Osthole ameliorates wear particle-induced osteogenic impairment by mitigating endoplasmic reticulum stress via PERK signaling cascade DOI Creative Commons
Xin Yu, Juan Jiang, Li Cheng

et al.

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: Dec. 20, 2024

Abstract Background Periprosthetic osteolysis and subsequent aseptic loosening are the leading causes of failure following total joint arthroplasty. Osteogenic impairment induced by wear particles is regarded as a crucial contributing factor in development osteolysis, with endoplasmic reticulum (ER) stress identified key underlying mechanism. Therefore, identifying potential therapeutic targets agents that can regulate ER adaption osteoblasts necessary for arresting loosening. Osthole (OST), natural coumarin derivative, has demonstrated promising osteogenic properties ability to modulate various diseases. However, impact OST on stress-mediated caused remains unclear. Methods TiAl 6 V 4 (TiPs) were sourced from prosthesis patients who underwent revision hip arthroplasty due A mouse calvarial model was utilized explore effects TiPs-induced vivo. Primary employed investigate osteoblast apoptosis inhibition TiPs vitro. The mechanisms OST-modulated alleviation elucidated through Molecular docking, immunochemistry, PCR, Western blot analysis. Results In this study, we found treatment effectively mitigated (TiPs)-induced enhancing osteogenesis model. Furthermore, observed could attenuate reduction exposed vitro Mechanistically, exerts bone-sparing stressed upon exposure specifically suppressing stress-dependent PERK signaling cascade. Conclusion ameliorates particle-induced mitigating via These findings suggest may serve agent combating impairment, offering novel alternative strategy managing

Language: Английский

Deep eutectic solution elution assisted ligand affinity assay: a useful tool for the active coumarins screening from Fructus cnidii DOI

Jianing Su,

Bing Li, Kun Yao

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1336, P. 343481 - 343481

Published: Nov. 26, 2024

Language: Английский

Citations

0
Osthole ameliorates wear particle-induced osteogenic impairment by mitigating endoplasmic reticulum stress via PERK signaling cascade DOI Creative Commons
Xin Yu, Juan Jiang, Li Cheng

et al.

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: Dec. 20, 2024

Abstract Background Periprosthetic osteolysis and subsequent aseptic loosening are the leading causes of failure following total joint arthroplasty. Osteogenic impairment induced by wear particles is regarded as a crucial contributing factor in development osteolysis, with endoplasmic reticulum (ER) stress identified key underlying mechanism. Therefore, identifying potential therapeutic targets agents that can regulate ER adaption osteoblasts necessary for arresting loosening. Osthole (OST), natural coumarin derivative, has demonstrated promising osteogenic properties ability to modulate various diseases. However, impact OST on stress-mediated caused remains unclear. Methods TiAl 6 V 4 (TiPs) were sourced from prosthesis patients who underwent revision hip arthroplasty due A mouse calvarial model was utilized explore effects TiPs-induced vivo. Primary employed investigate osteoblast apoptosis inhibition TiPs vitro. The mechanisms OST-modulated alleviation elucidated through Molecular docking, immunochemistry, PCR, Western blot analysis. Results In this study, we found treatment effectively mitigated (TiPs)-induced enhancing osteogenesis model. Furthermore, observed could attenuate reduction exposed vitro Mechanistically, exerts bone-sparing stressed upon exposure specifically suppressing stress-dependent PERK signaling cascade. Conclusion ameliorates particle-induced mitigating via These findings suggest may serve agent combating impairment, offering novel alternative strategy managing

Language: Английский

Citations

0