Single-Cell Sequencing Combined with Transcriptome Sequencing to Explore the Molecular Mechanisms Related to Skin Photoaging DOI Creative Commons
Xinru Hu, Shuang Du, Meng Chen

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 11137 - 11160

Published: Dec. 1, 2024

Background:The aging of skin is a diversified biological phenomenon, influenced by combination genetic and environmental factors.However, the specific mechanism photoaging not yet completely elucidated.Methods: Gene expression profiles for patients were obtained from Expression Omnibus (GEO) collection.We conducted single-cell intercellular communication investigations to identify potential gene sets.Predictive models created using LASSO regression.The relationships between genes immune cells investigated single sample set enrichment analysis (ssGSEA) variance (GSVA).The molecular processes important studied analysis.A miRNA network was look target miRNAs connected with genes, transcriptional regulation used related transcription factors.Finally, merging co-expression networks drug prediction shows pathways treatment targets.Furthermore, we validated role key cell infiltration, Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) pathway in photoaging, which identified through bioinformatics analysis, vivo reverse quantitative PCR (RT-qPCR), immunofluorescence labeling, Western blotting.Results: This study discovered three including Atp2b1, Plekho2, Tspan13, perform crucial functions process.Immune infiltration showed increased M1 macrophages CD4 memory T group.Further signaling indicated that these are enriched multiple metabolic pathways.The significant roles AMPK vivo. Conclusion:This research revealed underlying mechanisms indicating such as Atp2b1 Tspan13 play pathways.These findings provide new theory prospective targets advancement relevant drugs.

Language: Английский

Efficacy and Safety of Orally Administered East Asian Herbal Medicine Combined with Narrowband Ultraviolet B against Psoriasis: A Bayesian Network Meta-Analysis and Network Analysis DOI Open Access
Hee-Geun Jo, Hyehwa Kim, Eunhye Baek

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(16), P. 2690 - 2690

Published: Aug. 13, 2024

Psoriasis is a chronic, immune-mediated inflammatory skin disease with many complications and poor prognosis that imposes significant burden on individuals society. Narrowband ultraviolet B (NB-UVB) represents cost-effective non-drug therapeutic intervention for psoriasis. East Asian herbal medicine (EAHM) currently being investigated its potential as safe effective psoriasis treatment. Consequently, it has the to be employed combination therapy NB-UVB. The objective was ascertain efficacy safety of EAHM NB-UVB identify important drugs further research. In this study, randomized controlled trials (RCTs) were retrieved from ten databases in Korea, China, Japan. All statistical analyses conducted using R software version 4.3.0. primary outcomes Area Severity Index (PASI) incidence rate adverse events (AEs), while secondary hematologic markers Dermatology Life Quality (DLQI), which reflect pathology analysis 40 RCTs, including 3521 participants, demonstrated exhibited statistically superiority over monotherapy respect outcomes. Bayesian network meta-analysis revealed Investigator Presciption 3 Ziyin Liangxue Decoction consistent relative advantage each PASI-based metric. estimated influence ranking all individual herbs according PageRank centrality. findings study suggest EAHMs co-administered may provide additional safety-related benefits patients However, quality evidence still low, high-quality are needed reach more definitive conclusions.

Language: Английский

Citations

1
Integrated Multispectral and Multilevel Data Optimization for Rapid Origin Tracing and Quality Assessment of Salvia Miltiorrhiza DOI
Rao Fu, Chen Peng, Jia Qiao

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Unveiling ferroptosis: a new frontier in skin disease research DOI Creative Commons
Ke Wang,

Yumeng Lin,

Dan Zhou

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 4, 2024

Ferroptosis, a form of regulated cell death distinct from apoptosis, necrosis, and autophagy, is increasingly recognized for its role in skin disease pathology. Characterized by iron accumulation lipid peroxidation, ferroptosis has been implicated the progression various conditions, including psoriasis, photosensitive dermatitis, melanoma. This review provides an in-depth analysis molecular mechanisms underlying compares cellular effects with other forms context health disease. We systematically examine five specific diseases, ichthyosis, polymorphous light eruption (PMLE), vitiligo, melanoma, detailing influence on pathogenesis progression. Moreover, we explore current clinical landscape ferroptosis-targeted therapies, discussing their potential managing treating diseases. Our aim to shed therapeutic modulating research practice.

Language: Английский

Citations

0
Anti-inflammatory and antioxidant succinyl-chitosan oligosaccharide protects human epidermal cell and mouse skin against ultraviolet B-induced photodamage DOI

Xiansen Lv,

Zi Han,

Huyan Huo

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 351, P. 123102 - 123102

Published: Dec. 3, 2024

Language: Английский

Citations

0
Single-Cell Sequencing Combined with Transcriptome Sequencing to Explore the Molecular Mechanisms Related to Skin Photoaging DOI Creative Commons
Xinru Hu, Shuang Du, Meng Chen

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 11137 - 11160

Published: Dec. 1, 2024

Background:The aging of skin is a diversified biological phenomenon, influenced by combination genetic and environmental factors.However, the specific mechanism photoaging not yet completely elucidated.Methods: Gene expression profiles for patients were obtained from Expression Omnibus (GEO) collection.We conducted single-cell intercellular communication investigations to identify potential gene sets.Predictive models created using LASSO regression.The relationships between genes immune cells investigated single sample set enrichment analysis (ssGSEA) variance (GSVA).The molecular processes important studied analysis.A miRNA network was look target miRNAs connected with genes, transcriptional regulation used related transcription factors.Finally, merging co-expression networks drug prediction shows pathways treatment targets.Furthermore, we validated role key cell infiltration, Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) pathway in photoaging, which identified through bioinformatics analysis, vivo reverse quantitative PCR (RT-qPCR), immunofluorescence labeling, Western blotting.Results: This study discovered three including Atp2b1, Plekho2, Tspan13, perform crucial functions process.Immune infiltration showed increased M1 macrophages CD4 memory T group.Further signaling indicated that these are enriched multiple metabolic pathways.The significant roles AMPK vivo. Conclusion:This research revealed underlying mechanisms indicating such as Atp2b1 Tspan13 play pathways.These findings provide new theory prospective targets advancement relevant drugs.

Language: Английский

Citations

0