A new target for treating intervertebral disk degeneration: gut microbes DOI Creative Commons
Kaizhong Wang, Xiangyan Liu,

Huagui Huang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 29, 2024

Intervertebral disk degeneration (IDD) is a common clinical spinal disease and one of the main causes low back pain (LBP). Generally speaking, IDD considered natural degenerative process with age. However, deepening research, people have discovered that not only related to age, but also has many factors can induce accelerate its progression. In addition, pathogenesis remains unclear, resulting in limited traditional treatment methods cannot effectively prevent treat IDD. Conservative may lead patients’ dependence on drugs, relief effect obvious. Similarly, surgical highly invasive, longer recovery time higher recurrence rate. With exploration, intestinal microorganisms are an important symbiotic microbial community human body closely occurrence development various diseases. Changes their metabolites affect body’s inflammatory response, immune regulation, metabolic processes, thereby affecting health intervertebral disk. this context, gut microbiota received considerable attention as potential target for delaying or treating This article first introduces impact microbes distal organs, then focuses three mechanisms by which influence Finally, we summarized interfering metabolites. Further understanding between will help formulate reasonable strategies achieve ideal therapeutic effects.

Language: Английский

Dachaihu decoction ameliorates abnormal behavior by regulating gut microbiota in rats with propionic acid-induced autism DOI Creative Commons
Yangyang Zhang, Hang Li, Bolin Li

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 13, 2025

Background Autism spectrum disorder (ASD) is an early-onset neurodevelopmental disorder, usually accompanied by gut microbiota dysregulation. Gut homeostasis considered effective for ASD. Reportedly, Dachaihu decoction (DCHD) can efficiently regulate and inflammation. However, the mechanisms underlying effects of DCHD in treatment ASD remain unclear. Objective This study investigated potential treating Methods In animal experiment, propionic acid was administered to construct rat model. The rats were treated with DCHD, efficacy assessed using behavioral detections, such as open field test, elevated plus maze novel object recognition test. Additionally, levels IL-6, TNF- α , IL-10, T-SOD, MDA, GSH CAT determined kits, histological staining used evaluate brain morphology. Moreover, tight junction proteins (ZO-1 occludin) expression evaluated RT-qPCR, whereas Iba1 level immunofluorescence staining. 16S rRNA sequencing metabolomic analysis feces revealed targets against a small human trail, clinical scales ADOS-2 Behavior Checklist (ABC) autism severity. Gastrointestinal problems function based on food intolerance event-related potential, respectively. Results significantly improved autism-like behaviors increased antioxidant enzyme activity, decreased inflammation enhanced intestinal barrier experiment. Furthermore, altered profile, probiotics Adlercreutzia Parvibacter Turicibacter Christensenellaceae. Further, beneficial metabolite indole-3-acetate cognitive impairment-related metabolites asymmetric dimethylarginine homogentisic acid. Meanwhile, trial that alleviated core symptoms ASD, ABC scale scores. also specific egg white/yolk milk IgG antibodies shortened MMN P3b latencies. Conclusion demonstrated may alleviate via inhibiting oxidative stress, reducing inflammation, modulating rats. Combined trial, be promising drug provides scientific rationale mental disorders related dysbiosis.

Language: Английский

Citations

0
Crosslinking Stabilization Strategy: A Novel Approach to Cartilage-like Repair of Annulus Fibrosus (AF) Defects DOI Creative Commons
Zihan Wang, Lei Wang,

Shaoshuo Li

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101625 - 101625

Published: March 5, 2025

Lumbar disc degeneration due to annulus fibrosus (AF) defects poses a significant challenge in clinical treatment Current treatments exhibit limited repair efficacy and high recurrence rate. To address this, we devised novel approach of crosslinking stabilization strategy. We integrated fibrinogen, thrombin, genipin, human bone marrow-derived mesenchymal stem cells (hBMSCs) hydrogel (FTGB) with acellular scaffold fascia (FTGB@S@F) remediate AF defects. FTIR analysis confirmed stable chemical within the FTGB hydrogel. demonstrated superior biocompatibility compared FB hydrogel, significantly higher cell viability (97.60 ± 2.02 % vs 81.43 4.50 %, P < 0.01) enhanced proliferation migration, as shown DAPI, Edu phalloidin staining. Atomic force microscopy (AFM) revealed that FTGB@S has dense reticular structure, enhancing material performance elastic modulus than FB@S. MTS testing showed FTGB@S@F outperformed other groups resisting cyclic axial load (25.53 1.17 MPa) maintaining height (0.57 0.12 mm), compression resistance minimal deformation. It also exhibited lowest rupture ROM (1.45 0.17 mm) close Intact control, demonstrating its potential restore mechanical function. MRI imaging group preserved an intact structure signal intensity, larger NP area (223.64 73.32 mm2 137.30 75.31 mm2, 0.05), (102.5 88.50 12.86 0.05). Histology reduced Un-repair FB@S@F groups. Transcriptomic identified upregulation PIGR downregulation COL4A3, linked PI3K-Akt pathway. Immunohistochemical qPCR analyses expression COL1, Aggrecan, RhoA, indicating effective regeneration.

Language: Английский

Citations

0
Exploring the role of gut microbiota in intervertebral disc degeneration: insights from bidirectional Mendelian randomization analysis DOI Creative Commons

Jiheng Xiao,

Xianglong Zhou, Tianyi Xia

et al.

