Simultaneous Elimination of Reactive Oxygen Species and Activation of Nrf2 by Ultrasmall Nanoparticles to Relieve Acute Kidney Injury

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(13), P. 16460 - 16470

Published: March 22, 2023

Excess reactive oxygen species (ROS) can induce serious acute kidney injury (AKI) to result in numerous deaths annually clinical practice. Elimination of excess ROS by advanced nanotechnology is a very promising AKI therapy. In this Article, we report that PVP-stabilized and quercetin-functionalized ultrasmall Cu2-xSe nanoparticles (abbreviated as CSPQ NPs) efficiently scavenge increase the expression intracellular antioxidative enzymes activating nuclear factor erythroid 2-related 2 (Nrf2) protein, which drastically alleviates cellular oxidative stress. Our exhibit excellent biocompatibility. They be rapidly accumulated into injured simultaneously eliminate activate Nrf2 improve renal function. This work demonstrates great potential simultaneous elimination activation treatment AKI. It also highlights NPs protection prevention

Language: Английский

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Efficacy of traditional Chinese medicine Cordyceps sinensis as an adjunctive treatment in patients with renal dysfunction: a systematic-review and meta-analysis

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 7, 2025

The effectiveness of using Cordyceps sinensis as an adjuvant therapy for patients with renal dysfunction (RD), especially acute kidney injury (AKI), is still a topic debate. In response to the current conflicting data, present meta-analysis was conducted assess clinical C. in treatment RD and provide evidence practice. Several databases, including PubMed, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI) Wanfang, were systematically searched updated March 25, 2024. We used combined ratio (OR) diagnostic (DOR) therapeutic effect sinensis. addition, risk bias assessed by Risk Bias Assessment Tool. ultimately incorporated 15 studies comprising total 1,310 RD. pooled estimated sensitivity, specificity well DOR from patient-based analyses 0.89 (95% confidence interval [CI]: 0.84-0.93), 0.69 CI: 0.59-0.77) 18.0 8.0-39.0), respectively. Moreover, we calculated positive likelihood (PLR) negative (NLR) be 2.8 2.1-3.9) 0.16 0.10-0.27), Additionally, area under curve (AUC) summary receiver operating characteristic (SROC) 0.88 0.85-0.90) reflecting prognostic accuracy. Subsequently, subgroup analysis indicated that efficacy northern Chinese superior southern. On other hand, significantly reduced patients' blood creatinine levels, shortened oliguria period, increased urine osmolality, indicating it can improve function glomeruli tubules. Our results indicate considered dependable individuals It may tubules, promote recovery tubular function, thus enhance effects. www.crd.york.ac.uk/PROSPERO/#recordDetails, identifier CRD42024559042.

Language: Английский

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NfκBin: A Machine Learning based Method for Screening Nf-κB Inhibitors

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Abstract Nuclear Factor kappa B (NF-κB) is a transcription factor whose upregulation associated in chronic inflammatory diseases, including rheumatoid arthritis, bowel disease, and asthma. In order to develop therapeutic strategies targeting NF-κB-related we developed computational approach predict drugs capable of inhibiting NF-κB signaling pathways. this study, utilized dataset comprising 1,149 inhibitors 1,332 non-inhibitors retrieved from PubChem. Chemical descriptors were computed using the PaDEL software, relevant features selected advanced feature selection techniques. Initially, machine learning models constructed 2D descriptors, 3D molecular fingerprints, achieving maximum AUC values 0.66, 0.56, respectively. To improve selection, applied univariate analysis SVC-L1 regularization identify that can effectively differentiate non-inhibitors. Using these features, models, our support vector classifier achieved an 0.75 on validation dataset. All trained five-fold cross-validation rigorously evaluated independent dataset, which was not used during training or hyperparameter optimization. Finally, best-performing model employed screen FDA-approved for potential inhibitors. Notably, most predicted corresponded previously identified as experimental studies, underscoring model’s predictive reliability. Our best- performing have been integrated into standalone software web server, NfκBin, enable scientific community perform high-throughput screening Nf-κB chemical libraries. ( https://webs.iiitd.edu.in/raghava/nfkbin/ ). Highlights 1) Inhibitors be manage disease like 2) A novel tool NfκBin library. 3) Cutting-edge techniques descriptors. 4) Models validated robust 5) Integrated server.

Language: Английский

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Ultra-small curcumin-ruthenium coordination polymer nanodots prevent renal ischemia-reperfusion injury and the progression to chronic kidney disease

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 14, 2025

Renal ischemia-reperfusion (IR) induces tissue hypoxia, resulting in disrupted energy metabolism and heightened oxidative stress. These factors contribute to tubular cell damage, which is a leading cause of acute kidney injury (AKI) can progress chronic disease (CKD). The excessive generation reactive oxygen species (ROS) plays crucial role the pathogenesis AKI. This study presents synthesis curcumin ultra-small coordination polymer (Ru/Cur) nanodots their application scavenging ROS renal tissues. By adding ruthenium ions methanol solution containing natural product curcumin, Ru/Cur were successfully synthesized. To enhance dispersibility these nanoparticles water, polyvinylpyrrolidone (PVP) was used as growth aid, highly stable with sizes smaller than 10 nm. results indicated that effectively eliminated various demonstrated significant therapeutic effects biocompatibility IR-AKI mice, reducing markers function alleviating stress, decreasing inflammatory infiltration. inhibited fibrosis by suppressing epithelial-mesenchymal transition secretion transforming factor-β1 model In summary, our findings confirm mitigate pathological conditions associated both AKI its progression CKD IR-induced injury.

