Prediction of exosomal miRNA-based biomarkers for liquid biopsy DOI Creative Commons
Akanksha Arora, Gajendra P. S. Raghava

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 21, 2024

Abstract In this study, we investigated the properties of exosomal miRNAs to identify potential biomarkers for liquid biopsy. We collected 956 and non-exosomal miRNA sequences from RNALocate miRBase develop predictive models. Our initial analysis reveals that specific nucleotides are preferred at certain positions in associated with exosomes. employed an alignment-based approach, artificial intelligence (AI) models, ensemble methods predicting miRNAs. For used a motif-based method MERCI similarity-based BLAST, achieving high precision but low coverage about 29%. The AI developed using machine learning, deep learning techniques, large language achieved maximum AUC 0.707 MCC 0.268 on independent dataset. Finally, our method, combining AI-based reached 0.73 0.352 have web server, EmiRPred, assist scientific community designing identifying motifs ( https://webs.iiitd.edu.in/raghava/emirpred/ ). Key points Exosomal applications biopsy An has been predict design array models were built approaches (ML, DL, LLM) A variety important features identified webserver, python package, github, standalone software created

Language: Английский

Leveraging engineered yeast small extracellular vesicles serve as multifunctional platforms for effectively loading methyl salicylate through the “esterase-responsive active loading” strategy DOI
Tianhao Li, Yun Zhou, Haoran Wang

et al.

European Journal of Pharmaceutics and Biopharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 114696 - 114696

Published: March 1, 2025

Language: Английский

Citations

0
Extracellular vesicles in cancer´s communication: messages we can read and how to answer DOI Creative Commons
Alena Semerádtová, Michaela Liegertová, Regina Herma

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 19, 2025

Abstract Extracellular vesicles (EVs) are emerging as critical mediators of intercellular communication in the tumor microenvironment (TME), profoundly influencing cancer progression. These nano-sized vesicles, released by both and stromal cells, carry a diverse cargo proteins, nucleic acids, lipids, reflecting dynamic cellular landscape mediating intricate interactions between cells. This review provides comprehensive overview biogenesis, composition, functional roles EVs cancer, highlighting their significance basic research clinical applications. We discuss how cells manipulate EV biogenesis pathways to produce enriched with pro-tumorigenic molecules, explore specific contributions key hallmarks such angiogenesis, metastasis, immune evasion, emphasizing role shaping TME driving therapeutic resistance. Concurrently, we submit recent knowledge on can serve valuable source biomarkers for minimally invasive liquid biopsies, its potential, particularly targeted drug delivery vehicles immunomodulatory agents, showcasing promise enhancing efficacy safety treatments. By deciphering messages carried EVs, gain deeper understanding biology develop more effective strategies early detection, therapy, immunotherapy, paving way new era personalized precise medicine potential significantly improve patient outcomes.

Language: Английский

Citations

0
Comprehensive strategies for constructing efficient CRISPR/Cas based cancer therapy: Target gene selection, sgRNA optimization, delivery methods and evaluation DOI
Sathishbabu Paranthaman,

Chinnappa A. Uthaiah,

Shadab Md

et al.

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: unknown, P. 103497 - 103497

Published: March 1, 2025

Language: Английский

Citations

0
Novel Biological Strategies for Melanoma Therapy: A Focus on lncRNAs and Their Targeting DOI Open Access
Francesca Maria Orlandella, Rosaria Arcone, Neila Luciano

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(8), P. 1273 - 1273

Published: April 9, 2025

Increasing evidence revealed that restoring the correct expression of lncRNAs could have implications in management melanoma patients. In this context, here, we aim to dissect main characteristics altered and their crosstalk with signaling pathways involved progression disease. We also highlight role nucleic acid-based techniques natural compounds (i.e., phytochemicals) as a therapeutic tool increase or silence cancer cells. Finally, explore advances nanotechnologies delivery systems efficiently carry these chemicals into cells, thus limiting potential off-target effects. The analysis literature showed HOTAIR, MALAT1, H19 are oncogenic most studied melanoma, while MEG3 is an important tumor suppressor decreased cancer. aberrant affects several hallmarks cancer, e.g., proliferation, motility, epithelial mesenchymal transition, promoting plasticity drug resistance. frame, siRNA, antisense oligonucleotide, CRISPR-Cas9 genome editing appear be effective acid strategies restore physiologic lncRNA, curcumin, resveratrol, quercetin phytochemicals able target influence Overall, study provides comprehensive overview regarding phenotype cells targeting using RNA-based therapy products.

Language: Английский

Citations

0
Nano Approaches to Nucleic Acid Delivery: Barriers, Solutions, and Current Landscape DOI Creative Commons
J. González, Ki Wan Park, David Evans

et al.

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2025, Volume and Issue: 17(2)

Published: March 1, 2025

ABSTRACT Nucleic acid (NA) therapy holds tremendous potential for treating a wide range of genetic diseases by the delivery therapeutic genes into target cells. However, significant challenges exist in safely and effectively delivering these to their intended locations. Viral vectors, though efficient, pose risks such as immunogenicity mutagenesis. This has resulted growing interest non‐viral, nanoparticle‐based NA systems. review article describes various physiological barriers explores systems, including bioengineered nanoparticles, peptides, lipid polymeric highlighting unique features overcome vivo delivery. While systems offer promising alternative viral related cytotoxicity, reproducible synthesis, cost need be addressed. The current clinical landscape is also discussed, emphasizing safer, scalable, cost‐effective solutions. Nanoparticles represent future therapy, with possibility developing clinically relevant, non‐toxic, stable, non‐immunogenic vehicles, paving way broader applications improved outcomes.

