Exchange of free and capsule conjugated cyanine dyes between cells DOI
Zhanna V. Kozyreva, Polina A. Demina, Оlga I. Gusliakova

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Fluorescent dyes (especially photoconvertible cyanine dyes) are traditionally used as labels to study single-cell or cell-group interactions and migration. Nevertheless, their application has some disadvantages, such cytotoxicity dye transfer between cells during co-cultivation. The latter can lead serious distortions in research results. At the same time, lack of a worthy alternative explains reasons for hushing up this problem. Here, we propose low-cytotoxicity encapsulated forms 3.5 5.5, enabling intracellular uptake facilitating labeling tracking an efficient existing staining. Only 16.9% myoblasts (C2C12) exchanged compared with 99.7% that free form dyes. Simultaneous several dyes, combined possibility photoconversion, provides multi-color coding individual cells. Encapsulation allows reliable reduces

Language: Английский

Exchange of free and capsule conjugated cyanine dyes between cells DOI
Zhanna V. Kozyreva, Polina A. Demina, Оlga I. Gusliakova

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Fluorescent dyes (especially photoconvertible cyanine dyes) are traditionally used as labels to study single-cell or cell-group interactions and migration. Nevertheless, their application has some disadvantages, such cytotoxicity dye transfer between cells during co-cultivation. The latter can lead serious distortions in research results. At the same time, lack of a worthy alternative explains reasons for hushing up this problem. Here, we propose low-cytotoxicity encapsulated forms 3.5 5.5, enabling intracellular uptake facilitating labeling tracking an efficient existing staining. Only 16.9% myoblasts (C2C12) exchanged compared with 99.7% that free form dyes. Simultaneous several dyes, combined possibility photoconversion, provides multi-color coding individual cells. Encapsulation allows reliable reduces

Language: Английский

Citations

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