Identification and Characterization of Neuropeptides and Their G Protein-Coupled Receptors (GPCRs) in the Cowpea Aphid Aphis craccivora DOI Creative Commons
Xiao Li, Long Du,

Xiaojing Jiang

et al.

Frontiers in Endocrinology, Journal Year: 2020, Volume and Issue: 11

Published: Sept. 17, 2020

Neuropeptides are the most abundant and diverse signal molecules in insects. They act as neurohormones neuromodulators to regulate physiology behaviour of The majority neuropeptides initiate downstream signalling pathways through binding G protein-coupled receptors (GPCRs) on cell surface. In this study, RNA-seq technology bioinformatics were used search for genes encoding their GPCRs cowpea aphid Aphis craccivora. And expression these at different developmental stages A. craccivora was analysed by quantitative real-time PCR (qRT-PCR). A total 40 candidate neuropeptide precursors identified from transcriptome data, which is roughly equivalent number that have been reported other On basis, software analysis combined with homologous prediction estimated there could be more than 60 mature biological activity. addition, 46 obtained, belong rhodopsin-like (A-family GPCRs), including 21 families 7 orphan receptors, 6 secretin-like (B-family diuretic hormone 31 (DH31), DH44 pigment-dispersing factor (PDF) receptors. Compared holometabolous insects such Drosophila melanogaster, coding sulfakinin, corazonin, arginine vasopressin-like peptide (AVLP) trissin corresponding not found It speculated likely lacks above pathways, consistent Acyrthosiphon pisum loss may a common feature aphids. profiling revealed GPCR differentially expressed wing morphs. This study will help deepen our understanding systems aphids, thus laying foundation development new methods control targeting systems.

Language: Английский

Evolutionarily conserved roles of cholecystokinin signaling DOI
Dick R. Nässel, Shun‐Fan Wu

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 21 - 70

Published: Jan. 1, 2025

Language: Английский

Citations

0
Knockdown of Oatp and Mdr Transporter Genes Enhances Cardenolide Toxicity to the Noncardenolide Feeder, Nilaparvata lugens DOI

Zengxin Li,

Meng Zhang, Li‐Ya Qiao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Insect adaptation to toxic cardenolides has been a model system study convergent evolution between plants and herbivores. It was hypothesized that Organic anion-transporting polypeptides (Oatps) multidrug transporters (Mdrs) provide basal resistance against in nonadapted insect. However, there is still little evidence support this hypothesis. A noncardenolide feeder an important rice pest, Nilaparvata lugens, were used test Bioassays revealed two representative cardenolides, ouabain, digoxin, demonstrated significant toxicity N. lugens. The suppression of the expression levels six Oatp genes three Mdr by RNAi did not result any lethal phenotype knockdown certain significantly enhances oral ouabain digoxin findings our Oatps Mdrs are key lugens protection dietary cardenolides.

Language: Английский

Citations

0
CCK(-like) and receptors: Structure and phylogeny in a comparative perspective DOI
Na Yu, Guy Smagghe

General and Comparative Endocrinology, Journal Year: 2014, Volume and Issue: 209, P. 74 - 81

Published: May 16, 2014

Language: Английский

Citations

38
Characterization and Expression Profiling of Neuropeptides and G-Protein-Coupled Receptors (GPCRs) for Neuropeptides in the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae) DOI Open Access
Zhengbing Wang, Wenwu Zhou,

Muhammad Salman Hameed

et al.

International Journal of Molecular Sciences, Journal Year: 2018, Volume and Issue: 19(12), P. 3912 - 3912

Published: Dec. 6, 2018

Neuropeptides are endogenous active substances that widely exist in multicellular biological nerve tissue and participate the function of nervous system, most them act on neuropeptide receptors. In insects, neuropeptides their receptors play important roles controlling a multitude physiological processes. this project, we sequenced transcriptome from twelve tissues Asian citrus psyllid, Diaphorina citri Kuwayama. A total 40 candidate genes 42 receptor were identified. Among genes, 35 belong to A-family (or rhodopsin-like), four B-family secretin-like), three leucine-rich repeat-containing G-protein-coupled The expression profile 82 across developmental stages was determined by qRT-PCR. Our study provides first investigation D. citri, which may key regulating physiology behaviors citri.

Language: Английский

Citations

37
Identification and Characterization of Neuropeptides and Their G Protein-Coupled Receptors (GPCRs) in the Cowpea Aphid Aphis craccivora DOI Creative Commons
Xiao Li, Long Du,

Xiaojing Jiang

et al.

Frontiers in Endocrinology, Journal Year: 2020, Volume and Issue: 11

Published: Sept. 17, 2020

Neuropeptides are the most abundant and diverse signal molecules in insects. They act as neurohormones neuromodulators to regulate physiology behaviour of The majority neuropeptides initiate downstream signalling pathways through binding G protein-coupled receptors (GPCRs) on cell surface. In this study, RNA-seq technology bioinformatics were used search for genes encoding their GPCRs cowpea aphid Aphis craccivora. And expression these at different developmental stages A. craccivora was analysed by quantitative real-time PCR (qRT-PCR). A total 40 candidate neuropeptide precursors identified from transcriptome data, which is roughly equivalent number that have been reported other On basis, software analysis combined with homologous prediction estimated there could be more than 60 mature biological activity. addition, 46 obtained, belong rhodopsin-like (A-family GPCRs), including 21 families 7 orphan receptors, 6 secretin-like (B-family diuretic hormone 31 (DH31), DH44 pigment-dispersing factor (PDF) receptors. Compared holometabolous insects such Drosophila melanogaster, coding sulfakinin, corazonin, arginine vasopressin-like peptide (AVLP) trissin corresponding not found It speculated likely lacks above pathways, consistent Acyrthosiphon pisum loss may a common feature aphids. profiling revealed GPCR differentially expressed wing morphs. This study will help deepen our understanding systems aphids, thus laying foundation development new methods control targeting systems.

Language: Английский

Citations

28