Whole exome sequencing identifies novel variants of PIK3CA and validation of hotspot mutation by droplet digital PCR in Breast cancer among Indian Population. DOI Creative Commons
Rahul Kumar, Rakesh Kumar, Harsh Goel

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 19, 2023

Abstract Background - Breast cancer (BC) is the most common malignancy with very high incidence and relatively mortality in women. The PIK3CA gene plays a pivotal role pathogenicity of breast cancer. Despite these, mutational status all exons except 9 20 still remains unknown. Methods This study uses whole exome sequencing (WES) based approach to identify somatic mutations Indian BC cohorts. resultant hotspot were validated by droplet digital PCR (ddPCR). Further, molecular dynamics (MD) simulation was applied elucidate conformational functional effects position on protein. Results In our cohort, showed 44.4% mutation rate among top mutated genes. confined Exons 5, 9, 11, 18, 20, whereas maximum number lies within 20. A total variants found study, which 2 novel observed (p.H554L) 11 (p.S629P). However, H1047R at exon (20%). tumor tissues, there considerable difference between copy wild-type mutant detected ddPCR. Significant structural changes during MD induced due point H1047R/L position. Conclusions current provides comprehensive view as well reported single nucleotide (SNVs) associated cases. could serve prognostic value terms selecting targeted therapy BC.

Language: Английский

Synergistic enhancement: Exploring the potential of piperine in cancer therapeutics through chemosensitization and combination therapies DOI

Mukesh Kumar Manickasamy,

Aviral Kumar, Bandari BharathwajChetty

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: 354, P. 122943 - 122943

Published: Aug. 6, 2024

Language: Английский

Citations

1
Recent advances in Scutellariae radix: A comprehensive review on ethnobotanical uses, processing, phytochemistry, pharmacological effects, quality control and influence factors of biosynthesis DOI Creative Commons
Wentao Ma, Tianyu Liu, Omachi Daniel Ogaji

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e36146 - e36146

Published: Aug. 1, 2024

Highlights•SR is an attractive herb with multiple traditional efficacy and excellent prospects.•The main components of SR are flavonoids which have various pharmacological effects.•The historical applications, processing, biosynthesis summarized.•The quality markers related to the biological activity yet be explord.AbstractBackgroundScutellariae radix (SR) dried root Scutellaria baicalensis Georgi. It has a long history ethnic medicinal use, traditionally recognized for its in clearing heat, drying dampness, eliminating fire, removing toxins, stopping bleeding tranquilizing fetus prevent miscarriage. Clinically, it used treat cold, fever, migraine, hand-foot-and-mouth diseases, liver cancer inflammatory diseases.PurposeThe review aims provide comprehensive reference on ethnobotanical uses, phytochemistry, effect, control influence factors deeper understanding SR.Results conclusionA total 210 isolated been reported literature, including their glycosides, phenylpropanoids, phenylethanoid phenolic acids, volatile components, polysaccharides others. The extract such as baicalin, baicalein, wogonin, wogonoside, scutellarin showed antioxidant, anti-inflammatory, anti-tumor, antiviral, hepatoprotective, neuroprotective effects. However, further studies required elucidate mechanisms action clinical applications. pharmacodynamic evaluation based should conducted. Although analytical methods established SR, there gaps research regarding efficacy-related development standards processed products. regulatory remain explored while environmental transcription studied. In conclusion, promising herbal medicine significant potential future development.Graphical abstract

Language: Английский

Citations

1
Antagonistic Activity of Oroxylin A against Fusarium graminearum and Its Inhibitory Effect on Zearalenone Production DOI Creative Commons
Luli Zhou, Guanyu Hou, Hanlin Zhou

et al.

Toxins, Journal Year: 2023, Volume and Issue: 15(9), P. 535 - 535

Published: Aug. 31, 2023

Fusarium graminearum produces zearalenone (ZEA), a mycotoxin that is widely found in food and feed products toxic to humans livestock. Piper sarmentosum extract (PSE) inhibits F. graminearum, Oroxylin A appears be major antifungal compound PSE. The aim of this study quantify the content PSE using UPLC-QTOF-MS/MS, investigate antagonistic activity against its inhibitory effect on ZEA production. results indicate both fungal growth production dose-dependent manner. treatment downregulated mRNA expression biosynthesis protein 1 (ZEB1) 2 (ZEB2). metabolomics analysis mycelia indicated level ribose 5-phosphate (R5P) deceased (p < 0.05) after (64-128 ng/mL). Moreover, as increased from 64 128 ng/mL, levels cis-aconitate fumarate 0.01) were upregulated successively. correlation further showed decreased R5P was positively correlated with ZEB1 ZEB2 expression, while negatively expression. These findings demonstrate potential natural agent control toxigenic fungi their mycotoxin.

Language: Английский

Citations

3
Whole exome sequencing identifies novel variants of PIK3CA and validation of hotspot mutation by droplet digital PCR in breast cancer among Indian population DOI Creative Commons
Rahul Kumar, Rakesh Kumar, Harsh Goel

et al.

