Heat-inactivated Streptococcus pneumoniae augments circadian clock gene expression in zebrafish cells DOI Creative Commons
Camila Morales, Raina E. Sacksteder, Andrew G. Diamos

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 13, 2024

Abstract The circadian clock is a cell-autonomous process that regulates daily internal rhythms by interacting with environmental signals. Reports across species show infection can alter the expression of genes; however, in teleosts, these effects are influenced light exposure. Currently, no reports analyze direct bacterial exposure on zebrafish clock. Using Z3 cells, we demonstrate to heat-killed Streptococcus pneumoniae (HK-Spn) augments core repressive factors light- and time-dependent manner. In constant darkness, HK-Spn highly upregulated cry1a , per3 per1b expression. presence light, rapidly strongly per2 this was proportionally increased intensity. combinatorial effect not duplicated H 2 O 2, known byproduct However, ROS inhibitor N-acetyl cysteine sufficient block augmentation . These findings an inactive bacteria influences genes under different conditions.

Language: Английский

Stat3 Silencing Affects Circadian Clock Gene Expression and Lipopolysaccharide Response in the Suprachiasmatic Nucleus, Cortex, and Glioblastoma Cell Cultures DOI
Eva Filipovská, Kateřina Skálová, Veronika Spišská

et al.

The FASEB Journal, Journal Year: 2025, Volume and Issue: 39(10)

Published: May 12, 2025

ABSTRACT In mammals, the suprachiasmatic nucleus (SCN) serves as central circadian pacemaker, regulating rhythms essential for physiological processes. STAT3, a transcription factor primarily involved in immune signaling, exhibits rhythmicity SCN astrocytes. This study examined role of STAT3 regulation across several cell types, including primary cultures rat and cortex, cells organotypic slices from PER2::LUC mice, C6 glioblastoma cells. Furthermore, involvement inflammatory responses was investigated cortical cultures. silencing enhanced Bmal1 expression all tested disrupted cells, reduced amplitude PER2‐driven rhythm bioluminescence also attenuated its own Gfap , whereas it exhibited broader effects. Under LPS stimulation, most LPS‐induced genes, oxidative stress markers, while showing variable effects These findings indicate that clockwork appears consistent functional gene may vary depending on tissue differ between

Language: Английский

Citations

0
Heat-inactivated Streptococcus pneumoniae augments circadian clock gene expression in zebrafish cells DOI Creative Commons
Camila Morales, Raina E. Sacksteder, Andrew G. Diamos

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 13, 2024

Abstract The circadian clock is a cell-autonomous process that regulates daily internal rhythms by interacting with environmental signals. Reports across species show infection can alter the expression of genes; however, in teleosts, these effects are influenced light exposure. Currently, no reports analyze direct bacterial exposure on zebrafish clock. Using Z3 cells, we demonstrate to heat-killed Streptococcus pneumoniae (HK-Spn) augments core repressive factors light- and time-dependent manner. In constant darkness, HK-Spn highly upregulated cry1a , per3 per1b expression. presence light, rapidly strongly per2 this was proportionally increased intensity. combinatorial effect not duplicated H 2 O 2, known byproduct However, ROS inhibitor N-acetyl cysteine sufficient block augmentation . These findings an inactive bacteria influences genes under different conditions.

Language: Английский

Citations

0