Transposon-triggered epigenetic chromatin dynamics modulate EFR-related pathogen response DOI Creative Commons
Regina Mencia, A. Arce,

Candela Houriet

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 9, 2023

Summary Infectious diseases drive the evolution of wild plants and impact yield in crop plants. Like animals, can sense biotic threats via conserved pathogen-associated patterns (PAMPs). Since an overly robust immune response harm plants, understanding mechanisms for tuning defense responses to appropriate level is vital as we endeavor develop pathogen-resistant crops. In this paper, studied Arabidopsis pattern recognition receptor (PRR) EFR, which senses bacterial EF-Tu. An inverted-repeat transposon ( Ea-IR ) between EFR neighboring XI-k locus controls local chromatin organization, promoting formation a repressive loop. Upon pathogen infection, landscape around Xl-k dynamically changes allow increased transcription. Chromatin opening facilitates passage RNA polymerase II across gene termination site, leading longer transcript that includes sequences. Dicer-like (DCL) enzymes process into small RNAs (sRNAs), reset topology state, attenuating, turn, response, reminiscent attenuation signaling other systems. From evolutionary point view, found natural accessions missing have constitutive " -open" configuration correlates with higher basal levels background resistance pathogens. Collectively, our study offers evidence scenario transposon, expression interact fine-tune responses, both during course infection evolution. Similar gene-associated IRs crops could provide valuable non-coding targets genome editing or assisted plant breeding programs.

Language: Английский

Transposon-triggered epigenetic chromatin dynamics modulate EFR-related pathogen response DOI Creative Commons
Regina Mencia, A. Arce,

Candela Houriet

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 9, 2023

Summary Infectious diseases drive the evolution of wild plants and impact yield in crop plants. Like animals, can sense biotic threats via conserved pathogen-associated patterns (PAMPs). Since an overly robust immune response harm plants, understanding mechanisms for tuning defense responses to appropriate level is vital as we endeavor develop pathogen-resistant crops. In this paper, studied Arabidopsis pattern recognition receptor (PRR) EFR, which senses bacterial EF-Tu. An inverted-repeat transposon ( Ea-IR ) between EFR neighboring XI-k locus controls local chromatin organization, promoting formation a repressive loop. Upon pathogen infection, landscape around Xl-k dynamically changes allow increased transcription. Chromatin opening facilitates passage RNA polymerase II across gene termination site, leading longer transcript that includes sequences. Dicer-like (DCL) enzymes process into small RNAs (sRNAs), reset topology state, attenuating, turn, response, reminiscent attenuation signaling other systems. From evolutionary point view, found natural accessions missing have constitutive " -open" configuration correlates with higher basal levels background resistance pathogens. Collectively, our study offers evidence scenario transposon, expression interact fine-tune responses, both during course infection evolution. Similar gene-associated IRs crops could provide valuable non-coding targets genome editing or assisted plant breeding programs.

Language: Английский

Citations

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