Cancer Cell International, Journal Year: 2023, Volume and Issue: 23(1)

Published: Oct. 11, 2023

Abstract Background Breast cancer (BC) is the most common malignancy with very high incidence and relatively mortality in women. The PIK3CA gene plays a pivotal role pathogenicity of breast cancer. Despite this, mutational status all exons except 9 20 still remains unknown. Methods This study uses whole exome sequencing (WES) based approach to identify somatic mutations Indian BC cohorts. resultant hotspot were validated by droplet digital PCR (ddPCR). Further, molecular dynamics (MD) simulation was applied elucidate conformational functional effects position on protein. Results In our cohort, showed 44.4% mutation rate among top mutated genes. confined Exons 5, 9, 11, 18, 20, whereas maximum number lies within 20. A total variants found study, which 2 novel observed (p.H554L) 11 (p.S629P). However, H1047R at exon (20%). tumor tissues, there considerable difference between copy wild-type mutant detected ddPCR. Significant structural changes during MD simulation, induced due point H1047R/L position. Conclusions current provides comprehensive view as well reported single nucleotide (SNVs) associated cases. could serve prognostic value terms selecting targeted therapy BC.

Language: Английский

Citations

2
Crosstalk between Non-Coding RNAs and Wnt/β-Catenin Signaling in Head and Neck Cancer: Identification of Novel Biomarkers and Therapeutic Agents DOI Creative Commons
Anjana Sajeev, Bandari BharathwajChetty, Ravichandran Vishwa

et al.

Non-Coding RNA, Journal Year: 2023, Volume and Issue: 9(5), P. 63 - 63

Published: Oct. 17, 2023

Head and neck cancers (HNC) encompass a broad spectrum of neoplastic disorders characterized by significant morbidity mortality. While contemporary therapeutic interventions offer promise, challenges persist due to tumor recurrence metastasis. Central HNC pathogenesis is the aberration in numerous signaling cascades. Prominently, Wnt pathway has been critically implicated etiology HNC, as supported plethora research. Equally important, variations expression non-coding RNAs (ncRNAs) have identified modulate key cancer phenotypes such cellular proliferation, epithelial-mesenchymal transition, metastatic potential, recurrence, treatment resistance. This review aims provide an exhaustive insight into multifaceted influence ncRNAs on with specific emphasis their interactions Wnt/β-catenin (WBC) axis. We further delineate effect either exacerbating or attenuating progression via interference WBC signaling. An overview mechanisms underlying interplay between also presented. In addition, we described potential various enhancing efficacy chemotherapeutic radiotherapeutic modalities. summary, this assessment posits agents targeting management.

Language: Английский

Citations

2
Mitochondrial ROS, a trigger for mitochondrial dysfunction and inflammasome activation and a therapeutic target in liver diseases DOI Creative Commons

Hala Saeed Jaara,

Sandra Torres

Published: Dec. 10, 2024

Mitochondria are essential organelles responsible for intracellular energy production and play crucial roles in cellular metabolism, inflammation, apoptosis. Reactive oxygen species (ROS) primarily produced the mitochondria endoplasmic reticulum of hepatocytes due to activity cytochrome P450 enzymes. Under ideal conditions, cells have specific molecular mechanisms that manage oxidative stress levels, thus ensuring a balance between oxidants antioxidants. The interplay ROS-induced mitochondrial dysfunction activation NLRP3 (nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3) inflammasome context liver diseases has been extensively studied. However, exact by which promote contribute onset disease remain unclear. This review aims elucidate recently discovered regulation disorders, including alcohol-related (ALD), metabolic-associated steatotic (MASLD), hepatocellular carcinoma (HCC). Finally, it summarizes various natural pharmaceutical agents can mitigate damage modulating through pathways. work serves as an important resource identifying new therapeutic approaches provides further support advancing understanding diseases.

Language: Английский

Citations

0
Whole exome sequencing identifies novel variants of PIK3CA and validation of hotspot mutation by droplet digital PCR in Breast cancer among Indian Population. DOI Creative Commons
Rahul Kumar, Rakesh Kumar, Harsh Goel

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 19, 2023

Abstract Background - Breast cancer (BC) is the most common malignancy with very high incidence and relatively mortality in women. The PIK3CA gene plays a pivotal role pathogenicity of breast cancer. Despite these, mutational status all exons except 9 20 still remains unknown. Methods This study uses whole exome sequencing (WES) based approach to identify somatic mutations Indian BC cohorts. resultant hotspot were validated by droplet digital PCR (ddPCR). Further, molecular dynamics (MD) simulation was applied elucidate conformational functional effects position on protein. Results In our cohort, showed 44.4% mutation rate among top mutated genes. confined Exons 5, 9, 11, 18, 20, whereas maximum number lies within 20. A total variants found study, which 2 novel observed (p.H554L) 11 (p.S629P). However, H1047R at exon (20%). tumor tissues, there considerable difference between copy wild-type mutant detected ddPCR. Significant structural changes during MD induced due point H1047R/L position. Conclusions current provides comprehensive view as well reported single nucleotide (SNVs) associated cases. could serve prognostic value terms selecting targeted therapy BC.

Language: Английский

Citations

0