Engineering Strategies of Plant-Derived Exosome-Like Nanovesicles: Current Knowledge and Future Perspectives DOI Creative Commons
Yuhan Li, Yulong Wang,

Hongrui Zhao

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 12793 - 12815

Published: Nov. 1, 2024

Plant-derived exosome-like nanovesicles (PELNs) from edible plants, isolated by ultracentrifugation, size exclusion chromatography or other methods, were proved to contain a variety of biologically active and therapeutically specific components. Recently, investigations in the field PELN-based biomedicine have been conducted, which positioned those as promising tools for prevention treatment several diseases, with their natural origin potentially offering superior biocompatibility bioavailability. However, inadequate targeting limited therapeutic effects constrain utility clinical translation PELNs. Thus, strategies aiming at bridging gap engineering PELNs great interest. Those approaches include membrane hybridization, physical chemical surface functionalization encapsulation payloads. Herein, we provide comprehensive overview biogenesis composition, isolation purification methods characterization PELNs, well functions. Current knowledge on construction biomedical application engineered reviewed. Additionally, future directions perspectives this discussed order further enrich expand prospects

Language: Английский

Plant-Derived Extracellular Vesicles: Natural Nanocarriers for Biotechnological Drugs DOI Open Access
Eleonora Calzoni, Agnese Bertoldi, Gaia Cusumano

et al.

Processes, Journal Year: 2024, Volume and Issue: 12(12), P. 2938 - 2938

Published: Dec. 23, 2024

Plant-derived extracellular vesicles (PDEVs) are lipid bilayer nanoparticles, naturally produced by plant cells, with sizes ranging from 50 to 500 nm. Recent studies have highlighted their great potential in the biotechnological and medical fields, due natural origin, high biocompatibility intrinsic therapeutic properties. PDEVs contain a complex biological cargo of proteins, lipids, nucleic acids secondary metabolites, including antioxidants anti-inflammatory molecules, making them ideal for biomedical applications such as drug delivery. These play key role intercellular communication gene regulation, proving be particularly promising personalized medicine. ability improve stability bioavailability, optimizing targeted release minimizing side effects. Despite some challenges, compositional variability need standardized protocols, at gunsight innovative research aimed improving loading capacity specificity. This review aims provide comprehensive overview PDEVs, exploring structure, isolation methods, functional characteristics, applications, highlighting advantages over synthetic nanoparticles animal-derived vesicles, leading an sustainable solution development new approaches.

Language: Английский

Citations

4
Nature Herbal Medicine‐ Tissue Engineering Strategies for Regulate Cell Homeostasis in Bone Regeneration DOI Open Access
Xu Wei, Hao Xie, Can Liu

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Abstract Bone fractures, especially large and complex defects, continue to pose significant challenges in the medical field. Current treatments often rely on autografts or allografts for structural support, which can lead problems such as reduced bioactivity, infection risks, potential pathogen transmission. Nature herbal medicine (NHM), including herbs extracts, offers a promising alternative by effectively modulating inflammatory responses, enhancing osteoblast function, inhibiting bone resorption, thereby facilitating fracture repair. However, traditional drug delivery methods NHM encounter interactions, poor tissue distribution, patient compliance. Biomaterials, engineered interact with biological systems, play essential roles repair, mechanical delivery. When used carriers, biomaterials be combined form stable systems that further promote regeneration. Here applications of are reviewed, hydrogels, extracellular vesicles, cement, conjunction regulating homeostasis aiming provide valuable insights guidance future research therapeutic strategies.

Language: Английский

Citations

0
Mesenchymal Stem Cell–Derived Exosome Efficacy and Safety in Musculoskeletal Tissues: State of The Art and Future Directions DOI
Nasim Eshraghi, Amin Javidan,

Neil Nazar Al-Saeidi

et al.

Regenerative Engineering and Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Language: Английский

Citations

0
Exosome-like Nanoparticles, High in Trans-δ-Viniferin Derivatives, Produced from Grape Cell Cultures: Preparation, Characterization, and Anticancer Properties DOI Creative Commons
Yuri N. Shkryl, Zhargalma Tsydeneshieva, Ekaterina S. Menchinskaya

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 2142 - 2142

Published: Sept. 20, 2024

Background: Recent interest in plant-derived exosome-like nanoparticles (ENs) has surged due to their therapeutic potential, which includes antioxidant, anti-inflammatory, and anticancer activities. These properties are attributed cargo of bioactive metabolites other endogenous molecules. However, the ENs isolated from plant cell cultures remain less explored. Methods: In this investigation, grape callus-derived (GCENs) were using differential ultracentrifugation techniques. Structural analysis through electron microscopy, nanoparticle tracking analysis, western blotting confirmed that GCENs qualify as nanovesicles. Results: contained significant amounts microRNAs proteins characteristic ENs, well trans-δ-viniferin, a notable stilbenoid known for its health-promoting properties. Functional assays revealed reduced viability triple-negative breast cancer line MDA-MB-231 dose-dependent manner. Moreover, exhibited negligible effects on normal human embryonic kidney (HEK) 293 cells, indicating selective cytotoxicity. Notably, treatment with these led cycle arrest G1 phase triggered apoptosis line. Conclusions: Overall, study underscores potential nanovectors natural carriers stilbenoids proposes application novel effective approach management cancer.

