New Insight on the Sublethal Effect of Bt-Cry1Ab in Spodoptera litura (Fabricius): Tissular Distribution of Cry1Ab, Ultrastructural Alterations and the Lysosomal Response DOI Creative Commons

Yan‐Jue Wang,

Y. Y. Shen,

Yingdan Xiao

et al.

Insects, Journal Year: 2024, Volume and Issue: 16(1), P. 10 - 10

Published: Dec. 27, 2024

Bt has been applied as a gene source for insect-resistant transgenic crops, which represents efficient control of insect pests. In this study, we evaluated the pesticidal specificity one maize strain, DBN9936, that expresses Cry1Ab protein in Spodoptera litura larvae. The results showed is active against younger larvae while causing sublethal effect on older To further assess biological responses S. under exposure, relative concentrations different tissues fifth instar were investigated, indicating transported along gut and out body via excretion or into hemocoel lead to sequestration by molting. Furthermore, result ultrastructural observation Cry1Ab-treated midgut, namely an increase lysosome number, with lysosomal activity activation simultaneously provides strong indication plays role response exposure.

Language: Английский

RNAi-mediated knockdown of HcCAT2 depresses the adaptive capacity of Hyphantria cunea larvae to cytisine and coumarin DOI
Xue Yan,

Aoying Zhang,

Mingtao Tan

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 295, P. 139593 - 139593

Published: Jan. 7, 2025

Language: Английский

Citations

0
Automated video-tracking analysis of Agriotes obscurus wireworm behaviour before, during and after contact with thiamethoxam- and imidacloprid-treated wheat seeds DOI Creative Commons
Enno Merivee, Anne Must, Karin Nurme

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 28, 2025

Limited studies have highlighted the importance of incorporating behavioural assessments into insecticide efficacy evaluations for wireworm pest control. "For this study, video tracking technology combined with a soil bioassay arena was employed to analyse behaviour Agriotes obscurus wireworms before, during, and after exposure wheat seeds treated neonicotinoid insecticides thiamethoxam imidacloprid at field-relevant concentrations. The analysis identified set key metrics assessing effects these on wireworms. results showed that exhibited neutral attractancy towards A brief period feeding followed by rapid intoxication minimised damage seeds. Furthermore, demonstrated specific form resistance neonicotinoids did not rely sensory input. In insects, speed intoxication, accompanied drastic changes in behaviour, ensured they received sublethal rather than lethal dose insecticide. fully recovered from all abnormalities within week, none died 20 days following exposure. conclusion, method provides efficient means intended management, offering valuable insights prior more resource-intensive costly field trials.

Language: Английский

Citations

0
Azoxystrobin-Induced Physiological and Biochemical Alterations in Apis mellifera Workers of Different Ages DOI Creative Commons

Xinle Duan,

Wenlong Tong,

Bowen Tao

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 449 - 449

Published: April 24, 2025

Fungal diseases of agricultural crops cause severe economic losses to the growers. For control these diseases, azoxystrobin is one recommended fungicides. This fungicide systemic in action and expected reach floral part treated crop its residue pollen nectar, natural food sources honey bees, which could be collected fed on by thus affecting their health. The purpose this study was determine physiological chemical changes caused bee workers (Apis mellifera L). Workers at 1, 8, 21 days old were with 125, 167, 250 mg/L concentrations for seven days; survival rates, activities carboxylesterase (CarE), glutathione S-transferases (GSTs), cytochrome P450 enzyme (CYP450), catalase (CAT), superoxide dismutase (SOD) enzymes, expression levels immune (Aba, Api, Def1, Hym) nutrition genes (Ilp1, Ilp2, Vg) detected. Our findings revealed that affected workers, particularly 1- 21-day-old who responded stress increased detoxification protective might have costs. Additionally, genes, a decreased trend compared 8-day-old leading reduced resistance external stressors mortality rates. These provide important insights into adverse effects different ages emphasize potential risks colony stability individual recommends an urgent ban such harmful being used fungi agriculture, especially during plant flowering.

Language: Английский

Citations

0
Transcriptome analysis reveals glycometabolism and antioxidation-related genes involved in the antifungal immune response of Spodoptera frugiperda larvae against Beauveria bassiana infection DOI Creative Commons
Yongkai Mao, Jiequn Yi, Yinjie Cheng

et al.

European Journal of Entomology, Journal Year: 2024, Volume and Issue: 121, P. 310 - 323

Published: Sept. 11, 2024

Language: Английский

Citations

0
Insights into the Detoxification of Spruce Monoterpenes by the Eurasian Spruce Bark Beetle DOI Open Access
Aisha Naseer, Vivek Vikram Singh,

Gothandapani Sellamuthu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10209 - 10209

Published: Sept. 23, 2024

Plant defence mechanisms, including physical barriers like toughened bark and chemical defences allelochemicals, are essential for protecting them against pests. Trees allocate non-structural carbohydrates (NSCs) to produce secondary metabolites monoterpenes, which increase during biotic stress fend off pests the Eurasian spruce beetle, ESBB (

Language: Английский

Citations

0
New Insight on the Sublethal Effect of Bt-Cry1Ab in Spodoptera litura (Fabricius): Tissular Distribution of Cry1Ab, Ultrastructural Alterations and the Lysosomal Response DOI Creative Commons

Yan‐Jue Wang,

Y. Y. Shen,

Yingdan Xiao

et al.

Insects, Journal Year: 2024, Volume and Issue: 16(1), P. 10 - 10

Published: Dec. 27, 2024

Bt has been applied as a gene source for insect-resistant transgenic crops, which represents efficient control of insect pests. In this study, we evaluated the pesticidal specificity one maize strain, DBN9936, that expresses Cry1Ab protein in Spodoptera litura larvae. The results showed is active against younger larvae while causing sublethal effect on older To further assess biological responses S. under exposure, relative concentrations different tissues fifth instar were investigated, indicating transported along gut and out body via excretion or into hemocoel lead to sequestration by molting. Furthermore, result ultrastructural observation Cry1Ab-treated midgut, namely an increase lysosome number, with lysosomal activity activation simultaneously provides strong indication plays role response exposure.

Language: Английский

Citations

0