Metagenomics and Non‐Targeted Metabolomics Reveal the Role of Gut Microbiota and Its Metabolites in Brain Metastasis of Non‐Small Cell Lung Cancer DOI Creative Commons
Chen‐Guang Liu,

Mei‐Xi Lin,

Yu Xin

et al.

Thoracic Cancer, Journal Year: 2025, Volume and Issue: 16(8)

Published: April 1, 2025

ABSTRACT Background Brain metastasis is a common and severe complication in non‐small cell lung cancer (NSCLC) patients, significantly affecting prognosis. However, the role of gut microbiota its metabolites NSCLC brain remains poorly understood. This study aims to explore relationship between microbiota, metabolites, development NSCLC. Methods We conducted an integrative analysis combining metagenomics non‐targeted metabolomics on baseline fecal samples from patients with ( n = 18) those without distant 12). Gut composition metabolite profiles were detected analyzed, statistical methods, including machine learning models, applied identify differences potential biomarkers. Results Significant found two groups, higher microbial diversity observed metastasis. Specific genera, such as Paenibacillus , Fournierella Adlercreutzia enriched group. Metabolomic revealed altered levels short‐chain fatty acids other associated immune modulation vascular permeability, angiotensin (1–7). These changes linked metastatic process may influence development. Furthermore, models identified key biomarkers, Raoultibacter Mobilibacterium N ‐acetyl‐L‐glutamic acid, which could serve valuable indicators for Conclusions Our findings suggest that dysbiosis metabolic products contribute The identification microbiota‐derived biomarkers holds early detection therapeutic intervention

Language: Английский

Impact of Metabolites from Foodborne Pathogens on Cancer DOI Creative Commons
Alice Njolke Mafe, Dietrich Büsselberg

Foods, Journal Year: 2024, Volume and Issue: 13(23), P. 3886 - 3886

Published: Dec. 1, 2024

Foodborne pathogens are microorganisms that cause illness through contamination, presenting significant risks to public health and food safety. This review explores the metabolites produced by these pathogens, including toxins secondary metabolites, their implications for human health, particularly concerning cancer risk. We examine various such as Salmonella sp., Campylobacter Escherichia coli, Listeria monocytogenes, detailing specific of concern carcinogenic mechanisms. study discusses analytical techniques detecting chromatography, spectrometry, immunoassays, along with challenges associated detection. covers effective control strategies, processing techniques, sanitation practices, regulatory measures, emerging technologies in pathogen control. manuscript considers broader highlighting importance robust policies, awareness, education. identifies research gaps innovative approaches, recommending advancements detection methods, preventive policy improvements better manage foodborne metabolites.

Language: Английский

Citations

11
Exploring the mediating role of blood metabolites in the relationship between gut microbiota and gastric cancer risk: a Mendelian randomization study DOI Creative Commons
Xiaocheng Li, Huapeng Lin, Jing Peng

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 7, 2025

Prior studies have established correlations between gut microbiota (GM) dysbiosis, circulating metabolite alterations, and gastric cancer (GC) risk. However, the causal nature of these associations remains uncertain. We utilized summary data from genome-wide association (GWAS) on GM (European, n=8,956), blood metabolites n=120,241; East Asian, n=4,435), GC n=476,116; n=167,122) to perform a bidirectional Mendelian randomization (MR) analysis, investigating effects Additionally, we conducted mediation analysis (two-step MR) identify potential mediators in GM-GC relationship. identified twelve negative seven positive specific taxa For metabolites, traits were found be significantly associated with reduced risk European population, findings subsequently validated Asian cohort. Three showed five metabolic traits: Bacteroidia class Bacteroidales order positively correlated (all P < 0.013), while Bacteroides OTU97_27 exhibited correlation one (P = 0.007). Two-step MR indicated that total lipids intermediate-density lipoprotein (IDL), IDL particle concentration, phospholipids medium low-density (LDL), small LDL, free cholesterol LDL indirectly influenced class/Bacteroidales GC, proportions 1.71% 0.048), 1.69% 2.05% 0.045), 1.85% 1.99% respectively. The present study provides suggestive evidence relationship GM, risk, potentially offering new insights into etiology.