Language: Английский

Citations

3
New Nanovesicles from Prickly Pear Fruit Juice: A Resource with Antioxidant, Anti-Inflammatory, and Nutrigenomic Properties DOI Creative Commons
Flores Naselli, Sara Volpes, Paola Sofia Cardinale

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(21), P. 1756 - 1756

Published: Oct. 23, 2024

Plant-derived nanovesicles represent a novel approach in the field of plant-derived biomaterials, offering sustainable and biocompatible option for various biomedical applications. The unique properties these vesicles, such as their ability to encapsulate bioactive compounds, make them suitable therapeutic, cosmetic, nutraceutical purposes. In this study, we have, first time, successfully bio-fabricated vesicles derived from Opuntia ficus-indica (FicoVes) using an efficient cost-effective method. Characterized by size approximately 114 nm negative zeta potential -20.9 mV, FicoVes exhibited excellent biocompatibility hemocompatibility, showing no reduction viability human animal cells. Our results showed that possess significant antioxidant they reduced ROS generation TBH-stimulated displayed anti-inflammatory reducing expression pro-inflammatory cytokines (Il 1β, TNF α) enhancing (IL4, IL10) following inflammatory stimulus. Furthermore, accelerated epithelial wound closure L929 fibroblast monolayers dose-dependent manner, highlighting role tissue repair. This study establishes promising candidate nutrigenomic applications, particularly context inflammation-related disorders healing. Further research, including vivo studies, is essential validate findings fully explore therapeutic potential.

Language: Английский

Citations

1
Chinese herbal medicine-derived extracellular vesicles as novel biotherapeutic tools: present and future DOI Creative Commons
Jinying Zhang, Shuo Tian, Lin Guo

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 25, 2024

Extracellular vesicles (EVs) are phospholipid bilayer-enclosed biological particles that secreted by almost all living cells including animals, plants, and microorganisms. Chinese herbal medicines (CHM) have a long history of using plant-based remedies to treat prevent human diseases. medicine-derived extracellular vesicle (CHMEV) generic term refers nanoscale membrane structures isolated from medicinal plants such as ginseng, ginger, Panax notoginseng. In recent years, CHMEVs garnered substantial attention novel class functional components due their high bioavailability, safety, easy accessibility, diverse therapeutic effects, indicating great potential for development new dosage form CHM. Research on in traditional medicine (TCM) has become prominent area interest, opening avenues further exploration into the effects mechanisms Nonetheless, an emerging field, there is much unknown about these vesicles, current research remains inconsistent. The review comprehensively summarizes biogenesis, isolation methods, physical, biochemical characterizations CHMEVs. Additionally, we highlight biomedical applications agents drug delivery carriers, anti-inflammatory, anticancer, regenerative, antiaging activities. Finally, propose challenges future perspectives. By summarizing existing literature, aim offer valuable clues inspiration CHMEV research, thereby facilitating standardization treatment diseases discovery.

Language: Английский

Citations

1
Engineering Strategies of Plant-Derived Exosome-Like Nanovesicles: Current Knowledge and Future Perspectives DOI Creative Commons
Yuhan Li, Yulong Wang,

Hongrui Zhao

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 12793 - 12815

Published: Nov. 1, 2024

Plant-derived exosome-like nanovesicles (PELNs) from edible plants, isolated by ultracentrifugation, size exclusion chromatography or other methods, were proved to contain a variety of biologically active and therapeutically specific components. Recently, investigations in the field PELN-based biomedicine have been conducted, which positioned those as promising tools for prevention treatment several diseases, with their natural origin potentially offering superior biocompatibility bioavailability. However, inadequate targeting limited therapeutic effects constrain utility clinical translation PELNs. Thus, strategies aiming at bridging gap engineering PELNs great interest. Those approaches include membrane hybridization, physical chemical surface functionalization encapsulation payloads. Herein, we provide comprehensive overview biogenesis composition, isolation purification methods characterization PELNs, well functions. Current knowledge on construction biomedical application engineered reviewed. Additionally, future directions perspectives this discussed order further enrich expand prospects

Language: Английский

Citations

1