One stone, two birds: The barley NLR protein MLA3 recognizes the rice blast fungus effector Pwl2 in addition to its cognate effector AVRa3 from barley powdery mildew DOI Creative Commons
Leiyun Yang

The Plant Cell, Journal Year: 2023, Volume and Issue: 36(2), P. 223 - 224

Published: Oct. 31, 2023

Language: Английский

Wheat powdery mildew resistance: from gene identification to immunity deployment DOI Creative Commons

Shenghao Zou,

Yang Xu, Qianqian Li

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 18, 2023

Powdery mildew is one of the most devastating diseases on wheat and caused by obligate biotrophic phytopathogen Blumeria graminis f. sp. tritici ( Bgt ). Due to complexity large genome its close relatives, identification powdery resistance genes had been hampered for a long time until recent progress in large-scale sequencing, genomics, rapid gene isolation techniques. Here, we describe summarize current advances resistance, emphasizing discoveries about conferring similarity, diversity molecular function those genes. Multilayered could be used counteracting , including durable, broad spectrum but partial as well race-specific mostly complete mediated nucleotide-binding leucine rich repeat domain (NLR) proteins. In addition above mentioned layers, manipulation susceptibility (S) negative regulator may represent another layer that can durable broad-spectrum wheat. We propose it promising develop effective strategies combat simultaneous deployment multilayered immunity.

Language: Английский

Citations

7
NbPTR1 confers resistance against Pseudomonas syringae pv. actinidiae in kiwifruit DOI Creative Commons
Shin‐Mei Yeh, Minsoo Yoon,

Sidney Scott

et al.

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: 47(11), P. 4101 - 4115

Published: June 20, 2024

Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) causes a devastating canker disease in yellow-fleshed kiwifruit (Actinidia chinensis). The effector HopZ5, which is present all isolates of Psa3 causing global outbreaks pandemic disease, triggers immunity Nicotiana benthamiana and not recognised susceptible A. chinensis cultivars. In search for N. nonhost resistance genes against we found that the nucleotide-binding leucine-rich repeat receptor NbPTR1 HopZ5. RPM1-interacting protein 4 orthologues from formed complex with HopZ5 activity was able to disrupt this interaction. No functional were chinensis. transformed into Psa3-susceptible var. 'Hort16A' plants introduced HopZ5-specific Psa3. Altogether, study suggested expressing viable approach acquiring it provides valuable information engineering otherwise genotypes.

Language: Английский

Citations

1
Structure determinants of DANGEROUS MIX 3, an alpha/beta hydrolase, for triggering NLR-mediated genetic incompatibility in plants DOI Creative Commons
Gijeong Kim, Wei‐Lin Wan, Nayun Kim

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

SUMMARY Genetic incompatibility occurs when a mismatched pair of plant immune components mounts autoimmune responses in hybrids. Highly diversified NLR receptors are main culprits the genetic conflict, recognizing host proteins from different origin as trigger. Here, we report molecular mechanism underlying DANGEROUS MIX (DM) autoimmunity, comprising DM2h/RPP1 and its incompatible partner DM3, an alpha/beta hydrolase. Cryo-electron microscopy reveals oligomeric nature two natural DM3 variants trimer dimer configuration. The polymorphism triggering autoimmunity is located at interface, resulting drastic structural differences such that dimerizing helix loop reinforcing interface lost disordered. Structure-function analysis shows integrity but neither maintenance hexamer nor enzymatic activity, key factor contributing to autoimmunity. Our finding pinpoints checkpoints embedded configuration enzyme controls switching activity.

Language: Английский

Citations

0
NbPTR1confers resistance againstPseudomonas syringae pv. actinidiaein kiwifruit DOI Open Access

Shin-Mei Yeh,

Minsoo Yoon,

Sidney Scott

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 10, 2023

Abstract Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) causes a devastating canker disease in yellow-fleshed kiwifruit ( Actinidia chinensis ). The effector HopZ5, which is present all isolates of Psa3 causing global outbreaks pandemic disease, triggers immunity Nicotiana benthamiana and not recognised susceptible A. cultivars. In search for N. non-host resistance genes against we found that the nucleotide-binding leucine-rich repeat receptor NbPTR1 HopZ5. RPM1-interacting protein 4 (RIN4) orthologues from multiple plants, including kiwifruit, were associated with NbPTR1-mediated autoimmunity suppression recognition No functional Nb PTR1 . transformed into Psa3-susceptible var. ‘Hort16A’ plants introduced HopZ5-specific Psa3. Altogether, this study suggested expressing viable approach to acquiring it provides valuable information engineering otherwise genotypes.

Language: Английский

Citations

0
One stone, two birds: The barley NLR protein MLA3 recognizes the rice blast fungus effector Pwl2 in addition to its cognate effector AVRa3 from barley powdery mildew DOI Creative Commons
Leiyun Yang

The Plant Cell, Journal Year: 2023, Volume and Issue: 36(2), P. 223 - 224

Published: Oct. 31, 2023

Language: Английский

Citations

0