Biomarkers: New Advances in Diabetic Nephropathy

Natural Product Communications, Journal Year: 2025, Volume and Issue: 20(2)

Published: Feb. 1, 2025

Diabetic nephropathy (DN) is the leading cause of uremia and clinical mortality, characterized by progressive deterioration kidney structure function due to prolonged exposure hyperglycemia. This condition often necessitates renal replacement therapy for patients with end-stage-renal-disease (ESRD). Consequently, early detection, diagnosis, treatment DN are crucial mitigate disease progression, enhance patient outcomes, maintain a good quality life. Exploring relevant biomarkers diagnosis holds significant importance. In recent years, numerous researchers have identified various novel associated diabetic nephropathy, which critical predicting both onset progression disease. article aims provide comprehensive overview related DN.

Language: Английский

Microbiome and metabolome explain the high-fat diet-induced diabetes development and diabetes resistance in Guizhou mini-pigs

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 9, 2025

Type 2 diabetes mellitus (T2DM) is an obesity-related disease claiming substantial global mortality annually. Current animal models of T2DM remain limited, with low success rates in establishing porcine high-fat diet (HFD)-induced T2DM. Our experimental design employed 35 Guizhou mini-pigs to develop a model via HFD induction, aiming identify microbial and metabolic signatures associated pathogenesis resistance. At month 10, five individuals from the control (CTR), (DM), resistant (anti-DM) groups were slaughtered, samples collected, relevant indices measured. Metagenomics, metabolomics, 16S rRNA sequencing performed microbes metabolites linked progression Key findings demonstrated anti-DM group parameters-including (fasting blood glucose, insulin levels, HbA1c, IVGTT), histopathology (HE-stained pancreatic/hepatic tissues), profiles (structural, compositional, functional), metabolomic signatures-occupied intermediate positions between CTR DM groups. Network analyses revealed: (1) Lactobacillus , L. amylovorus fingolimod, polyoxyethylene sorbitan monooleate, thiamine, atrazine HFD-associated networks; (2) Limosilactobacillus reuteri N-oleoyl-L-serine, tolbutamide, tetradecanoyl carnitine, 3′-sulfogalactosylceramide, guggulsterone resistance (3) Ruminococcaceae NK4A214 diethyl phthalate, zingerone, enalapril, 5-hydroxytryptophol, 2′-deoxyinosine, icariin, emetine networks. These results further clarify role gut microbiota serum development mini-pig model.

Language: Английский

Paederia scandens extract alleviates obesity via modulating the gut microbiota and serum metabolome disorder

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 23, 2025

Obesity is increasingly becoming a challenge with China's economic development. There an urgent need to identify more affordable methods combat this condition. Paederia scandens (PS), cost-effective herbal remedy widely used in China for treating inflammation and pain, shows potential regard. To investigate its anti-obesity mechanisms, we established high-fat diet (HFD)-induced obesity model mice. The obese mice subsequently received daily oral gavage of PS extract 21 consecutive days. Upon the completion experiment, blood samples were collected analyze lipid profiles, including total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL-C), low-density (LDL-C). Abdominal adipose tissue was subjected hematoxylin-eosin (HE) staining histological analysis, while fecal underwent 16S rRNA sequencing assess gut microbiota composition. Our findings revealed that supplementation significantly reduced body weight, metabolism biomarkers, adipocyte size. treatment also restored microbial diversity, 19 specific taxa 25 KEGG pathways identified as mediators effects. Notably, modulated key obesity-associated microbiota, Alistipes, Lachnoclostridium, Odoribacter, Prevotellaceae UCG-001, Rikenellaceae RC9-gut group, norank_g Bacteroidales S24-7 group. Serum metabolomics analysis implicated L-ascorbic acid, stevioside, allopurinol, gingerol, along amino acid energy pathways, mechanism PS. These results provide novel theoretical insights into therapeutic prevention treatment.

Language: Английский