organic electrochemical transistors (OECTs) employing conductive polymers (CPs) have gained remarkable prominence and undergone extensive advancements in wearable implantable bioelectronic applications recent years. Among the diverse arrays of CPs, poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) is a common choice for active-layer channel p-type OECTs, showing remarkably high transconductance amplification signals biosensing applications. This investigation focuses on novel engineering PEDOT:PSS composite materials by seamlessly integrating several additives, namely, dimethyl sulfoxide (DMSO), (3-glycidyloxypropyl)trimethoxysilane (GOPS), nonionic fluorosurfactant (NIFS), to fine-tune their electrical conductivity, self-healing capability, stretchability. To elucidate intricate influences DMSO, GOPS, NIFS additives formation films, theoretical calculations were performed, encompassing solubility parameters surface energies constituent components NIFS, PEDOT, PSS, PSS-GOPS polymers. Furthermore, we conducted comprehensive array material analyses, which reveal intricacies phase separation phenomenon its interaction with materials' characteristics. Our research identified optimal composition characterized outstanding stretchable capabilities. has proven be highly effective constructing an form OECT-based biosensors fabricated onto polydimethylsiloxane substrates detecting dopamine. Overall, these findings represent significant progress application films bioelectronics pave way development state-of-the-art technologies.

Language: Английский

Citations

14
Biosensors for psychiatric biomarkers in mental health monitoring DOI Creative Commons
Lin Wang, Yubing Hu, Nan Jiang

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 256, P. 116242 - 116242

Published: March 29, 2024

Psychiatric disorders are associated with serve disturbances in cognition, emotional control, or behavior regulation, yet few routine clinical tools available for the real-time evaluation and early-stage diagnosis of mental health. Abnormal levels biomarkers might imply biological, neurological, developmental dysfunctions psychiatric patients. Exploring biosensors that can provide rapid, in-situ, monitoring is therefore vital prevention, diagnosis, treatment, prognosis disorders. Recently, high sensitivity, selectivity, reproducibility have been widely developed, which mainly based on electrochemical optical sensing technologies. This review presented morbidity, disability, mortality, followed by describing pathophysiology a biomarker-implying manner. The latest developed detecting representative (e.g., cortisol, dopamine, serotonin) were comprehensively summarized compared their technologies, applicable biological platforms, integrative readouts. These well-developed promising facilitating utility commercialization point-of-care diagnostics. It anticipated healthcare be gradually improved multiple perspectives, from innovations terms biometric elements, transducing principles, flexible readouts, to construction "Big-Data" networks used share intractable indicators cases.

Language: Английский

Citations

13
Dysfunction of synaptic endocytic trafficking in Parkinson’s disease DOI Creative Commons
Xin Yi Ng, Mian Cao

Neural Regeneration Research, Journal Year: 2024, Volume and Issue: 19(12), P. 2649 - 2660

Published: March 1, 2024

Parkinson’s disease is characterized by the selective degeneration of dopamine neurons in nigrostriatal pathway and deficiency striatum. The precise reasons behind specific these remain largely elusive. Genetic investigations have identified over 20 causative PARK genes 90 genomic risk loci associated with both familial sporadic disease. Notably, several are linked to synaptic vesicle recycling process, particularly clathrin-mediated endocytosis pathway. This suggests that impaired might represent an early feature disease, followed axonal eventual loss cell bodies midbrain via a “dying back” mechanism. Recently, new animal cellular models disease-linked mutations affecting endocytic been created extensively characterized. These faithfully recapitulate certain disease-like features at animal, circuit, levels, exhibit defects membrane trafficking, further supporting findings from human genetics clinical studies. In this review, we will first summarize molecular two clathrin uncoating proteins: auxilin ( DNAJC6 / PARK19 ) synaptojanin 1 SYNJ1 PARK20 ). mouse carrying gene phenocopy each other terminal pathology display potent synergistic effect. Subsequently, delve into involvement endocytosis-related proteins (GAK, endophilin A1, SAC2/INPP5F, synaptotagmin-11), as factors through genome-wide association studies, pathogenesis. We also explore direct or indirect roles some common (alpha-synuclein (PARK1/4), Parkin (PARK2), LRRK2 (PARK8)) trafficking. Additionally, discuss emerging novel functions downstream traffic pathways, autophagy. Given dysfunction considered event deeper understanding mechanisms underlying trafficking may unveil targets for diagnosis development interventional therapies Future research should aim elucidate why generalized leads

Language: Английский

Citations

5
Enediol ligands-assembled nitrogen vacancies-g-C3N5@BiOBr for cathodic photoelectrochemical detection of dopamine DOI
Dandan Liu,

Longfei Zhu,

Chengxiang Li

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112688 - 112688

Published: Jan. 1, 2025

Language: Английский

Citations

0
Development of Fluorescence-Based Method for Dopamine Determination Using o-Phthaldialdehyde and 3-Mercaptopropyltriethoxysilane DOI Creative Commons
Valeriia V. Sliesarenko, Miloš Krstić,

Urban Bren

et al.

