Ultrasound Stimulation Modulates Microglia M1/M2 Polarization and Affects Hippocampal Proteomic Changes in a Mouse Model of Alzheimer's Disease DOI Creative Commons
Xinliang Lu,

Wenxian Sun,

Li Leng

et al.

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(11)

Published: Nov. 1, 2024

ABSTRACT Background The effectiveness of ultrasound stimulation in treating Alzheimer's disease (AD) has been reported previous studies, but the underlying mechanisms remain unclear. This study investigated effects on proportion and function microglia different phenotypes, as well levels inflammatory factors. Additionally, it revealed alterations proteomic molecules mouse hippocampus following treatment, aiming to uncover potential new molecular mechanisms. Methods Ultrasound was used stimulate for 30 min per day 5 days stimulation‐treated group. Amyloid plaque deposition measured using immunofluorescence staining. M1 M2 type were labeled immunofluorescent double staining, ratio calculated. Aβ42, IL‐10, TNF‐α determined ELISA kits. quantitative proteomics method employed explore changes hippocampal proteins. Results treatment reduced average fluorescence intensity amyloid plaques concentration Aβ42. Compared AD group, resulted a 14% reduction 12% increase microglia. anti‐inflammatory factor IL‐10 significantly increased Proteomics analysis 753 differentially expressed proteins between groups, with most being enriched oxidative phosphorylation pathway mitochondria. activity cytochrome c oxidase, involved phosphorylation, after treatment. Conclusions affects microglial polarization, reduces load, enhances factors APP/PS1 mice. reveals mechanism behind stimulation‐induced modulation polarization may be related mitochondrial phosphorylation.

Language: Английский

Surgical Treatments of Parkinson’s Disease DOI

Ariane Veilleux Carpentier,

Michael S. Okun

Neurologic Clinics, Journal Year: 2025, Volume and Issue: 43(2), P. 383 - 397

Published: Jan. 22, 2025

Language: Английский

Citations

0
The future: Stem cells? Current clinical trials using stem cells for dopaminergic cell replacement DOI
Gesine Paul, Asuka Morizane, Agnete Kirkeby

et al.

International review of movement disorders, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

0
Comment prendre en charge un tremblement pharmaco-résistant handicapant ? DOI
Mickael Aubignat, Mélissa Tir, Michel Lefranc

et al.

Pratique Neurologique - FMC, Journal Year: 2024, Volume and Issue: 15(4), P. 238 - 245

Published: Oct. 29, 2024

Citations

0
Ultrasound Stimulation Modulates Microglia M1/M2 Polarization and Affects Hippocampal Proteomic Changes in a Mouse Model of Alzheimer's Disease DOI Creative Commons
Xinliang Lu,

Wenxian Sun,

Li Leng

et al.

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(11)

Published: Nov. 1, 2024

ABSTRACT Background The effectiveness of ultrasound stimulation in treating Alzheimer's disease (AD) has been reported previous studies, but the underlying mechanisms remain unclear. This study investigated effects on proportion and function microglia different phenotypes, as well levels inflammatory factors. Additionally, it revealed alterations proteomic molecules mouse hippocampus following treatment, aiming to uncover potential new molecular mechanisms. Methods Ultrasound was used stimulate for 30 min per day 5 days stimulation‐treated group. Amyloid plaque deposition measured using immunofluorescence staining. M1 M2 type were labeled immunofluorescent double staining, ratio calculated. Aβ42, IL‐10, TNF‐α determined ELISA kits. quantitative proteomics method employed explore changes hippocampal proteins. Results treatment reduced average fluorescence intensity amyloid plaques concentration Aβ42. Compared AD group, resulted a 14% reduction 12% increase microglia. anti‐inflammatory factor IL‐10 significantly increased Proteomics analysis 753 differentially expressed proteins between groups, with most being enriched oxidative phosphorylation pathway mitochondria. activity cytochrome c oxidase, involved phosphorylation, after treatment. Conclusions affects microglial polarization, reduces load, enhances factors APP/PS1 mice. reveals mechanism behind stimulation‐induced modulation polarization may be related mitochondrial phosphorylation.

Language: Английский

Citations

0