Oxidative Stress and Inflammation in Myocardial Ischemia–Reperfusion Injury: Protective Effects of Plant‐Derived Natural Active Compounds DOI
Jia Chen, Boyu Wang, Tianwei Meng

et al.

Journal of Applied Toxicology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

ABSTRACT Acute myocardial infarction (AMI) remains a leading cause of death among patients with cardiovascular diseases. Percutaneous coronary intervention (PCI) has been the preferred clinical treatment for AMI due to its safety and efficiency. However, research indicates that rapid restoration oxygen supply following PCI can lead secondary injury, termed ischemia–reperfusion injury (MIRI), posing grave threat patient survival. Despite ongoing efforts, mechanisms underlying MIRI are not yet fully elucidated. Among them, oxidative stress inflammation stand out as critical pathophysiological mechanisms, playing significant roles in MIRI. Natural compounds have shown strong therapeutic potential their high efficacy, availability, low side effects. Many current studies indicate natural mitigate by reducing inflammatory responses. Therefore, this paper reviews during role intervening these processes, aiming provide basis reference future development drugs treating

Language: Английский

A Mechanistic Overview on Green Assisted Formulation of Nanocomposites and Their Multifunctional Role in Biomedical Applications DOI Creative Commons

Mahnoor Mahnoor,

Khafsa Malik, Abeer Kazmi

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(3), P. e41654 - e41654

Published: Jan. 8, 2025

The importance of nanocomposites constantly attains attention because their unique properties all across the fields especially in medical perspectives. study green-synthesized has grown to be extremely fascinating field research. Nanocomposites are more promising than mono-metallic nanoparticles they exhibit synergistic effects. This review encapsulates current development formulation plant-mediated by using several plant species and impact secondary metabolites on biocompatible functioning. Phyto-synthesis produces diverse nanomaterials with biocompatibility, environment-friendliness, vivo actions, characterized varying sizes, shapes, biochemical nature. process is advantageous conventional physical chemical procedures. New studies have been conducted determine biomedical efficacy against various diseases. Unfortunately, there inadequate investigation into green-assisted nanocomposites. Incorporating phytosynthesized therapeutic interventions not only enhances healing processes but also augments host's immune defenses infections. highlights phytosynthesis applications, including antibacterial, antidiabetic, antiviral, antioxidant, antifungal, anti-cancer, other as well toxicity. explores mechanistic action achieve designated tasks. Biogenic for multimodal imaging potential exchange methods materials Well-designed utilized innovative theranostic agents subsequent objective efficiently diagnosing treating a variety human disorders.

Language: Английский

Citations

2
Tongmai Yangxin pill alleviates myocardial ischemia/reperfusion injury by regulating mitochondrial fusion and fission through the estrogen receptor alpha/peroxisome proliferator-activated receptor gamma coactivator-1 alpha signaling pathway DOI
Lu Yu,

Xu Wang,

Qian Lei

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119639 - 119639

Published: March 1, 2025

Language: Английский

Citations

1
Lipid Nanoparticle-Mediated Oip5-as1 Delivery Preserves Mitochondrial Function in Myocardial Ischemia/Reperfusion Injury by Inhibiting the p53 Pathway DOI
Xiaowei Niu, Jing Zhang, Jingjing Zhang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(45), P. 61565 - 61582

Published: Nov. 1, 2024

Myocardial ischemia/reperfusion (MI/R) injury, a major contributor to poor prognosis in patients with acute myocardial infarction, currently lacks effective therapeutic strategies clinical practice. The long noncoding RNA (lncRNA) Oip5-as1 can regulate various cellular processes, such as cell proliferation, differentiation, and survival. may have potential target for MI/R injury its upregulated expression has been associated reduced infarct size improved cardiac function animal models, although how effectively safely overexpress vivo remains unclear. Lipid nanoparticles (LNPs) are versatile technology targeted drug delivery numerous applications. Herein, we aimed assess the efficacy safety of LNPs coloaded cardiomyocyte-specific binding peptide (LNP@Oip5-as1@CMP) murine model injury. To achieve this, LNP@Oip5-as1@CMP was synthesized via ethanol injection method. structural components were physicochemically analyzed. A hypoxia/reoxygenation (H/R) HL-1 cells coronary artery ligation mice used simulate Our results demonstrated that designed cardiomyocyte targeting efficient successfully synthesized. In cells, treatment significantly mitochondrial apoptosis caused by H/R model, intravenous administration decreased function. Mechanistic investigations revealed inhibited p53 signaling pathway. However, cardioprotective effects abrogated administrating Nutlin-3a, activator. Furthermore, no signs organ damage detected after injection. study reveals mitigating These findings pave way advanced treatments cardiovascular diseases, emphasizing promise lncRNA-based therapies.

