Exosome-like nanoparticles derived from fruits, vegetables, and herbs: innovative strategies of therapeutic and drug delivery

Theranostics, Journal Year: 2024, Volume and Issue: 14(12), P. 4598 - 4621

Published: Jan. 1, 2024

Over the past ten years, significant advancements have been made in exploring plant-derived exosome-like nanoparticles (PELNs) for disease therapeutics and drug delivery. PELNs, as inherent nanoscale particles comprised of proteins, lipids, nucleic acids, secondary metabolites, exhibit capacity cellular uptake by human cells. This intercellular interaction transcends biological boundaries, effectively influencing functions animals. PELNs outstanding biocompatibility, low immunogenicity, enhanced safety, environmentally friendly sustainability. article summarized preparation methods characteristics PELNs. It provided a systematic review varied roles derived from fruits, vegetables, herbs The challenges their production application were discussed, future prospects this rapidly evolving field explored.

Language: Английский

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Exosomes in Autoimmune Diseases: A Review of Mechanisms and Diagnostic Applications

Clinical Reviews in Allergy & Immunology, Journal Year: 2025, Volume and Issue: 68(1)

Published: Jan. 17, 2025

Language: Английский

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Advancing functional foods: a systematic analysis of plant-derived exosome-like nanoparticles and their health-promoting properties

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: March 5, 2025

Plant-derived exosome-like nanoparticles (PDENs), emerging as novel bioactive agents, exhibit significant potential in food science and nutritional health. These nanoparticles, enriched with plant-specific biomolecules such proteins, lipids, nucleic acids, secondary metabolites, demonstrate unique cross-species regulatory capabilities, enabling interactions mammalian cells gut microbiota. PDENs enhance nutrient bioavailability by protecting sensitive compounds during digestion, modulate metabolic pathways through miRNA-mediated gene regulation, anti-inflammatory antioxidant properties. For instance, grape-derived reduce plasma triglycerides high-fat diets, while ginger-derived alleviate colitis downregulating pro-inflammatory cytokines. Additionally, serve natural drug carriers, applications delivering therapeutic agents like doxorubicin paclitaxel. Despite these advancements, challenges remain standardizing extraction methods (ultracentrifugation, immunoaffinity), ensuring stability processing storage, evaluating long-term safety. Current research highlights the need for optimizing lyophilization techniques understanding between matrices. Furthermore, show promise functional development—such fortified beverages probiotic formulations—their clinical translation requires rigorous pharmacokinetic studies clarity. This review synthesizes existing knowledge on PDENs’ composition, biological activities, applications, identifying gaps scalability, stability, safety assessments. Future directions emphasize interdisciplinary collaboration to harness combating disorders, enhancing functionality, advancing personalized nutrition strategies.

Language: Английский

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From Plant Based Therapy to Plant-Derived Vesicle-Like Nanoparticles for Cancer Treatment: Past, Present and Future

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 3471 - 3491

Published: March 1, 2025

Cancer stands as a formidable malady profoundly impacting human health. Throughout history, plant-based therapies have remained pivotal in the arsenal against cancer, evolving alongside epochs. Presently, challenges such arduous extraction of active components and potential safety concerns impede progression anticancer therapies. The isolation plant-derived vesicle-like nanoparticles (PDVLNs), kind lipid bilayer capsules isolated from plants, has brought therapy into novel realm led to decades research on PDVLNs. Accumulating evidence indicates that PDVLNs can deliver substances cells regulate cellular functions. Regulating immunity, inducing cell cycle arrest, promoting apoptosis cancer are most commonly reported mechanisms tumor suppression. Low immunogenicity lack tumorigenicity make good platform for drug delivery. molecules within all source so selection plants is crucial. In recent years, there been clear trend changed vegetables or fruits medicinal plants. This review highlights identify candidate More importantly, current PDVLN-based applications delivery systematically discussed.

Language: Английский

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Colon health benefits of plant-derived exosome-like nanoparticles via modulating gut microbiota and immunity

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 21

Published: March 19, 2025

Exosomes are nanoscale particles with a lipid bilayer membrane that were first identified in mammalian cells. Plant-derived exosome-like nanoparticles (PELNs) share structural and molecular similarities exosomes, including lipids, proteins, microRNA (miRNA), plant-derived metabolites. Owing to their unique characteristics, such as outstanding stability, low immunogenicity, high biocompatibility, sustainability, PELNs have emerged promising natural bioactive agents the capacity for cross-kingdom cellular regulation. Dietary supplementation PELNs, particularly from fruits vegetables, has demonstrated health benefits. An increasing number of studies indicated beneficial effects on colon health. This review summarizes isolation characterization uptake, distribution after oral ingestion. Furthermore, this emphasizes interactions between gut microbiota, immune system, uptake by modulation bacteria metabolism, responses PELNs. Additionally, applications components drug carriers targeting reviewed. In summary, represent versatile approach improve health, potential both therapeutic preventive healthcare strategies.

