Biology Methods and Protocols,
Journal Year:
2024,
Volume and Issue:
9(1)
Published: Jan. 1, 2024
Determining
the
number
of
viable
cells
by
calculating
colony-forming
units
is
time-consuming.
The
evaluation
mixed
biofilms
consisting
different
species
particularly
problematic.
Therefore,
aim
this
study
was
to
optimize
a
molecular
method-propidium
monoazide
quantitative
polymerase
chain
reaction
(PMA-qPCR)-for
accurate
and
consistent
differentiation
between
living
dead
cells.
In
practical
experimental
example,
genome
copies
representing
determined
in
biofilm
From Chemistry Towards Technology Step-By-Step,
Journal Year:
2023,
Volume and Issue:
4(3), P. 76 - 80
Published: Sept. 23, 2023
The
article
considers
the
synthesis
of
meso-tetrakis(1'-methyl-pyrid-4-yl)porphyrin
tetratosylate
and
meso-tetrakis(1'-methyl-carboxymethylpyrid-4-yl)porphyrin
tetrabromide.
authors
qualified
porphyrin
ligands
in
terms
electron
1H
NMR
spectroscopy.
research
determines
decrease
freezing
point
(ΔTfr)
aqueous
solutions
porphyrins,
as
well
a
model
N-methyl-pyridinium
salt
(1
methyl-pyridinium
iodide).
We
used
obtained
experimental
values
ΔT3
to
determine
isotonic
coefficient.
results
indicate
compounds
are
almost
completely
dissociated
indicated
concentration
range
dilute
solutions.
