Molecular Mechanism of λ-Cyhalothrin Detoxification by a Delta-Class Glutathione S-Transferase (PxGSTD3) from Plutella xylostella DOI
Xinyu Li,

Zhouyuan Liu,

Xinxin Lv

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

The diamondback moth (Plutella xylostella) exhibits significant resistance to commonly used insecticides including λ-cyhalothrin. Delta-class glutathione S-transferases (GSTs) are crucial detoxification enzymes involved in insecticide and resistance. We demonstrate that PxGSTD3 is associated with the λ-cyhalothrin contributes detoxification. transcription of was rapidly upregulated response exposure, recombinant protein exhibited metabolic activity against Further investigation using computer-aided drug design revealed binding mechanism toward results showed binds an active pocket through noncovalent interactions such as hydrogen bonds, π-π stacking, hydrophobic interactions. Residues Arg36, Tyr115, Phe119 were found have a critical impact on metabolism by PxGSTD3. These findings provide valuable insights into role GST detoxifying offer theoretical guidance for novel pyrethroid-based insecticides.

Language: Английский

GSTD1 Mediates the Tolerance to Abamectin and Beta-Cypermethrin in the Fall Armyworm Spodoptera frugiperda DOI Creative Commons
Qian Ding, Yangyang Liu, Bo Dai

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(3), P. 299 - 299

Published: March 12, 2025

Glutathione S-transferase (GST) is a class of detoxifying enzymes in the second stage insect metabolism and plays key role insecticide resistance. In this study, based on transcriptome sequences S. frugiperda, full-length cDNA SfGSTD1 was cloned characterized. The temporal spatial expression pattern showed that highly expressed Malpighian tubules, which are excretion organs. Knocking down reduced frugiperd tolerance to abamectin beta-cypermethrin. overexpression enhanced viability Sf9 cell under beta-cypermethrin treatment. Furthermore, overexpressed Drosophila melanogaster using GAL4/UAS binary system, strain also less susceptible enzyme activity recombinant could be significantly inhibited by Taken together, our findings indicate might involved frugiperda. And these results provide theoretical foundations for understanding resistance mechanism frugiperda

Language: Английский

Citations

0
A symbiotic bacterium regulates the detoxification metabolism of deltamethrin in Aedes albopictus DOI Creative Commons

Si‐Jia Deng,

Lei Tu, Lin Li

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106445 - 106445

Published: May 1, 2025

Language: Английский

Citations

0
Molecular Mechanism of λ-Cyhalothrin Detoxification by a Delta-Class Glutathione S-Transferase (PxGSTD3) from Plutella xylostella DOI
Xinyu Li,

Zhouyuan Liu,

Xinxin Lv

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

The diamondback moth (Plutella xylostella) exhibits significant resistance to commonly used insecticides including λ-cyhalothrin. Delta-class glutathione S-transferases (GSTs) are crucial detoxification enzymes involved in insecticide and resistance. We demonstrate that PxGSTD3 is associated with the λ-cyhalothrin contributes detoxification. transcription of was rapidly upregulated response exposure, recombinant protein exhibited metabolic activity against Further investigation using computer-aided drug design revealed binding mechanism toward results showed binds an active pocket through noncovalent interactions such as hydrogen bonds, π-π stacking, hydrophobic interactions. Residues Arg36, Tyr115, Phe119 were found have a critical impact on metabolism by PxGSTD3. These findings provide valuable insights into role GST detoxifying offer theoretical guidance for novel pyrethroid-based insecticides.

Language: Английский

Citations

0