Maternal nitric oxide homeostasis impacts female gametophyte development under optimal and stress conditions

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(6), P. 2201 - 2218

Published: Feb. 20, 2024

Abstract In adverse environments, the number of fertilizable female gametophytes (FGs) in plants is reduced, leading to increased survival remaining offspring. How maternal plant perceives internal growth cues and external stress conditions alter FG development remains largely unknown. We report that homeostasis signaling molecule nitric oxide (NO) plays a key role controlling under both optimal conditions. NO precisely regulated by S-nitrosoglutathione reductase (GSNOR). Prior fertilization, GSNOR protein exclusively accumulated sporophytic tissues indirectly controls Arabidopsis (Arabidopsis thaliana). null mutants, species degenerating nucellus, auxin efflux into developing was restricted, which inhibited development, resulting reduced fertility. Importantly, restoring expression maternal, but not gametophytic tissues, or increasing substrate significantly proportion normal FGs Furthermore, overexpression added fertility drought salt stress. These data indicate critical transport control turn determine seed yield. Understanding this aspect could contribute mediating yield loss

Language: Английский

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Cellular and molecular bases of lateral root initiation and morphogenesis

Current Opinion in Plant Biology, Journal Year: 2021, Volume and Issue: 65, P. 102115 - 102115

Published: Nov. 4, 2021

Language: Английский

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ZmNRT1.1B (ZmNPF6.6) determines nitrogen use efficiency via regulation of nitrate transport and signalling in maize

Plant Biotechnology Journal, Journal Year: 2023, Volume and Issue: 22(2), P. 316 - 329

Published: Oct. 2, 2023

Summary Nitrate (NO 3 − ) is crucial for optimal plant growth and development often limits crop productivity under low availability. In comparison with model Arabidopsis, the molecular mechanisms underlying NO acquisition utilization remain largely unclear in maize. particular, only a few genes have been exploited to improve nitrogen use efficiency (NUE). Here, we demonstrated that ‐inducible ZmNRT1.1B ( ZmNPF6.6 positively regulated ‐dependent NUE We showed tandem duplicated proteoform ZmNRT1.1C irrelevant maize seedling supply; however, loss of function significantly weakened adequate supply both hydroponic field conditions. The 15 N‐labelled absorption assay indicated mediated high‐affinity ‐transport root‐to‐shoot translocation. Transcriptome analysis further showed, upon supply, promotes cytoplasmic‐to‐nuclear shuttling ZmNLP3.1 (ZmNLP8), which co‐regulates expression involved response, cytokinin biosynthesis carbon metabolism. Remarkably, overexpression modern hybrids improved grain yield N‐limiting fields. Taken together, our study revealed role transport signalling offers valuable genetic resource breeding N efficient high‐yield cultivars.

Language: Английский

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Convergently selected NPF2.12 coordinates root growth and nitrogen use efficiency in wheat and barley

New Phytologist, Journal Year: 2023, Volume and Issue: 238(5), P. 2175 - 2193

Published: Feb. 18, 2023

Understanding the genetic and molecular function of nitrate sensing acquisition across crop species will accelerate breeding cultivars with improved nitrogen use efficiency (NUE). Here, we performed a genome-wide scan using wheat barley accessions characterized under low high N inputs that uncovered NPF2.12 gene, encoding homolog Arabidopsis transceptor NRT1.6 other low-affinity transporters belong to MAJOR FACILITATOR SUPERFAMILY. Next, it is shown variations in promoter correlated altered transcript levels where decreased gene expression was measured availability. Multiple field trials revealed significantly enhanced content leaves grains NUE presence elite allele TaNPF2.12TT grown conditions. Furthermore, reductase NIA1 up-regulated npf2.12 mutant upon concentrations, thereby resulting elevated nitric oxide (NO) production. This increase NO higher root growth, uptake, translocation observed when compared wild-type. The presented data indicate haplotype alleles are convergently selected by inactivation indirectly contribute growth activating signaling

Language: Английский

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HD-Zip II transcription factors control distal stem cell fate in Arabidopsis roots by linking auxin signaling to the FEZ/SOMBRERO pathway

Development, Journal Year: 2024, Volume and Issue: 151(8)

Published: April 2, 2024

ABSTRACT In multicellular organisms, specialized tissues are generated by specific populations of stem cells through cycles asymmetric cell divisions, where one daughter undergoes differentiation and the other maintains proliferative properties. Arabidopsis thaliana roots, columella – a gravity-sensing tissue that protects defines position niche represents typical example whose organization is exclusively determined balance between proliferation differentiation. The derives from single layer binary fate switch precisely controlled multiple, independent regulatory inputs. Here, we show HD-Zip II transcription factors (TFs) HAT3, ATHB4 AHTB2 redundantly regulate patterning in root. TFs promote acting as effectors FEZ/SMB circuit and, at same time, interfering with auxin signaling to counteract hormone-induced Overall, our work shows connect two opposing parallel inputs fine-tune cells.

