CMSP exerts anti-tumor effects on small cell lung cancer cells by inducing mitochondrial dysfunction and ferroptosis

Open Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

This study aims to investigate the role and mechanism of p-hydroxyl cinnamaldehyde (CMSP) in triggering ferroptosis small cell lung cancer (SCLC) cells. The impact CMSP on H1688 SW1271 cells was assessed through experiments biological information analysis. Moreover, expression heme oxygenase 1 (HMOX1) SCLC tissue examined. Following treatment, a concentration-dependent increase death observed, differentially expressed genes were found be associated with ferroptosis. notably facilitated events, such as elevated levels reactive oxygen species (ROS), Fe2+, malondialdehyde (MDA), transferrin receptor (TFR1), divalent metal transporter (DMT1), decreased glutathione (GSH), solute carrier family 7 member 11 (SLC7A11), peroxidase 4 (GPX4). Furthermore, promoted mitochondrial dysfunction, manifested reduced volume, increased membrane density, ROS, potential. Consistently, mitochondrial-targeted antioxidant Mito-TEMPO reversed CMSP-induced Expression HMOX1 gene markedly under while lower observed compared adjacent tissue. triggers dysfunction via activation, leading cells, underscoring its potential therapeutic agent for SCLC.

Language: Английский

---

Sculponeatin A induces mitochondrial dysfunction in non‐small cell lung cancer through WWP2‐mediated degradation of mitochondrial STAT3

British Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Abstract Background and Purpose The phosphorylation of signal transducer activator transcription 3 (STAT3) monomer at S727 promotes its mitochondrial localisation regulates function, thus exerting a protective effect on tumour cells. However, no inhibitor drugs targeting STAT3 (mitoSTAT3) or S727‐STAT3 have been identified. Here, we report novel diterpenoid extracted from Isodon sculponeatus , sculponeatin A (sptA), induces dysfunction in non‐small cell lung cancer (NSCLC) by mitoSTAT3 degradation. Experimental Approach xCELLigence real‐time analysis assay high‐content were performed to measure cytotoxicity. Mitochondrial function was assessed transmission electron microscopy, permeability transition pore opening Seahorse cellular flux assays. effects sptA the upstream signalling pathway measured Western blot, gene alterations other approaches. Immunofluorescence live imaging visualise expression position mitoSTAT3. Nude mice zebrafish modelled with subcutaneous xenografts. Pharmacokinetics examined rats. Drug toxicity evaluated zebrafish. Key Results inhibited respiration NSCLC induced promoting degradation promoted WW domain containing E3 ubiquitin protein ligase 2 (WWP2)‐mediated ubiquitination through direct binding. growth vivo. Evaluation drug showed that overdose may cause heart damage. Conclusions Implications These findings suggest pharmacological provide therapeutic benefits against NSCLC.

Language: Английский

---

The potential of natural herbal plants in the treatment and prevention of non-small cell lung cancer: An encounter between ferroptosis and mitophagy

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119555 - 119555

Published: Feb. 1, 2025

Language: Английский

---

Eudragit S 100 Assisted Molecular Solid Dispersion of Andrographolide Tendered Augmented Drug Delivery and Apoptosis in Human Colon Cancer, HT-29 Cells

AAPS PharmSciTech, Journal Year: 2025, Volume and Issue: 26(3)

Published: March 11, 2025

Language: Английский

---

Insights into emerging mechanisms of ferroptosis: new regulators for cancer therapeutics

Cell Biology and Toxicology, Journal Year: 2025, Volume and Issue: 41(1)

Published: March 25, 2025

Ferroptosis is an iron-dependent form of regulated cell death characterized by the accumulation lipid peroxides, which has been implicated in pathogenesis various diseases, and therapeutic agents targeting ferroptosis are emerging as promising tools for cancer treatment. Current research reveals that ferroptosis-targeted therapies can effectively inhibit tumor progression or delay development. Notably, natural product-derived compounds-such artemisinin, baicalin, puerarin, quercetin, kaempferol, apigenin-have demonstrated ability to modulate ferroptosis, offering potential anti-cancer benefits. Mechanistically, exhibits negative glutathione peroxidase 4 (GPX4) regulation demonstrates a positive correlation with plasma membrane polyunsaturated fatty acid (PUFA) abundance. Moreover, labile iron pool (LIP) serves redox engine ferroptosis. This review systematically analyzes hallmarks, signaling pathways, molecular mechanisms focus on how small molecules regulate this process. It further evaluates their inducers inhibitors anti-tumor therapy, providing foundation future clinical translation.

Language: Английский

---

Ferroptosis: Therapeutic Potential and Strategies in Non-Small Cell Lung Cancer

Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 545 - 545

Published: May 14, 2025

Non-small cell lung cancer (NSCLC) is the most common subtype of and a leading cause cancer-related morbidity mortality worldwide. Despite advancements in therapeutic strategies, prognosis for NSCLC patients remains unfavorable. The effective treatment challenging due to its aggressive metastatic invasive properties. Therefore, there an urgent need explore novel strategies. In recent years, different from apoptosis necrosis, ferroptosis has garnered increasing attention since initial identification 2012. It increasingly recognized as key factor development progression various cancers. this review, we summarize distinctive morphological biochemical characteristics regulatory mechanisms. Furthermore, discuss genetic regulation NSCLC, highlighting biomarkers that may serve potential targets. We also evaluate emerging strategies targeting ferroptosis, including gene therapy, natural compounds, chemical agents, combination therapies, nanoparticle-based approaches. Based on current evidence, limitations future prospects ferroptosis-based therapies are discussed. This review aims provide insights into implications treatments.

Language: Английский

---

Knockdown of KBTBD7 attenuates septic lung injury by inhibiting ferroptosis and improving mitochondrial dysfunction

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 133, P. 112129 - 112129

Published: April 23, 2024

Language: Английский

---

Ferroptosis: emerging roles in lung cancer and potential implications in biological compounds

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: May 9, 2024

Lung cancer has high metastasis and drug resistance. The prognosis of lung patients is poor the patients’ survival chances are easily neglected. Ferroptosis a programmed cell death proposed in 2012, which differs from apoptosis, necrosis autophagy. novel type regulated driven by iron-dependent lipid peroxidation subsequent plasma membrane ruptures. It broad prospects field tumor disease treatment. At present, multiple studies have shown that biological compounds can induce ferroptosis cells, exhibits significant anti-cancer effects, they advantages safety, minimal side less possibility to In this review, we summarize used for treatment focusing on its mechanism. addition, systematically review current research status combining nanotechnology with treatment, shed new light targeting pathways applying compounds-based therapies.

Language: Английский

---

A novel selenium nanocomposite modified by AANL inhibits tumor growth by upregulating CLK2 in lung cancer

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 148, P. 107459 - 107459

Published: May 15, 2024

Language: Английский

---

The therapeutic potential of andrographolide in cancer treatment

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 180, P. 117438 - 117438

Published: Sept. 19, 2024

Language: Английский

---