Language: Английский

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Protective effect of Panax ginseng extract on cisplatin-induced AKI via downregulating cell death associated genes

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 25, 2025

This study is designed to assess the effect of root extract P. ginseng on kidney tissue injury attributed cisplatin and its molecular mechanism involved in this process AKI rat model. Twenty-four male Wistar rats were randomly allocated into 4 experimental groups including: control group, 100 mg/kg 200 group. The duration investigation was 7 days, all except group received a single dose 10 4th day. Our findings exhibited significant reduction blood concentration creatinine compared In severe renal histopathological alterations observed groups, significantly less damage than Ginseng showed minimal as 100. expression p21, p27, p53, TIMP2, IGFBP7, NF-κB decreased displayed amelioration cisplatin-induced dose-dependent decrease gene cell death-inducing genes by administration extract.

Language: Английский

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Curcumin and Its Derivatives in Hepatology: Therapeutic Potential and Advances in Nanoparticle Formulations

Cancers, Journal Year: 2025, Volume and Issue: 17(3), P. 484 - 484

Published: Feb. 1, 2025

Curcumin, a plant-derived polyphenol, shows promise in hepatology for treating both malignant and non-malignant liver diseases subset of extrahepatic cancers. Curcumin has hepatoprotective, anti-inflammatory, antifibrotic, antiproliferative properties, as is evident preclinical clinical studies. This highlights its potential an adjunct to established cancer therapies, especially the context hepatocellular carcinoma secondary malignancies. also demonstrates metabolic dysfunction-associated steatotic disease (MASLD), owing antifibrotic lipid-lowering effects. However, use limited, relating poor bioavailability rapid metabolism. Nanotechnology, including liposomal polymeric carriers, alongside synthetic curcumin derivatives, offers strategies enhance pharmacokinetic properties. We propose revisit nanoparticle preparations chronic summarize current evidence this review article.

Language: Английский

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Interruption of mitochondrial symbiosis is associated with the development of osteoporosis

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 3, 2025

Mitochondria maintain bacterial traits because of their endosymbiotic origins, yet the host cell recognizes them as non-threatening since organelles are compartmentalized. Nevertheless, controlled release mitochondrial components into cytoplasm can initiate death, activate innate immunity, and provoke inflammation. This selective interruption endosymbiosis early 2 billion years ago allowed mitochondria to become intracellular signaling hubs. Recent studies have found that symbiosis may be closely related occurrence various diseases, especially osteoporosis (OP). OP is a systemic bone disease characterized by reduced mass, impaired microstructure, elevated fragility, susceptibility fracture. The intra-mitochondrial affects energy metabolism cells, leads imbalance formation absorption, promotes osteoporosis. In this paper, we reviewed mechanism intersymbiosis in OP, discussed relationship between marrow mesenchymal stem osteoblasts osteoclasts, well inheritance adaptation evolutionary process, prospected future research direction provide new ideas for clinical treatment.

Language: Английский

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A cerium single-atom catalyst enables targeted catalytic therapy for acute kidney injury via neutrophil hitchhiking

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 380, P. 404 - 416

Published: Feb. 10, 2025

Language: Английский

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Nrf2 Signaling Pathway Studies in Drosophila melanogaster : Parallel Roles in Human Health and Insect Environmental Responses

Xenobiotica, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 30

Published: Feb. 11, 2025

The Nrf2 signaling pathway is crucial for cellular defense against oxidative stress and xenobiotic toxicity, highlighting its importance in both human health environmental responses.

Language: Английский

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DKS26 Alleviates Ischemia-Reperfusion Injury-Induced Acute Kidney Injury by Stabilizing Vitamin D Receptors to Inhibit the Inflammatory Pathway of NF-κB P65

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2985 - 2985

Published: March 25, 2025

Acute kidney injury (AKI) is a common critical clinical disease with high morbidity and mortality rates. Ischemia-reperfusion (IR) the main cause of AKI, there no effective treatment or prevention. Therefore, it to screen for therapeutic agents find targets. DKS26 derivative oleanolic acid (OA) optimized bioavailability while retaining anti-inflammatory, antioxidant, anti-apoptotic properties OA. This study aimed investigate effects on AKI its underlying molecular mechanisms. We established an model in vivo vitro observed that had ameliorative effect IR H/R-induced renal tubular epithelial cell reduced oxidative stress, inflammation, apoptosis. Meanwhile, Swiss TargetPrediction AutoDock Vina analysis revealed may interact vitamin D receptors (VDR) through hydrogen bonding, suggesting exert VDR. In this study, we found enhanced stability VDR protein, promoted binding p-NF-κB P65Ser311, entry P65Ser311 into nucleus, inhibited transcription downstream inflammatory genes as well their own expression, thus exerting protective effect. summary, these findings suggest be promising preventive strategy provide theoretical experimental basis treatment.

Language: Английский

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