Stat3 Silencing Affects Circadian Clock Gene Expression and Lipopolysaccharide Response in the Suprachiasmatic Nucleus, Cortex, and Glioblastoma Cell Cultures
The FASEB Journal,
Journal Year:
2025,
Volume and Issue:
39(10)
Published: May 12, 2025
ABSTRACT
In
mammals,
the
suprachiasmatic
nucleus
(SCN)
serves
as
central
circadian
pacemaker,
regulating
rhythms
essential
for
physiological
processes.
STAT3,
a
transcription
factor
primarily
involved
in
immune
signaling,
exhibits
rhythmicity
SCN
astrocytes.
This
study
examined
role
of
STAT3
regulation
across
several
cell
types,
including
primary
cultures
rat
and
cortex,
cells
organotypic
slices
from
PER2::LUC
mice,
C6
glioblastoma
cells.
Furthermore,
involvement
inflammatory
responses
was
investigated
cortical
cultures.
silencing
enhanced
Bmal1
expression
all
tested
disrupted
cells,
reduced
amplitude
PER2‐driven
rhythm
bioluminescence
also
attenuated
its
own
Gfap
,
whereas
it
exhibited
broader
effects.
Under
LPS
stimulation,
most
LPS‐induced
genes,
oxidative
stress
markers,
while
showing
variable
effects
These
findings
indicate
that
clockwork
appears
consistent
functional
gene
may
vary
depending
on
tissue
differ
between
Language: Английский
Heat-inactivated Streptococcus pneumoniae augments circadian clock gene expression in zebrafish cells
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 13, 2024
Abstract
The
circadian
clock
is
a
cell-autonomous
process
that
regulates
daily
internal
rhythms
by
interacting
with
environmental
signals.
Reports
across
species
show
infection
can
alter
the
expression
of
genes;
however,
in
teleosts,
these
effects
are
influenced
light
exposure.
Currently,
no
reports
analyze
direct
bacterial
exposure
on
zebrafish
clock.
Using
Z3
cells,
we
demonstrate
to
heat-killed
Streptococcus
pneumoniae
(HK-Spn)
augments
core
repressive
factors
light-
and
time-dependent
manner.
In
constant
darkness,
HK-Spn
highly
upregulated
cry1a
,
per3
per1b
expression.
presence
light,
rapidly
strongly
per2
this
was
proportionally
increased
intensity.
combinatorial
effect
not
duplicated
H
2
O
2,
known
byproduct
However,
ROS
inhibitor
N-acetyl
cysteine
sufficient
block
augmentation
.
These
findings
an
inactive
bacteria
influences
genes
under
different
conditions.
Language: Английский