Nanoparticle-Mediated dsRNA Delivery Used as a Broad-Spectrum Synergistic Nanonucleic Acid Adjuvant to Control Sogatella furcifera

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

The enhanced detoxification capacity caused by the induction or constitutive overexpression of metabolic enzyme genes has complicated sustainable and efficient pest control strategies. This study clarified effects two P450 genes, CYP4CE3 CYP6FJ3, on multiple insecticides in Sogatella furcifera. chimeric double-stranded RNA (ch-dsRNA) these was obtained using L4440-HT1115 (DE3) RNase III- system, interference (RNAi) effectiveness ch-dsRNA verified microinjection. MON-NH2 subsequently used to deliver dsRNA as a synergist (MON-NH2@ch-dsRNA) effectively protect from nuclease degradation stably inhibit CYP6FJ3 expression through insect sap-feeding. Compared with single insecticide application, addition MON-NH2@ch-dsRNA led an increase mortality rates imidacloprid, clothianidin, dinotefuran, sulfoxaflor 22.22-38.90%, synergistic enhancement nitenpyram, yielding ratio 1.90-fold. Moreover, field experiment results revealed that effect nitenpyram significantly improved 14.53 10.30% days 3 7, respectively, following application MON-NH2@ch-dsRNA. Therefore, can be nucleic acid nanosynergist broad-spectrum controlling S.

Language: Английский

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Multiple detoxification enzyme mediates resistance to anticholinesterase insecticides in Cnaphalocrocis medinalis (Lepidoptera: Crambidae)

Crop Protection, Journal Year: 2025, Volume and Issue: 195, P. 107261 - 107261

Published: May 5, 2025

Language: Английский

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Functional investigation of CYP304F1 in Tuta absoluta (Lepidoptera: Gelechiidae) by RNA interference

Journal of Economic Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Tuta absoluta has developed resistance to many biological insecticides, causing substantial agricultural and economic losses annually. P450s have been the most extensively studied enzymes in context of insecticide metabolism insect pests, detoxification T. against insecticides remains poorly understood. In absoluta, CYP304F1 was screened from comparative transcriptome 2 regional populations Xinjiang, China. The objective present study characterize analyze explore its role spinetoram as well growth development absoluta. Following cloning sequence analysis target gene, it named CYP304F1. Expression levels were then determined after exposure across various developmental instars tissues. Finally, dsCYP304F1 synthesized utilized assess effects post-RNAi on larval susceptibility, growth, development. Sequence revealed that harbors conserved domains characteristic P450 proteins, exhibiting high conservation within Lepidoptera clade. Treatment with an LC50 dose significantly upregulated expression larvae. Silencing enhanced susceptibility prolonged leaf-mining duration time 2nd instar 4th by 40% 17.6%, respectively, compared controls. And feeding dsCYP304F1-treated leaves for 6 days resulted 71% mortality. These results suggested played a crucial

Language: Английский

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Effects of Amino Acid Mutation in Cytochrome P450 (CYP96A146) of Descurainia sophia on the Metabolism and Resistance to Tribenuron-Methyl

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Cytochrome P450 monooxygenases (P450s) play important roles in herbicide resistance. In this study, there are four amino acid mutations (F39Y, H163Y, S203A, and V361E) between

Language: Английский

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Oxidative metabolism mechanism of terpenoid compound ZQ-8 by cytochrome P450 enzyme in Helicoverpa armigera

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138989 - 138989

Published: Dec. 1, 2024

Language: Английский

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