Language: Английский

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Advancing Sustainability in Pelargonium Nursery Management: Scientific Exploration of an Innovative Prolonged Cold Storage System for Cuttings

Agronomy, Journal Year: 2025, Volume and Issue: 15(4), P. 907 - 907

Published: April 6, 2025

Cold storage of pelargonium cuttings addresses the issue nonoverlapping production seasons in Central Europe, where are harvested from stock plants December, but rooting begins mid-February/March. Here, we show an innovative system for using nature-based solutions. We compared post-delivery unrooted paperpots maintained greenhouses (8/6 °C day/night) to standard immediately after delivery. Key factors included genotype (Pelargonium zonale, P. peltatum, and hybrids), four delivery weeks (48–51), two growing (2021 2022), duration (up 4 weeks). Genotype strongly influenced cold tolerance, with peltatum enduring up without significant loss efficiency, unlike zonale. The success depended on plants’ quality nutritional status: higher nitrogen content 2022 2021 was associated reduced zonale hybrids. Neither timing nor residual ethephon affected outcomes storage. This study demonstrates that storing this method preserves while optimizing efficiency by reducing nursery space, water, fertilizer, pesticide use. shift practices reduces per-unit costs enhances economic viability bedding young plant producers.

Language: Английский

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Effects of Water–Nitrogen Coupling on Root Distribution and Yield of Summer Maize at Different Growth Stages

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1278 - 1278

Published: April 22, 2025

This research investigates the influence of water–nitrogen coupling on soil water content, nitrogen dynamics, and root distribution in farmland, along with interactions among water, transport, distribution, crop yield. A field experiment was conducted under moderate drought stress (50–60% capacity) three application rates (100, 200, 300 kg·ha−1, split-applied at 50% during sowing jointing stage, labeled as N1, N2, N3) two critical growth stages (jointing stage P1 tasseling-silking P2) maize (Denghai 605). The results demonstrated that morphological parameters exhibited trend N2 > N1 N3 different treatments. Compared to low (N1) decreased by 35.01–49.60% average, whereas high (N3) led a reduction 49.93–61.37%. treatment consistently maintained greater uptake, highest yield 13,336 kg·ha−1 observed CKN2 treatment, representing increases 16.1% 9.2% compared P1N2 P2N2 treatments, respectively. Drought (P1) inhibited development more severely than (P2), demonstrating bidirectional adaptation strategy characterized deeper vertical penetration increased horizontal expansion imbalance. Correlation analysis revealed positive correlation between nutrient content indicators. At same time, characteristic values were significantly negatively correlated (p < 0.05). Appropriate management effectively stimulated growth, mitigated leaching risks, improved These findings offer theoretical foundation for optimizing production within Yellow River Basin.

Language: Английский

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Mutation of OsPIN1b by CRISPR/Cas9 Reveals a Role for Auxin Transport in Modulating Rice Architecture and Root Gravitropism

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(16), P. 8965 - 8965

Published: Aug. 11, 2022

The distribution and content of auxin within plant tissues affect a variety important growth developmental processes. Polar transport (PAT), mainly mediated by influx efflux transporters, plays vital role in determining maxima gradients plants. carrier PIN-FORMED (PIN) family is one the major protein families involved PAT. Rice (Oryza sativa L.) genome possesses 12 OsPIN genes. However, detailed functions genes regulating rice architecture gravity response are less well understood. In present study, OsPIN1b was disrupted CRISPR/Cas9 technology, its roles modulating root gravitropism were investigated. Tissue-specific analysis showed that expressed roots, stems sheaths at seedling stage, transcript abundance progressively decreased during stages. Expression could be quickly greatly induced NAA, indicating played IAA homeostasis disturbed ospin1b mutants, as evidenced changed sensitivity shoot to NAA NPA treatment, respectively. Mutation resulted pleiotropic phenotypes, including shoots primary reduced adventitious number seedlings, shorter narrower leaves, increased leaf angle, more tiller height panicle length late stage. Moreover, mutants displayed curly phenotype cultured with tap water regardless lighting conditions, while nutrient solution culture partially rescue light almost completely abolish this darkness, involvement integration signals regulation. Additionally, amyloplast sedimentation impaired peripheral tiers cap columella cell, it not main contributor abnormal gravitropism. These data suggest only but also signals.

Language: Английский

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Low nitrogen supply inhibits root growth but prolongs lateral root lifespan in cotton

Industrial Crops and Products, Journal Year: 2022, Volume and Issue: 189, P. 115733 - 115733

Published: Oct. 13, 2022

Language: Английский

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Toxicological Effects of Mercury-Induced Biochemical Alterations in Curry Leaves (Murraya koenigii) Plants

AgroEnvironmental Sustainability, Journal Year: 2025, Volume and Issue: 3(1), P. 10 - 21

Published: March 15, 2025

Heavy metals, including mercury (Hg), accumulate in the environment via atmospheric deposition, aquatic transport, and terrestrial pathways, eventually settling soil sediments. Once these metals become bioavailable, they pose significant ecological toxicological risks. Upon exposure, plants absorb them, leading to harmful agronomic, physiological, biochemical effects. The present study aims assess effects of on alterations Murraya koenigii (curry leaves) plants. M. were assigned four groups: Group 1 (control) uncontaminated soil, Groups 2, 3, 4 exposed 50 mg, 100 200 mg Hg, respectively. All kept under controlled environmental conditions promote optimal growth. results revealed that elevated concentrations significantly impaired critical growth parameters, seed germination, root shoot length, fresh dry weight, vigour index, all which reflect suppressed plant productivity. Biochemical analysis further demonstrated substantial reductions primary metabolites, such as carbohydrates proteins, with most pronounced decreases observed at higher concentrations. These suggest Hg-induced oxidative stress causes cellular damage, disruption nutrient assimilation, disturbances enzyme activity. Additionally, chlorophyll a, b, total content observed, indicating photosynthetic capacity. Overall, findings underscore detrimental metabolic processes, highlighting its potential cause long-term inhibition dysfunction, broader implications for productivity, cycling, ecosystem health.

Language: Английский

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