Dopamine-mediated autocrine inhibition of insulin secretion DOI Creative Commons

Edoardo Ferrero,

Matilde Masini, Marco Carli

et al.

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 592, P. 112294 - 112294

Published: June 3, 2024

The aim of the present research was to explore mechanisms underlying role dopamine in regulation insulin secretion beta cells. effect on investigated INS 832/13 cell line upon glucose and other secretagogues stimulation. Results show that significantly inhibits stimulated by both secretagogues, while it has no basal secretion. This requires presence during incubation with various secretagogues. Both electron microscopy immunohistochemistry indicate cells D2 receptor is localized within granules. Blocking entry into granules inhibiting VMAT2 transporter tetrabenazine causes a significant increase ROS production. Our results confirm plays an important pancreatic through regulated precise compartmentalization mechanisms.

Language: Английский

Dopamine-mediated autocrine inhibition of insulin secretion DOI Creative Commons

Edoardo Ferrero,

Matilde Masini, Marco Carli

et al.

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 592, P. 112294 - 112294

Published: June 3, 2024

The aim of the present research was to explore mechanisms underlying role dopamine in regulation insulin secretion beta cells. effect on investigated INS 832/13 cell line upon glucose and other secretagogues stimulation. Results show that significantly inhibits stimulated by both secretagogues, while it has no basal secretion. This requires presence during incubation with various secretagogues. Both electron microscopy immunohistochemistry indicate cells D2 receptor is localized within granules. Blocking entry into granules inhibiting VMAT2 transporter tetrabenazine causes a significant increase ROS production. Our results confirm plays an important pancreatic through regulated precise compartmentalization mechanisms.

Language: Английский

Citations

1