Language: Английский

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Plant-derived exosome-like nanoparticles for microRNA delivery in cancer treatment

Drug Delivery and Translational Research, Journal Year: 2024, Volume and Issue: unknown

Published: May 17, 2024

Language: Английский

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A Systematic Review on Plant-Derived Extracellular Vesicles as Drug Delivery Systems

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7559 - 7559

Published: July 10, 2024

This systematic review offers a comprehensive analysis of plant-derived extracellular vesicles (PDEVs) as emerging drug delivery systems, focusing on original research articles published between 2016 and 2024 that exclusively examine the use PDEVs for delivery. After rigorous search across multiple databases, 20 relevant studies out 805 initial results were selected analysis. systematically summarizes critical data PDEV components, isolation methods, drug-loading techniques. It highlights potential to significantly enhance safety efficacy, reduce dosage toxicity, align development with sustainable environmentally friendly biotechnological processes. also emphasizes advantages over mammalian-derived vesicles, such cost-effectiveness, higher yield, reduced immunogenicity. Additionally, it explores synergistic encapsulated drugs bioactive compounds naturally present in PDEVs. study acknowledges challenges standardizing formulation methods clinical use. Overall, this provides valuable insights into current state future directions PDEV-based highlighting their promising role advancing pharmaceutical development.

Language: Английский

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Cucumaria frondosa intestines and ovum hydrolysates intervention ameliorates the symptoms of dextran sulfate sodium‐induced colitis by modulating gut microbiota and its metabolites

Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(3)

Published: March 1, 2025

Colitis, a troublesome inflammatory disease that significantly impacts daily life, has garnered considerable attention in recent times. Protolysates play crucial role the treatment of colitis, and intestines ovum Cucumaria frondosa represent readily available source these hydrolysates. However, effects C. hydrolysates (CFHs) on colitis have not been thoroughly investigated. We initially examined molecular weight distribution CFHs found fraction molecules with less than 1000 Da accounted for 86.98%, indicating hydrolysis primarily produced oligopeptides. Subsequently, we employed dextran sulfate sodium-induced experimental model to assess therapeutic potential CFHs. The findings indicated preventive administration dramatically attenuated pathological manifestations associated mice, including loss, colon shortening, tissue damage. Furthermore, suppressed secretion pro-inflammatory cytokines IL-6, TNF-α, IL-1β, as well MPO tissue. Metagenomic sequencing demonstrated could restore balance dysregulated gut microbiota by reinforcing Bacteroidota suppressing Verrucomicrobia populations, impacting various microbial functions. Metabolomic analyses further revealed exhibited more efficacious modulatory effect DSS-induced metabolic abnormalities, amino acid biosynthesis, linoleic metabolism, dopaminergic synapses. In conclusion, showed promise alleviating laying groundwork development application functional food relief.

Language: Английский

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Cutting-edge insights into the mechanistic understanding of plant-derived exosome-like nanoparticles: Implications for intestinal homeostasis

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116186 - 116186

Published: March 1, 2025

Language: Английский

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Garlic-Derived Exosome-Like Nanoparticles Enhance Gut Homeostasis in Stressed Piglets: Involvement of Lactobacillus reuteri Modulation and Indole-3-propionic Acid Induction

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

The occurrence of pediatric diarrhea is frequently associated with inflammatory responses, compromised barrier function, and dysbiosis in the gut. These conditions are commonly triggered by stressors, similar to postweaning observed piglets. Garlic-derived exosome-like nanoparticles (GELNs) hold potential for ameliorating stress-induced diarrhea, yet supporting evidence remains scarce. Following successful isolation GELNs, this study employed weaned piglets as a model evaluate regulatory effects GELNs on intestinal integrity, mucosal inflammation, gut microbiota its metabolites. Weaned Bama miniature were orally administered phosphate buffer saline (PBS) or 1 week later, samples collected following slaughter. Histological molecular biological techniques performed examine structure, tight junction protein expression, mucin secretion, T lymphocyte infiltration, levels pro-inflammatory cytokines. composition was analyzed using 16S rRNA sequencing, while derived metabolites profiled via untargeted metabolomics. Subsequently, correlation analyses associations between metabolites, well key indicators function cytokine response GELNs. isolated exhibit typical exosome characteristics size morphology, alongside rich content proteins RNAs. incidence reduced supplementation at dosage 50 mg/kg body weight, compared control group. In addition, receiving displayed an increase within tissues jejunum, ileum, colon, decrease CD8+ counts suppression cytokines (IL-8 TNF-α) layers both jejunum ileum. Furthermore, sequencing unveiled that reshaped colonic augmenting beneficial bacteria, notably Lactobacillus reuteri, correlating strongly diminished TNF-α heightened expression. Metabolite analysis demonstrated significant indole-3-propionic acid, from microbiota, supplemented This positively correlated abundance reuteri negatively linked IL-8 summary, our demonstrates mitigate stress-related inflammation enhance production piglets, which potentially achieved through optimization composition, specifically increasing induction anti-inflammatory microbial metabolite acid. findings presented here provide essential groundwork future development therapeutic strategy aimed enhancing homeostasis disruption caused stress children.

Language: Английский

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