The Mechanism of Propofol in Treating Depression-like Behaviors Based on Network Pharmacology and Experimental Validation DOI Creative Commons
Jiarong Wang,

J. Z. Xiang,

Xuemei Han

et al.

International Journal of Biology and Life Sciences, Journal Year: 2024, Volume and Issue: 8(1), P. 54 - 59

Published: Nov. 22, 2024

Objective: To explore the potential mechanism of propofol in improving antidepressant-like behavior through network pharmacology and animal experimental validation, providing theoretical support for development novel antidepressant drugs based on propofol. Methods: 1. The targets disease depression were screened Swiss Target prediction, Super-pred, SEA, Drugbank, CTD, GeneCards, OMIM, TTD databases. A protein-protein interaction (PPI) was constructed, core visualized using CytoScape 3.10.2 software. Additionally, GO KEGG enrichment analyses intersection performed Metascape database. 2. Thirty male C57BL/6 mice randomly divided into CON group, LH model PRO treatment with 10 each group. sucrose preference test, forced swim tail suspension test conducted to determine whether successfully established assess improvement effect behavior. 3. pathological morphological changes hippocampal neurons three groups observed HE staining experiments. expression mGluR5 protein hippocampus analyzed by Western blot. Results: Network analysis indicated that may exert effects acting genes such as ALB, ESR1, NFKB1, HSP90AB1, EGFR. These target mainly enriched biological processes synaptic transmission membrane regulation; they involved cellular components GABA receptor complexes membranes; included molecular functions neurotransmitter activity. signaling pathways neuroactive ligand-receptor interaction, morphine addiction, GABAergic synapse. Compared rate group significantly decreased, while immobility time increased. increased, decreased. results showed compared number loose arrangement, pycnotic deeply stained nuclei blurred boundaries. However, necrotic blot level it decreased Conclusion: Propofol regulating mGluR5, behavior, reducing neuronal necrosis.

Language: Английский

Role of Glial Cells in Neuronal Function, Mood Disorders, and Drug Addiction DOI Creative Commons
Yousef Tizabi, Bruk Getachew, Sheketha R. Hauser

et al.

Brain Sciences, Journal Year: 2024, Volume and Issue: 14(6), P. 558 - 558

Published: May 30, 2024

Mood disorders and substance use disorder (SUD) are of immense medical social concern. Although significant progress on neuronal involvement in mood reward circuitries has been achieved, it is only relatively recently that the role glia these attracted attention. Detailed understanding glial functions devastating diseases could offer novel interventions. Here, following a brief review involved regulation perception, specific contributions neurotrophic factors, neuroinflammation, gut microbiota to highlighted. In this context, cells (e.g., microglia, astroglia, oligodendrocytes, synantocytes) phenotypic manifestation or SUD emphasized. addition, knowledge potential development therapeutics touched upon.

Language: Английский

Citations

13
Esketamine-mediated alleviation of electroconvulsive shock-induced memory impairment is associated with the regulation of mGluR5 in depressive-like rats DOI Creative Commons
Yiwei Shen, Ran Wei, Dawei Liu

et al.

Pharmacology Biochemistry and Behavior, Journal Year: 2025, Volume and Issue: 248, P. 173955 - 173955

Published: Jan. 4, 2025

Language: Английский

Citations

0
Elucidating genetic and molecular basis of altered higher-order brain structure-function coupling in major depressive disorder DOI Creative Commons
Haixia Long, Zihao Chen,

Xinli Xu

et al.

NeuroImage, Journal Year: 2024, Volume and Issue: 297, P. 120722 - 120722

Published: July 4, 2024

Previous studies have shown that major depressive disorder (MDD) patients exhibit structural and functional impairments, but few investigated changes in higher-order coupling between structure function. Here, we systematically the effect of MDD on connectivity (SC) (FC). Each brain region was mapped into embedding vector by node2vec algorithm. We used support machine (SVM) with to distinguish from health controls (HCs) identify most discriminative regions. Our study revealed had decreased connections regions local rich-club organization increased ventral attentional network limbic compared HCs. Interestingly, transcriptome-neuroimaging association analysis demonstrated correlations regional rSC-FC variations HCs α/β-hydrolase domain-containing 6 (ABHD6), β 1,3-N-acetylglucosaminyltransferase-9(β3GNT9), transmembrane protein 45B (TMEM45B), correlation dSC-FC retinoic acid early transcript 1E antisense RNA 1(RAET1E-AS1), iSC-FC ABHD6, β3GNT9, katanin-like 2 (KATNAL2). In addition, neurotransmitter receptor/transporter maps found were both correlated neuroendocrine transporter (NET) expression, metabotropic glutamate receptor 5 (mGluR5). Further mediation explored relationship genes, related variations. These findings indicate specific genetic molecular factors underpin observed disparities SC-FC confirmed SC FC plays an important role diagnosing MDD. The identification new biological evidence for etiology holds promise development innovative antidepressant therapies.

Language: Английский

Citations

3
Role of Glial Cells in Depression and Drug Addiction DOI Open Access
Yousef Tizabi, Bruk Getachew, Sheketha R. Hauser

et al.

Published: May 3, 2024

Major depressive disorder (MDD) and substance use (SUD) are of immense medical social concerns. Although significant progress on neuronal involvement in mood reward circuitries has been achieved, only relatively recently the role glia these disorders have attracted attention. Detail understanding glial function devastating diseases could offer novel interventions. Here, following a brief review involved regulation perception, specific contribution neurotrophic factors, neuroinflammation gut microbiota to highlighted. In this context, cells (e.g., microglia, astroglia, oligodendrocytes synantocytes) phenotypic manifestation MDD or SUD emphasized. addition, potential knowledge developing therapeutics is touched upon.

Language: Английский

Citations

1
The Mechanism of Propofol in Treating Depression-like Behaviors Based on Network Pharmacology and Experimental Validation DOI Creative Commons
Jiarong Wang,

J. Z. Xiang,

Xuemei Han

et al.

International Journal of Biology and Life Sciences, Journal Year: 2024, Volume and Issue: 8(1), P. 54 - 59

Published: Nov. 22, 2024

Objective: To explore the potential mechanism of propofol in improving antidepressant-like behavior through network pharmacology and animal experimental validation, providing theoretical support for development novel antidepressant drugs based on propofol. Methods: 1. The targets disease depression were screened Swiss Target prediction, Super-pred, SEA, Drugbank, CTD, GeneCards, OMIM, TTD databases. A protein-protein interaction (PPI) was constructed, core visualized using CytoScape 3.10.2 software. Additionally, GO KEGG enrichment analyses intersection performed Metascape database. 2. Thirty male C57BL/6 mice randomly divided into CON group, LH model PRO treatment with 10 each group. sucrose preference test, forced swim tail suspension test conducted to determine whether successfully established assess improvement effect behavior. 3. pathological morphological changes hippocampal neurons three groups observed HE staining experiments. expression mGluR5 protein hippocampus analyzed by Western blot. Results: Network analysis indicated that may exert effects acting genes such as ALB, ESR1, NFKB1, HSP90AB1, EGFR. These target mainly enriched biological processes synaptic transmission membrane regulation; they involved cellular components GABA receptor complexes membranes; included molecular functions neurotransmitter activity. signaling pathways neuroactive ligand-receptor interaction, morphine addiction, GABAergic synapse. Compared rate group significantly decreased, while immobility time increased. increased, decreased. results showed compared number loose arrangement, pycnotic deeply stained nuclei blurred boundaries. However, necrotic blot level it decreased Conclusion: Propofol regulating mGluR5, behavior, reducing neuronal necrosis.

Language: Английский

Citations

0