Identification of putative baroreceptors in human aortic arch by histological and omics analyses DOI Creative Commons
Yankey Yundung, Jaroslav Pelisek,

Umberto Macciò

et al.

Hypertension Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Abstract Baroreflex regulates blood pressure and heartbeat through specific mechanosensitive baroreceptors. However, the current knowledge is derived only from animal experiments. No data about human aortic baroreceptors have been reported so far. Therefore, in this study, we performed extended histological, proteomics transcriptomics analyses of arch to identify potential Three healthy arches autopsies, six abdominal aneurysms four control tissue samples our vascular biobank were analysed. For histological analyses, antibodies against various neuronal markers used. Laser capture microdissection macrodissection selectively analyse nerves adventitia aorta using RNA sequencing. Histological analysis revealed a heterogeneous distribution along entire arch, predominantly ascending up left subclavian artery. Proteome identified three putative PIEZO1, TRPV2, TRPM4. Transcriptomics confirmed that these ion channels do not originate cells within wall but presumably cell body vagus nerve. Interestingly, also detected aneurysm without any significant differences their abundance. Our study identified, for first time, arch. Further studies are necessary validate results elucidate role

Language: Английский

Profiling local translatomes and RNA binding proteins of somatosensory neurons reveals specializations of individual axons DOI Open Access
Elizabeth S. Silagi,

E.U. Nduka,

Maria F. Pazyra‐Murphy

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 2, 2025

SUMMARY Individual neurons have one or more axons that often extend long distances and traverse multiple microenvironments. However, it is not known how the composition of individual established locally modulated to enable neuronal function plasticity. Here, we use spatial translatomics identify local axonal translatomes in anatomically functionally specialized dorsal root ganglia (DRG). DRG central peripheral opposite directions distinct microenvironments somatosensation. Using Translating Ribosome Affinity Purification RNA sequencing, generated a comprehensive resource mRNAs preferentially translated within each axon. Locally proteins include pain receptors, ion channels, translational machinery, which establish electrophysiologic properties regenerative capacities for We RNA-binding associated with sorting transporting related mRNAs. These findings provide resources addressing translation shapes organization enables subcellular neuroplasticity. HIGHLIGHTS Distinct are localized axons. Axonal govern capacity, electrophysiology. The RBP, SFPQ, coordinates mRNA towards somatosensory translatome data can be explored at painseq.shinyapps.io/CompartmentTRAP/.

Language: Английский

Citations

0
Identification of putative baroreceptors in human aortic arch by histological and omics analyses DOI Creative Commons
Yankey Yundung, Jaroslav Pelisek,

Umberto Macciò

et al.

Hypertension Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Abstract Baroreflex regulates blood pressure and heartbeat through specific mechanosensitive baroreceptors. However, the current knowledge is derived only from animal experiments. No data about human aortic baroreceptors have been reported so far. Therefore, in this study, we performed extended histological, proteomics transcriptomics analyses of arch to identify potential Three healthy arches autopsies, six abdominal aneurysms four control tissue samples our vascular biobank were analysed. For histological analyses, antibodies against various neuronal markers used. Laser capture microdissection macrodissection selectively analyse nerves adventitia aorta using RNA sequencing. Histological analysis revealed a heterogeneous distribution along entire arch, predominantly ascending up left subclavian artery. Proteome identified three putative PIEZO1, TRPV2, TRPM4. Transcriptomics confirmed that these ion channels do not originate cells within wall but presumably cell body vagus nerve. Interestingly, also detected aneurysm without any significant differences their abundance. Our study identified, for first time, arch. Further studies are necessary validate results elucidate role

Language: Английский

Citations

0