The effect of micro-vessel viscosity on the resonance response of a two-microbubble system DOI Creative Commons

Hossein Yusefi,

Brandon Helfield

Ultrasonics, Journal Year: 2024, Volume and Issue: 148, P. 107558 - 107558

Published: Dec. 18, 2024

Clinical ultrasound contrast agent microbubbles remain intravascular and are between 1-8 µm in diameter, with a volume-weighted mean size of 2-3 µm. Despite their worldwide clinical utility as diagnostic agent, continued ongoing success local therapeutic vector, the fundamental interplay - including bubble-bubble interaction effects neighboring viscoelastic vessel wall, poorly understood. In this work, we developed finite element model to study physics complex system two different-sized bubbles (2 3 diameter) confined within from resonance response perspective (3-12 MHz). Here, focus on effect micro-vessel wall viscosity resulting vibrational activity two-bubble system. The larger bubble (3 µm) was not influenced by its smaller companion bubble, observed significant dampening across all transmit frequencies when increasing viscosity, an expected result. However, highly induction strong low-frequency resonant frequency windows which lightly damped far exceeded vibration amplitude unconfined. Further, dynamics closely mimic frequency-dependence adjacent bubbles. Our findings imply that for multi-bubbles vessel, dominates inducing follow can be amplified vessel. These have important implications contrast-enhanced imaging applications.

Language: Английский

Focused Ultrasound and Microbubble-Mediated Delivery of CRISPR-Cas9 Ribonucleoprotein to Human Induced Pluripotent Stem Cells DOI

Kyle Hazel,

Davindra Singh,

Stephanie He

et al.

Molecular Therapy, Journal Year: 2025, Volume and Issue: 33(3), P. 986 - 996

Published: Jan. 10, 2025

Language: Английский

Citations

1
Shear Stress and Microbubble‐Mediated Modulation of Endothelial Cell Immunobiology DOI Creative Commons

Elahe Memari,

Davindra Singh,

Ryan Alkins

et al.

Small Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Cellular immunotherapy remains hindered in the context of solid tumors due to immunosuppressive microenvironment, which key endothelial cell adhesion molecules (CAM) are suppressed. Microbubble‐mediated focused ultrasound is being explored for targeted and can exert local shear stress upon neighboring cells. However, fluid microbubble‐induced modulation immunobiology not well understood. Herein, influence both types on human vein (HUVEC) brain (HBEC‐5i) CAM expression secretion over 90 cytokines using acoustically coupled microscopy examined. Fluid flow results time‐dependent expression, where ICAM‐1 peaked at 4 h (1.98‐fold, p < 0.001, HUVEC) 24 (1.56‐fold, HBEC‐5i). While some chemokines significantly enhanced (up 16.2‐fold; 0.001) from (e.g., IL‐8, MCP‐1, MCP‐3), others differentially expressed CCL5, CXCL‐16, SDF‐1). Under ultrasound, increased (≈1.4‐fold, 0.01) resulted significant large‐magnitude ( 0.05) differential 20 cytokines, most have immune‐activating function within a subset those induced by shear‐flow. regulates secretome toward an immune recruitment paradigm, thus may reinforce tumor cellular efforts.

Language: Английский

Citations

0
New insights into diabetes-induced cardiac pathology DOI
Kate L. Weeks, Bianca C. Bernardo, James R. Bell

et al.

Journal of Molecular and Cellular Cardiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
State of the art and perspectives of gene therapy in heart failure. A scientific statement of the Heart Failure Association of the ESC, the ESC Council on Cardiovascular Genomics and the ESC Working Group on Myocardial & Pericardial Diseases DOI Creative Commons
Sophie Van Linthout, Konstantinos Stellos, Mauro Giacca

et al.

European Journal of Heart Failure, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 22, 2024

Language: Английский

Citations

1
Flow rate modulates focused ultrasound-mediated vascular delivery of microRNA DOI Creative Commons
Stephanie He,

Davindra Singh,

Brandon Helfield

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2024, Volume and Issue: 36(1), P. 102426 - 102426

Published: Dec. 13, 2024

Gene therapy targeting ischemic heart disease is a promising therapeutic avenue, but it mostly restricted to viral-based delivery approaches which are limited due off-target immunological responses. Focused ultrasound presents non-viral, image-guided technique in circulating intravascular microbubble contrast agents can reversibly enhance vascular permeability and gene penetration. Here, we explore the influence of flow rate on microbubble-assisted miR-126, potent pro-angiogenic biologic, using custom acoustically coupled pressurized mesenteric artery model. We demonstrate that under same conditions, increased rates microbubble-mediated cell permeability; yet, miR-126 itself exhibits negative correlation with increasing velocity. Post-ultrasound assays confirmed vessel vasoreactivity, maintaining vasoconstriction vasodilation capacities. These findings underscore critical role plays focused therapy, especially notable for applications blood velocity salient pathophysiological indicator progression, including ischemia.

Language: Английский

Citations

0
The effect of micro-vessel viscosity on the resonance response of a two-microbubble system DOI Creative Commons

Hossein Yusefi,

Brandon Helfield

Ultrasonics, Journal Year: 2024, Volume and Issue: 148, P. 107558 - 107558

Published: Dec. 18, 2024

Clinical ultrasound contrast agent microbubbles remain intravascular and are between 1-8 µm in diameter, with a volume-weighted mean size of 2-3 µm. Despite their worldwide clinical utility as diagnostic agent, continued ongoing success local therapeutic vector, the fundamental interplay - including bubble-bubble interaction effects neighboring viscoelastic vessel wall, poorly understood. In this work, we developed finite element model to study physics complex system two different-sized bubbles (2 3 diameter) confined within from resonance response perspective (3-12 MHz). Here, focus on effect micro-vessel wall viscosity resulting vibrational activity two-bubble system. The larger bubble (3 µm) was not influenced by its smaller companion bubble, observed significant dampening across all transmit frequencies when increasing viscosity, an expected result. However, highly induction strong low-frequency resonant frequency windows which lightly damped far exceeded vibration amplitude unconfined. Further, dynamics closely mimic frequency-dependence adjacent bubbles. Our findings imply that for multi-bubbles vessel, dominates inducing follow can be amplified vessel. These have important implications contrast-enhanced imaging applications.

Language: Английский

Citations

0