European Spine Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Although previous studies have indicated a potential association between the gut microbiota and intervertebral disc degeneration (IVDD), precise nature of this relationship remains unclear. The objective study is to further explore causal IVDD using bidirectional Mendelian randomization approach, with aim identifying microbial characteristics associated IVDD. Using data from genome-wide (GWAS) involving 412 species 227,388 controls 29,508 cases Inverse variance weighted (IVW) was used as primary (MR) analysis, complemented by median, MR-Egger regression, mode simple methods. Extensive sensitivity analyses were performed confirm robustness results assess heterogeneity horizontal pleiotropy. This revealed positive genetic predisposition 6 types through IVW method, indicating that increased levels these may lead higher risk Conversely, found negative effects on IVDD, suggesting protective effect against Reverse MR analysis such possibilities 1 5 relationships microbiota. Cochran's Q test, MR-PRESSO all yielded p-values greater than 0.05, there no significant or pleiotropy in We approach identify various Our findings lay foundation for exploration pathogenesis treatment strategies provide new research biomarkers IVDD-related metabolic

Language: Английский

Citations

0
Unveiling the Gut-Disc Axis: How Microbiome Dysbiosis Accelerates Intervertebral Disc Degeneration DOI Creative Commons

Yu Sun,

Zhaoyong Li, Jiahao Duan

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 8271 - 8280

Published: Nov. 1, 2024

Abstract: The gut microbiome (GM), often referred to as the second genome of human body, plays a crucial role in various metabolic processes and mediates development numerous diseases. Intervertebral disc degeneration (IDD) is an age-related degenerative spinal disease characterized by loss height, hydration, integrity, leading pain reduced mobility. Although pathogenesis IDD not fully understood, recent studies suggest that dysbiosis may accelerate progression through multiple mechanisms. This article begins discussing potential relationship between GM diseases, followed comprehensive review regulatory mechanisms skeletal diseases within gut-disc axis framework. Furthermore, it explores three pathways which mediate IDD: immunomodulation, bacterial translocation colonization, decomposition absorption intestinal metabolites. These can disrupt cell homeostasis promote changes. Finally, this paper summarizes for first time therapeutic approaches delaying targeting axis, providing new insights into regenerative repair strategies IDD. Keywords: intervertebral degeneration, microbiome, inflammation, immunity, metabolism

Language: Английский

Citations

2
Integrative microbiomics, proteomics and lipidomics studies unraveled the preventive mechanism of Shouhui Tongbian Capsules on cerebral ischemic stroke injury DOI

Fangjiao Wei,

Jidong Zhou, Lihong Pan

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 118874 - 118874

Published: Oct. 1, 2024

Language: Английский

Citations

1
The role of micronutrients and serum metabolites in intervertebral disk degeneration: insights from a Mendelian randomization study and mediation analysis DOI Creative Commons

Nizhou Jiang,

Quanxiang Wang, Jian Jiang

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 21, 2024

Background Intervertebral disk degeneration (IVDD) is a complex degenerative skeletal condition, potentially influenced by micronutrients and serum metabolites in its etiology. However, the exact causal relationship between these factors IVDD remains ambiguous. Methods The research employed Two-Sample Mendelian Randomization (2SMR) analysis to thoroughly evaluate 15 (consisting of 7 minerals 8 vitamins) as exposure variables, 1,091 blood metabolites, 309 metabolite ratios intermediary factors, outcome. Additionally, reverse MR mediation were carried out validate reliability results explore underlying mechanism which influence risk regulating metabolites. Results Among examined, vitamin B12 exhibited noteworthy negative correlation with incidence (OR: 0.752, 95% [CI]: 0.573–0.987, p = 0.040), indicating potential reduction increased consumption. Of analyzed, 52 displayed significant associations IVDD, primarily linked amino acid, fatty nucleotide, sphingolipid metabolic pathways. Mediation identified 4-acetaminophen sulfate mediator protective effect against IVDD. Conclusion This study has shown that may reduce are associated Furthermore, it proposes could serve exerts inhibitory effects on

Language: Английский

Citations

0
Crosslinking Stabilization Strategy: A Novel Approach to Cartilage-Like Repair of Annulus Fibrosus (Af) Defects DOI
Zihan Wang, Lei Wang,

Shaoshuo Li

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
A new target for treating intervertebral disk degeneration: gut microbes DOI Creative Commons
Kaizhong Wang, Xiangyan Liu,

Huagui Huang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 29, 2024

Intervertebral disk degeneration (IDD) is a common clinical spinal disease and one of the main causes low back pain (LBP). Generally speaking, IDD considered natural degenerative process with age. However, deepening research, people have discovered that not only related to age, but also has many factors can induce accelerate its progression. In addition, pathogenesis remains unclear, resulting in limited traditional treatment methods cannot effectively prevent treat IDD. Conservative may lead patients’ dependence on drugs, relief effect obvious. Similarly, surgical highly invasive, longer recovery time higher recurrence rate. With exploration, intestinal microorganisms are an important symbiotic microbial community human body closely occurrence development various diseases. Changes their metabolites affect body’s inflammatory response, immune regulation, metabolic processes, thereby affecting health intervertebral disk. this context, gut microbiota received considerable attention as potential target for delaying or treating This article first introduces impact microbes distal organs, then focuses three mechanisms by which influence Finally, we summarized interfering metabolites. Further understanding between will help formulate reasonable strategies achieve ideal therapeutic effects.

Language: Английский

Citations

0