Enzymatic modification of raw corn starch using thermostable Glucoamylase from Thermothelomyces thermophilus

Grain & Oil Science and Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Engineered Folding and Secretion for Expression Improvement of α‐Amylase in Komagataella Phaffii

Food Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

ABSTRACT α‐Amylase is the second most widely produced enzyme globally, with diverse applications in fields of food, pharmaceutical, bioenergy, papermaking, etc. However, natural α‐amylase often fails to withstand extreme conditions encountered industrial processes, such as low pH and high temperatures. Previous studies identified an derived from deep‐sea sources resistance pH, subsequent amino acid mutations well enhanced its thermal stability. Nevertheless, advantageous mutant exhibited expression levels Escherichia coli , highlighting need for a more suitable host. In this study, engineered host, Komagataella phaffii was involved heterologous production α‐amylase. High‐efficiency signal peptides were screened multi‐copy integrant strains constructed achieve high‐yield strain. A total 31 key chaperones 11 vesicle transport factors further investigated facilitate protein folding secretion, which resulted 3.4‐fold increase production. Finally, batch fermentation 3‐L bioreactor achieved maximum activity 2.5 × 10 4 U/mL. This study demonstrates development strain potential applications, offering valuable insights strategies engineering high‐producing other enzymes.

Language: Английский

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Recent Advances in Microbial Enzyme Applications for Sustainable Textile Processing and Waste Management

Sci, Journal Year: 2025, Volume and Issue: 7(2), P. 46 - 46

Published: April 9, 2025

Microbial enzymes have revolutionised the textile industry by replacing harmful chemicals with eco-friendly alternatives, enhancing processes such as desizing, scouring, dyeing, finishing, and promoting water conservation while reducing pollution. This review explores role of like amylases, pectinases, cellulases, catalases, laccases, peroxidases in sustainable processing, focusing on their ability to mitigate environmental pollution from effluents. The also examines types characteristics hazardous waste evaluates traditional treatment methods, highlighting alternatives microbial enzyme treatments for effluent treatment. Recent advancements recombinant technology, including engineering immobilisation techniques enhance stability, reusability, catalytic performance, are explored. Additionally, potential extremozymes processing is explored, emphasising stability under harsh industrial conditions. Strategies through enzyme-based presented, principles circular economy. addresses challenges scalability, cost, process optimisation, proposing solutions outlining future directions widespread adoption production management. underscores transformative achieving greener manufacturing practices.

Language: Английский

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Effects of fermented corn cob on growth performance, digestive enzyme, immune response, and gene expression of Nile tilapia (Oreochromis niloticus) raised in biofloc system

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110413 - 110413

Published: May 1, 2025

Language: Английский

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