Language: Английский

Citations

6
The PINK1/Parkin signaling pathway-mediated mitophagy: a forgotten protagonist in myocardial ischemia/reperfusion injury DOI Creative Commons
Xiaopeng Zhao, Zheng Wang, Lijie Wang

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 209, P. 107466 - 107466

Published: Oct. 15, 2024

Myocardial ischemia causes extensive damage, further exacerbated by reperfusion, a phenomenon called myocardial ischemia/reperfusion injury (MIRI). Nowadays, the pathological mechanisms of MIRI have received attention. Oxidative stress, multiple programmed cell deaths, inflammation and others are all essential contributing to MIRI. Mitochondria energy supply centers cells. Numerous studies found that abnormal mitochondrial function is an "culprit" MIRI, mitophagy mediated phosphatase tensin homolog (PTEN)-induced kinase 1 (PINK1)/Parkin signaling pathway integral part maintaining function. Therefore, exploring association between PINK1/Parkin pathway-mediated crucial. This review will mainly summarize crucial role in MIR-induced several various potential interventions affect mitophagy, thus ameliorating

Language: Английский

Citations

4
Mangiferin Attenuates Myocardial Ischemia Reperfusion Injury by Regulating the GAS6/Axl Signaling Pathway DOI Open Access

Aizhen Zhao,

Wangrui Lei,

Jiayin Tian

et al.

Phytotherapy Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

ABSTRACT Ischemia reperfusion‐induced myocardial injury is a prominent pathological feature in patients with coronary artery disease, contributing to significant mortality and morbidity rates. Mangiferin (MGF), the main active ingredient extracted from Anemarrhena asphodeloides Bge , has anti‐inflammatory, anti‐oxidation, anti‐diabetes, anti‐tumor effects. The present study confirmed that GAS6/Axl pathway was identified as promising novel target for treatment of ischemia reperfusion (IR) injury. However, whether MGF exerts anti‐myocardial through still unclear. In this study, BALB/c male mice HL‐1 cardiomyocytes were used construct model IR hypoxia‐reoxygenation (HR) (or H 2 O ) vivo vitro, respectively. significantly improved cardiac function indicators, structure, enzymes, mitochondrial function, together reduced oxidative stress apoptosis IR‐injured mice. increased cell viability, inhibited release LDH, apoptosis, both HR ‐injured cells. particular, signaling plays an important role process. Additionally, we also demonstrated GAS6 gene knockout reversed protective effect against cardiomyocytes. effects by activating pathway, providing theoretical basis potential cardioprotective drug clinical setting

Language: Английский

Citations

0
RGS12 is a target of penehyclidine hydrochloride that enhances oxidative stress and ferroptosis in a model of myocardial ischemia/reperfusion injury by inhibiting the Nrf2 pathway DOI Creative Commons

Congna Zi,

Yulei Wei, Ying Zhu

et al.

International Journal of Molecular Medicine, Journal Year: 2025, Volume and Issue: 55(3)

Published: Jan. 24, 2025

Regulator of G‑protein signaling 12 (RGS12) is a regulatory factor that involved in various physiological processes. However, the role RGS12 myocardial ischemia/reperfusion injury (MIRI) currently remains unclear. The present study established mouse model MIRI by ligating left main coronary artery followed reperfusion. In addition, HL‑1 cells were cultured hypoxic and serum‑free medium, reoxygenation to establish an in vitro cell hypoxia/reoxygenation (H/R). Adenoviruses targeting subsequently used either overexpress or silence expression. was highly expressed both tissues mice with subjected H/R. results from experiments demonstrated knockdown reduced oxidative stress under pathological environment, as indicated decreased reactive oxygen species (ROS) levels malondialdehyde activity increased activities superoxide dismutase catalase. Furthermore, underwent H/R stimulation exhibited ferroptosis, whereas reversed these changes. These showed post‑RGS12 silencing Fe2+ lipid ROS decreased, expression glutathione peroxidase 4 cystine transporter solute carrier family 7 member 11 mitochondrial structure improved preventing loss crest. Mechanistically, nuclear erythroid 2‑related 2 (Nrf2) pathway anti‑ferroptosis anti‑oxidative capacities activated knockdown. Conversely, overexpression exerted opposite effects vivo vitro. Notably, it penehyclidine hydrochloride (PHC), known block process, vivo vitro, inhibited therapeutic PHC on MIRI. conclusion, RGS12, target PHC, potentially enhanced progression promoting this effect may involve regulation Nrf2 pathway.