Language: Английский

Citations

0
Unveiling the Interplay Between the Human Microbiome and Gastric Cancer: A Review of the Complex Relationships and Therapeutic Avenues DOI Open Access
Jenan Al‐Matouq,

Hawra Al-Ghafli,

Noura N. Alibrahim

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(2), P. 226 - 226

Published: Jan. 12, 2025

The human microbiota plays a crucial role in maintaining overall health and well-being. gut has been implicated developing progressing various diseases, including cancer. This review highlights the related mechanisms compositions that influence cancer pathogenesis with highlight on gastric We provide comprehensive overview of by which microbiome influences development, progression, response to treatment, focus identifying potential biomarkers for early detection, prevention strategies, novel therapeutic interventions leverage modulation. can guide future research clinical practices understanding harnessing optimize therapies.

Language: Английский

Citations

0
Transcriptional and microbial profile of gastric cancer patients infected with Epstein-Barr virus DOI Creative Commons

Klezzer de Oliveira Carneiro,

Taíssa Maíra Thomaz Araújo, Ronald Matheus da Silva Mourão

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: March 5, 2025

Gastric cancer (GC), which has low survival rates and high mortality, is a major concern, particularly in Asia South America, with over one million annual cases. Epstein-Barr virus (EBV) recognized as carcinogen that may trigger gastric carcinogenesis by infecting the stomach epithelium via reactivated B cells, growing evidence linking it to GC. This study investigates transcriptional microbial profiles of EBV-infected versus EBV-non-infected GC patients. Using Illumina NextSeq, cDNA libraries were sequenced, reads aligned human genome analyzed DESeq2. Kegg differential analyses revealed key genes pathways. Gene sensitivity specificity assessed using ROC curves (p < 0.05, AUC > 0.8). Non-aligned used for microbiome analysis Kraken2 bacterial identification. Microbial included LDA score, Alpha Beta diversity metrics, significance set at p ≤ 0.05. Spearman's correlation between differentially expressed (DEGs) bacteria also examined. The data gene expression pattern EBV-positive cancer, highlighting immune response, inflammation, cell proliferation (e.g., GBP4, ICAM1, IL32, TNFSF10). identified discriminating EBV+ including GBP5, CMKLR1, GM2A CXCL11 play pivotal roles cancer. Functional enrichment pointed cytokine-cytokine receptor interactions, antigen processing, Th17 emphasizing role tumor microenvironment, shaped inflammation immunomodulation, EBV-associated changes microbiota samples, significant reduction taxa. genera Choristoneura Bartonella more abundant GC, while EBV- Citrobacter, Acidithiobacillus Biochmannia. showed strong link DE DEGs involved processes like differentiation, cytokine production, digestion, death. These findings suggest complex interaction host (EBV+ GC) microbiota, possibly influencing progression, offering potential therapeutic targets such modulation or regulation. Comparing samples further highlights specific impact EBV on pathogenesis.

Language: Английский

Citations

0
Metagenomics and Non‐Targeted Metabolomics Reveal the Role of Gut Microbiota and Its Metabolites in Brain Metastasis of Non‐Small Cell Lung Cancer DOI Creative Commons
Chen‐Guang Liu,

Mei‐Xi Lin,

Yu Xin

et al.

Thoracic Cancer, Journal Year: 2025, Volume and Issue: 16(8)

Published: April 1, 2025

ABSTRACT Background Brain metastasis is a common and severe complication in non‐small cell lung cancer (NSCLC) patients, significantly affecting prognosis. However, the role of gut microbiota its metabolites NSCLC brain remains poorly understood. This study aims to explore relationship between microbiota, metabolites, development NSCLC. Methods We conducted an integrative analysis combining metagenomics non‐targeted metabolomics on baseline fecal samples from patients with ( n = 18) those without distant 12). Gut composition metabolite profiles were detected analyzed, statistical methods, including machine learning models, applied identify differences potential biomarkers. Results Significant found two groups, higher microbial diversity observed metastasis. Specific genera, such as Paenibacillus , Fournierella Adlercreutzia enriched group. Metabolomic revealed altered levels short‐chain fatty acids other associated immune modulation vascular permeability, angiotensin (1–7). These changes linked metastatic process may influence development. Furthermore, models identified key biomarkers, Raoultibacter Mobilibacterium N ‐acetyl‐L‐glutamic acid, which could serve valuable indicators for Conclusions Our findings suggest that dysbiosis metabolic products contribute The identification microbiota‐derived biomarkers holds early detection therapeutic intervention

Language: Английский

Citations

0