Language: Английский

Citations

0
Genomic Modifications of Lactic Acid Bacteria and Their Applications in Dairy Fermentation DOI Creative Commons
Zifan Xie, Olivia McAuliffe, Yong‐Su Jin

et al.

Journal of Dairy Science, Journal Year: 2024, Volume and Issue: 107(11), P. 8749 - 8764

Published: July 3, 2024

Lactic Acid Bacteria (LAB) have a long history of safe use in milk fermentation and are generally recognized as health-promoting microorganisms when present fermented foods. LAB also important components the human intestinal microbiota widely used probiotics. Considering their health-beneficial properties, considered appropriate vehicles that can be genetically modified for food, industrial pharmaceutical applications. Here, this review describes (1) potential opportunities application strains dairy (2) various genomic modification tools strains, such random mutagenesis, adaptive laboratory evolution, conjugation, homologous recombination, recombineering, CRISPR (clustered regularly interspaced short palindromic repeat)- Cas (CRISPR-associated protein) based genome engineering. Lastly, discusses future developments these technologies applications fermentations.

Language: Английский

Citations

2
Basic Guide for Approaching Drug Delivery with Extracellular Vesicles DOI Open Access
Sergey Brezgin, Oleg Danilik, Alexandra Yudaeva

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10401 - 10401

Published: Sept. 27, 2024

Extracellular vesicles (EVs) are natural carriers of biomolecules that play a crucial role in cell-to-cell communication and tissue homeostasis under normal pathological conditions, including inflammatory diseases cancer. Since the discovery pro-regenerative immune-modulating properties EVs, EV-based therapeutics have entered clinical trials for conditions such as myocardial infarction autoimmune diseases, among others. Due to their unique advantages—such superior bioavailability, substantial packaging capacity, ability traverse biological barriers—EVs regarded promising platform targeted drug delivery. However, achieving sufficient accumulation therapeutic agents at target site necessitates larger quantity EVs per dose compared using standalone drugs. This challenge can be addressed by administering doses increasing dosage administration, or enhancing selective cells. In this review, we will discuss methods improve isolation purification approaches enhance cargo packaging—including proteins, RNAs, small-molecule drugs—and technologies displaying targeting ligands on surface facilitate improved targeting. Ultimately, guide applied development novel classes overcoming existing technological challenges.

Language: Английский

Citations

2
Biotherapeutic approaches against cardio-metabolic dysfunctions based on extracellular vesicles DOI Creative Commons
Stéphane Marchal, Ramaroson Andriantsitohaina, Maria Carmen Martínez

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(4), P. 167095 - 167095

Published: Feb. 28, 2024

Among the different pathways involved in cell-to-cell communication, extracellular vesicles (EVs) are defined as key players transport of signalling molecules, such lipids, proteins, and RNA, from originating cells to specific target cells. The biogenesis composition EVs complex confer them a unique ability more effectively reach tissues compared other types synthetic carriers. Owing these properties, have been suggested new therapeutic tools for personalized medicine. Since cardiometabolic diseases reached pandemic proportions, therapies needed be developed. In this context, appear promising against disorders associated with obesity diabetes. This review focuses on latest research preclinical applications diseases, draw primarily our experience area.

Language: Английский

Citations

1
Comparative analysis of nucleic acid delivery systems for gene therapy: assessing viral and non-viral approaches with emphasis on extracellular vesicles DOI Creative Commons
Nizar Y. Saad

Deleted Journal, Journal Year: 2024, Volume and Issue: 01(02), P. 67 - 81

Published: March 4, 2024

In the landscape of modern medicine, nucleic acid therapeutics have emerged as groundbreaking agents, holding promise to revolutionize disease treatment at its genetic roots.These encompass a spectrum approaches, including gene, oligonucleotide drug, and mRNA therapies, each designed modulate gene expression or function.The potential lies in their ability address disorders by directly targeting manipulating underlying material.However, translation therapeutic concepts into effective clinical interventions faces challenges, spanning delivery hurdles, immunogenicity concerns, need for sustained efficacy.This Commentary article navigates complexities associated with therapeutics, particular focus on therapy, explores recent emergence extracellular vesicles solution overcome hurdles delivery.

Language: Английский

Citations

1
Extracellular vesicles isolated from curcumin-medium weakened RKO cell proliferation and migration DOI Open Access
Chunyan Xu, Chengxia Liu

Translational Cancer Research, Journal Year: 2024, Volume and Issue: 13(6), P. 2596 - 2604

Published: June 1, 2024

Background: Curcumin (Cur) is a natural phytochemical that expected to become an indispensable drug for the treatment of colorectal cancer. A comprehensive understanding anti-tumor mechanism Cur will provide better reference its clinical application. This study aimed examine effects extracellular vesicles (EVs) isolated from Cur-medium on RKO cancer cell proliferation, apoptosis, and migration. Methods: cells were cultured in various concentrations Cur-medium, EVs Cur-medium. The identified by transmission electron microscopy western blotting. migration analyzed, as was expression proliferating nuclear antigen (PCNA), Bax, vimentin, E-cadherin. factor κB (NF-κB) p65 also detected. Results: Our results showed weakened proliferation but had no effect apoptosis. suppressed NF-κB EVs. Overall, this revealed exerts suppressing weaken Conclusions: In conclusion, packaging into future.

Language: Английский

Citations

1