miR858a-encoded peptide, miPEP858a, interacts with the miR858a promoter and requires the C-terminus for associated functions DOI
Himanshi Gautam, Ashish Sharma,

Anwesha Anyatama

et al.

PLANT PHYSIOLOGY, Journal Year: 2025, Volume and Issue: 198(1)

Published: April 30, 2025

MicroRNAs (miRNAs) are key regulators of gene expression and typically processed from primary transcripts (pri-miRNAs). Recent discoveries highlight that certain pri-miRNAs also encode miRNA-encoded peptides (miPEPs), which influence miRNA function. However, the molecular mechanisms underlying miPEP activity, including specific domains or essential amino acid residues required for their function, remain largely unexplored. In this study, we elucidated pri-miR858a-derived peptide, miPEP858a, directly interacts with promoter MIR858 in Arabidopsis (Arabidopsis thaliana). Notably, C-terminal region composed 14 residues, is critical its functionality. Through DNA-protein interaction assays, yeast 1-hybrid, chromatin immunoprecipitation (ChIP-qPCR), electrophoretic mobility shift assay, promoter-reporter analyses, demonstrated miPEP858a binds to a within promoter. Exogenous application synthetic peptide corresponding resulted enhanced expression, leading phenotypic changes similar those observed full-length miPEP858a. Moreover, truncated was able complement mutant plants lacking endogenous emphasizing role regulating miR858a downstream target genes involved flavonoid biosynthesis plant development. These findings suggest may not be necessary biological being sufficient modulate expression. This discovery reveals opportunities identifying functional other miPEPs, potentially reducing synthesis costs, offering more efficient strategy enhancing agronomic traits crop without need complex biotechnological interventions.

Language: Английский

Promising Biotechnological Applications of the Artificial Derivatives Designed and Constructed from Plant microRNA Genes DOI Creative Commons
Tatiana N. Erokhina, Ekaterina V. Ryabukhina,

Irina Lyapina

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(3), P. 325 - 325

Published: Jan. 22, 2025

MicroRNAs (miRNAs) are small regulatory RNAs that expressed in a tissue-specific manner during the development of plants and animals. The genes miRNAs have been found to produce following two products: (i) primary transcripts these (pri-miRNA) processed give rise mature miRNA, (ii) some cases, pri-miRNA molecules can be translated form peptides, named as miPEPs. Gene silencing by artificial microRNAs (amiRNAs) is one potential crucial methods for regulation desired improve horticultural plants. Likewise, external application chemically synthesized miPEPs may help resist biotic/abiotic stresses grow faster. These potent reliable derivatives miRNA applied improving useful traits crop This review summarizes progress research on gene involved regulating plant development, virus pest diseases, abiotic stress resistance pathways. We also briefly discuss molecular mechanisms relevant target future breeding In general, this researchers who implementing amiRNA miPEP accelerating programs developmental studies

Language: Английский

Citations

0
MIR408-encoded peptide, miPEP408, regulates cadmium stress response through sulfur assimilation pathway DOI
Ravi Shankar Kumar,

Tapasya Datta,

Hiteshwari Sinha

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 138420 - 138420

Published: April 1, 2025

Language: Английский

Citations

0
miR858a-encoded peptide, miPEP858a, interacts with the miR858a promoter and requires the C-terminus for associated functions DOI
Himanshi Gautam, Ashish Sharma,

Anwesha Anyatama

et al.

PLANT PHYSIOLOGY, Journal Year: 2025, Volume and Issue: 198(1)

Published: April 30, 2025

MicroRNAs (miRNAs) are key regulators of gene expression and typically processed from primary transcripts (pri-miRNAs). Recent discoveries highlight that certain pri-miRNAs also encode miRNA-encoded peptides (miPEPs), which influence miRNA function. However, the molecular mechanisms underlying miPEP activity, including specific domains or essential amino acid residues required for their function, remain largely unexplored. In this study, we elucidated pri-miR858a-derived peptide, miPEP858a, directly interacts with promoter MIR858 in Arabidopsis (Arabidopsis thaliana). Notably, C-terminal region composed 14 residues, is critical its functionality. Through DNA-protein interaction assays, yeast 1-hybrid, chromatin immunoprecipitation (ChIP-qPCR), electrophoretic mobility shift assay, promoter-reporter analyses, demonstrated miPEP858a binds to a within promoter. Exogenous application synthetic peptide corresponding resulted enhanced expression, leading phenotypic changes similar those observed full-length miPEP858a. Moreover, truncated was able complement mutant plants lacking endogenous emphasizing role regulating miR858a downstream target genes involved flavonoid biosynthesis plant development. These findings suggest may not be necessary biological being sufficient modulate expression. This discovery reveals opportunities identifying functional other miPEPs, potentially reducing synthesis costs, offering more efficient strategy enhancing agronomic traits crop without need complex biotechnological interventions.

Language: Английский

Citations

0