Language: Английский

Citations

0
M6a demethylase FTO regulates the oxidative stress, mitochondrial biogenesis of cardiomyocytes and PGC-1a stability in myocardial ischemia-reperfusion injury DOI Creative Commons
Qiong Jiang, Xuehai Chen,

Kezeng Gong

et al.

Redox Report, Journal Year: 2025, Volume and Issue: 30(1)

Published: Jan. 27, 2025

Myocardial ischemia-reperfusion injury (MIRI) is a highly complex disease with high morbidity and mortality. Studying the molecular mechanism of MIRI discovering new targets are crucial for future treatment MIRI. We constructed rat model hypoxia/reoxygenation (H/R) cardiomyocytes model. RT-PCR Western blot were used to investigate expression fat mass obesity-associated (FTO) gene. Electrocardiogram, echocardiography, triphenyltetrazolium chloride (TTC) staining hematoxylin-eosin (HE) assess effect FTO overexpression. The generation reactive oxygen species (ROS) levels superoxide dismutase (SOD2), mitochondrial transcription factor (TFAM) cytochrome c oxidase I (COXI) detected oxidative stress biogenesis. RNA immunoprecipitation (RIP) pulldown assays identify interaction PGC-1a. m6A dot blot, methylated PCR (MeRIP-PCR) stability analysis analyze regulation methylation PGC-1a by FTO. was downregulated in rats H/R induced cardiomyocytes. Overexpression inhibited ROS level increased SOD2, TFAM COXI vitro vivo. In addition, identified as downstream target enhanced mRNA through removing modification. Our study revealed role regulates biogenesis via MIRI, which may provide approach mitigating

Language: Английский

Citations

0
Ligustilide covalently binds to Cys254 of the creatine kinase, M-type protein, ameliorating acute myocardial ischemia by enhancing the creatine phosphate level DOI

Kaixue Zhang,

Guoqing Luan,

Jing Zhang

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 139, P. 156532 - 156532

Published: Feb. 18, 2025

Language: Английский

Citations

0
Apelin13 Loaded Nano-Niosomes Confer Cardioprotection in a Rat Model of Myocardial Ischemia Reperfusion by Targeting the Nrf2/HO-1 Pathway DOI

Neda Tekiyeh Maroof,

Saeed Mehrzadi, Maryam Naseroleslami

et al.

Journal of Cardiovascular Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

Citations

0
α-Cyperone Alleviates LPS-Induced Pyroptosis in Rat Aortic Endothelial Cells via the PI3K/AKT Signaling Pathway DOI Creative Commons
Shuanghui Liu,

Yankun Zhang,

Xiaoxia Liang

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 303 - 303

Published: Feb. 22, 2025

Objective: To investigate the effect and underlying mechanism of α-cyperone in inhibiting pyroptosis rat aortic endothelial cells (RAECs). Methods: Molecular docking technology was used to predict potential binding affinity pyroptosis-related proteins. A model established RAECs using serum containing 10% LPS, with administered as a preventive treatment for 9 h. Cell viability membrane integrity were assessed propidium iodide (PI) staining CCK-8 assay. The release IL-1β IL-18 quantified by ELISA. Western blot RT-qPCR performed evaluate expression levels NLRP3, ASC, caspase-1 p20, N-GSDMD. Additionally, RNA sequencing analysis conducted identify differentially expressed genes related LPS-induced following treatment, key differential validated blot. Results: reveals that exhibits strong targets. α-Cyperone significantly improves cell (p < 0.001), reduces downregulates mRNA protein caspase-1, GSDMD 0.001). indicates primarily modulates gene through PI3K/AKT signaling pathway. validation further confirmed effectively inhibited phosphorylated total PI3K AKT Conclusions: alleviates morphological damage RAEC model, suppresses proinflammatory cytokines IL-18, potentially inhibits NLRP3/caspase-1/GSDMD activation pathway, thereby attenuating RAECs.

Language: Английский

Citations

0