Sensors, Journal Year: 2025, Volume and Issue: 25(6), P. 1729 - 1729

Published: March 11, 2025

Nanomaterials and sensors play an important role in modern technologies, including medical diagnostics biochemical research. This work presents the possibility of using o-Phthaldialdehyde (OPA) combination with 3-mercaptopropyltriethoxysilane (MPTES) to develop a dopamine-responsive sensor. During experiment, these materials were used at different pH ratios determine optimal parameters for obtaining high fluorescence intensity reaction product. The data obtained demonstrate linear relationship between response (λex/λem = 340/460 nm) OPA/MPTES dopamine concentration range 0.1–3.0 µM 8, detection limit was 8.7 nM. results confirm potential as sensing component dopamine.

Language: Английский

Citations

0
In vivo multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors DOI
Yu Zheng, Ruyi Cai, Kui Wang

et al.

Science, Journal Year: 2025, Volume and Issue: 388(6751)

Published: June 5, 2025

Dopamine (DA) plays a crucial role in variety of brain functions through intricate interactions with other neuromodulators and intracellular signaling pathways. However, studying these complex networks has been hindered by the challenge detecting multiple neurochemicals vivo simultaneously. To overcome this limitation, we developed single-protein chemigenetic DA sensor, HaloDA1.0, which combines cpHaloTag–chemical dye approach G protein–coupled receptor activation–based (GRAB) strategy, providing high sensitivity for DA, subsecond response kinetics, far-red to near-infrared spectral range. When used together existing green red fluorescent neuromodulator sensors, calcium indicators, cyclic adenosine 5′-monophosphate optogenetic tools, HaloDA1.0 showed versatility multiplex imaging cultured neurons, slices, behaving animals, facilitating in-depth studies dynamic neurochemical networks.

Language: Английский

Citations

0
In vivo multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors DOI Creative Commons
Yu Zheng, Ruyi Cai, Kui Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Neurochemical signals like dopamine (DA) play a crucial role in variety of brain functions through intricate interactions with other neuromodulators and intracellular signaling pathways. However, studying these complex networks has been hindered by the challenge detecting multiple neurochemicals vivo simultaneously. To overcome this limitation, we developed single-protein chemigenetic DA sensor, HaloDA1.0, which combines cpHaloTag-chemical dye approach G protein-coupled receptor activation-based (GRAB) strategy, providing high sensitivity for DA, sub-second response kinetics, an extensive spectral range from far-red to near-infrared. When used together existing green red fluorescent neuromodulator sensors, Ca2+ indicators, cAMP optogenetic tools, HaloDA1.0 provides versatility multiplex imaging cultured neurons, slices, behaving animals, facilitating in-depth studies dynamic neurochemical networks.

Language: Английский

Citations

1
InSpectro-Gadget: A Tool for Estimating Neurotransmitter and Neuromodulator Receptor Distributions for MRS Voxels DOI
Elizabeth McManus, Nils Muhlert, Niall W. Duncan

et al.

Neuroinformatics, Journal Year: 2024, Volume and Issue: 22(2), P. 135 - 145

Published: Feb. 22, 2024

Language: Английский

Citations

0
Density functional theory analysis of iodine coadsorbed OH-copper phthalocyanine for dopamine sensing DOI
Thekkayil Sangeetha, Senthilkumar Lakshmipathi

Molecular Physics, Journal Year: 2024, Volume and Issue: 123(4)

Published: July 4, 2024

Density functional theory (DFT) was employed to investigate the sensing behaviour of neurotransmitter dopamine (DA) when interacting with OH-functionalised copper phthalocyanines (CuPcs) and coadsorbed iodine (CuIPc), both in gaseous aqueous media. This study revealed that CuIPc demonstrates a superior capacity for detecting molecules compared CuPc. Within these complexes, hydrogen bonds coordination were observed, playing pivotal role adsorption process. The enhanced electrical conductivity CuPc sheets after adsorption, along high energy iodine-coadsorbed CuPc/DA underscores significance this context. Notably, utilisation significantly enhances response dopamine. In summary, phthalocyanine has emerged as promising material sensors, offering possibilities further advancements field.

Language: Английский

Citations

0