American journal of biochemistry & biotechnology/American journal of biochemistry and biotechnology,
Journal Year:
2023,
Volume and Issue:
19(4), P. 336 - 346
Published: April 1, 2023
Trilobatin,
a
natural
dihydrochalcone
compound,
possesses
various
biological
activities.
However,
few
investigations
have
been
carried
out
on
the
mechanisms
of
trilobatin
against
oxidative
stress.
To
comprehend
effects
and
mechanism
stress
after
hepatic
injury,
H2O2-induced
HepG2
cells
were
used
in
this
research.
The
results
indicated
that
enhanced
viability
improved
apoptosis
morphology
induced
by
H2O2.
decrease
Mitochondrial
Membrane
Potential
(MMP)
increase
antioxidant-related
enzymes
involving
Superoxide
Dismutase
(SOD),
Glutathione
Peroxidase
(GSH-Px),
Catalase
(CAT),
as
well
reduced
levels
alanine
Aminotransferase
(ALT)
aspartate
(AST),
could
be
responsible
for
protective
effect
cells.
Furthermore,
Nuclear
erythroid-2
Related
Factor
2
(Nrf2)/Kelch-like
ECH
Associated
Protein-1
(Keap-1)
pathway
was
activated
These
has
strong
capacity
inhibiting
via
Nrf2/Keap1
signaling
pathway.
This
research
implies
might
useful
antioxidant
to
prevent
liver
diseases
caused
Plants,
Journal Year:
2024,
Volume and Issue:
13(23), P. 3389 - 3389
Published: Dec. 3, 2024
Antioxidant
activity
and
flavonoid
content
are
important
characteristics
of
colored
rice
grains.
Previously,
we
obtained
a
preliminary
understanding
the
metabolic
markers
antioxidant
activity,
namely,
phlorizin
trilobatin,
in
different
varieties
purple
grains
at
growth
stages,
but
mechanisms
associated
with
these
have
not
yet
been
confirmed.
In
this
study,
was
selected
as
experimental
material,
clover
extract
applied
during
grain-filling
stage
to
explore
impact
on
total
capacity
biosynthesis
The
results
indicated
that
content,
phenolic
oligomeric
proanthocyanidin
treated
an
exogenous
application
trilobatin
(T30)
were
significantly
greater
than
those
control
(CK).
flavonoids
T30
CK
groups
accounted
for
29.81%
flavonoids.
phenylpropanoid
pathways
constructed
basis
differentially
abundant
metabolites
between
groups.
Additionally,
1-O-sinapoyl-beta-D-glucose,
coniferaldehyde,
6″-acetylapiin,
kaempferol-3-O-rutinoside
determined
be
essential
trilobatin-mediated
rice.
correlation
network
diagram
biochemical
indexes
revealed
metabolites.
This
study
provides
scientific
improving
nutritional
quality
pathway.
Journal of Traditional Chinese Medical Sciences,
Journal Year:
2023,
Volume and Issue:
11(1), P. 55 - 66
Published: Dec. 6, 2023
To
explore
and
validate
the
potential
targets
of
Paeoniae
Radix
Alba
(P.
Radix,
Bai
Shao)
in
protecting
against
chemical
liver
injury
through
network
pharmacology,
molecular
docking
technology,
vitro
cell
experiments.
Network
pharmacology
was
used
to
identify
common
P.
injury.
Molecular
fit
components,
which
were
subsequently
verified
vitro.
A
model
hepatic
fibrosis
established
by
activating
stellate
(HSC)-LX2
cells
with
10
ng/mL
transforming
growth
factor-β1.
The
treated
various
concentrations
total
glucosides
paeony
(TGP),
active
substance
then
their
viability
assessed
using
Cell
Counting
Kit-8
assay.
expression
levels
heat
shock
protein
(HSP)-90α,
prostaglandin
endoperoxide
synthase
2
(PTGS2),
interleukin-6
(IL-6),
interleukin-1β
(IL-1β),
tumor
necrosis
factor
α
(TNF-α)
measured
enzyme-linked
immunosorbent
assay,
whereas
collagen
type
I
(COL-I),
Ⅲ
(COL-III),
peroxisome
proliferator-activated
receptor
(PPAR)-γ,
caspase
3
(CASP3)
detected
western
blot.
Analysis
revealed
13
key
compounds
for
preventing
IL-6,
AKT
serine/threonine
kinase
1,
jun
proto-oncogene,
90
alpha
family
class
member
1
(HSP90AA1),
proliferator
activated
gamma
(PPARG),
PTGS2,
CASP3.
Gene
Ontology
(GO)
enrichment
analysis
indicated
involvement
response
drugs,
membrane
rafts,
peptide
binding.
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
that
main
pathways
involved
lipid
atherosclerosis
carcinogenesis-receptor
activation.
Paeoniflorin
albiflorin
exhibited
strong
affinity
HSP90AA1,
PPARG,
Different
TGP
can
inhibit
COL-Ⅰ,
COL-Ⅲ,
TNF-α,
IL-1β,
HSP-90α,
PTGS2
while
increasing
PPAR-γ
CASP3
HSC-LX2
cells.
protective
effect
involves
multiple
targets,
pathways.
primarily
functions
regulating
such
as
CASP3,
others.
TGP,
compound
protects
reducing
inflammatory
response,
apoptotic
proteins,
promoting
apoptosis
HSCs.
In
future,
mechanism
action
be
studied
from
two
perspectives:
apoptosis.
American journal of biochemistry & biotechnology/American journal of biochemistry and biotechnology,
Journal Year:
2023,
Volume and Issue:
19(4), P. 336 - 346
Published: April 1, 2023
Trilobatin,
a
natural
dihydrochalcone
compound,
possesses
various
biological
activities.
However,
few
investigations
have
been
carried
out
on
the
mechanisms
of
trilobatin
against
oxidative
stress.
To
comprehend
effects
and
mechanism
stress
after
hepatic
injury,
H2O2-induced
HepG2
cells
were
used
in
this
research.
The
results
indicated
that
enhanced
viability
improved
apoptosis
morphology
induced
by
H2O2.
decrease
Mitochondrial
Membrane
Potential
(MMP)
increase
antioxidant-related
enzymes
involving
Superoxide
Dismutase
(SOD),
Glutathione
Peroxidase
(GSH-Px),
Catalase
(CAT),
as
well
reduced
levels
alanine
Aminotransferase
(ALT)
aspartate
(AST),
could
be
responsible
for
protective
effect
cells.
Furthermore,
Nuclear
erythroid-2
Related
Factor
2
(Nrf2)/Kelch-like
ECH
Associated
Protein-1
(Keap-1)
pathway
was
activated
These
has
strong
capacity
inhibiting
via
Nrf2/Keap1
signaling
pathway.
This
research
implies
might
useful
antioxidant
to
prevent
liver
diseases
caused
