Effect of macrophage‐to‐myofibroblast transition on silicosis DOI Creative Commons

Fei Geng,

Jingrou Xu,

Xichen Ren

et al.

Animal Models and Experimental Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: July 9, 2024

Abstract Background The aim was to explore the effect of macrophage polarization and macrophage‐to‐myofibroblast transition (MMT) in silicosis. Methods Male Wistar rats were divided into a control group silicosis developed using HOPE MED 8050 dynamic automatic dusting system. Murine MH‐S cells randomly an SiO 2 group. pathological changes lung tissue observed hematoxylin eosin (HE) Van Gieson (VG) staining. distribution location marker (F4/80), M1 (iNOS), M2 (CD206), myofibroblast (α‐smooth muscle actin [α‐SMA]) detected immunohistochemical immunofluorescent expression iNOS, Arg, α‐SMA, vimentin, type I collagen (Col I) measured Western blot. Results results HE VG staining showed obvious silicon nodule formation thick fibers Macrophage F4/80 increased gradually from 8 32 weeks after exposure silica. Immunohistochemical revealed that there more iNOS‐positive some CD206‐positive at weeks. More found nodules tissues blot analysis expressions Inducible nitric oxide synthase Arg protein upregulated compared with those immunofluorescence co‐expression F4/80, Col I, CD206 α‐SMA co‐expressed extracted rat alveolar lavage fluid + , Similar also induced by . Conclusions development is accompanied MMT.

Language: Английский

Chinese and western medicine treatment of myocardial fibrosis drugs DOI Creative Commons
Yuxi Zhu, Fangmei Zhang, Zhongcheng Li

et al.

Frontiers in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 15, 2025

Myocardial fibrosis (MF) is a common pathological manifestation of many cardiovascular diseases, such as myocardial infarction, ischemia, and sudden cardiac death. It characterized by excessive proliferation activation fibroblasts, transformation into myofibroblasts, and, eventually, deposition the extracellular matrix, resulting in heart damage. Currently, modern drugs angiotensin-converting enzyme inhibitors, diuretics, β-blockers can improve clinical treatment, but their therapeutic effect on this disease limited, with obvious side effects high cost. Traditional Chinese medicine (TCM) has advantages multiple targets, low cost, few effects. medicines, Salvia miltiorrhiza, Astragalus, Angelica extracts, patent Qiliqiangxin capsules, Shenqi Yiqi dropping pills, Tongxinluo fibrosis. In review, current Western methods for treating are discussed. The signaling pathways targets involved treatment This review aimed to provide valuable insights ideas both basic research

Language: Английский

Citations

0
Integrated serum metabolomics and network pharmacology reveal molecular mechanism of Qixue Huazheng formula on peritoneal fibrosis DOI Creative Commons

Xiaohui Meng,

Sheng Li, Yongqing You

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 23, 2025

Background Peritoneal fibrosis (PF) causes peritoneal dialysis (PD) withdrawal due to ultrafiltration failure. Qixue Huazheng formula (QXHZF), comprising Astragalus membranaceus , Centella asiatica and Ligusticum sinense is applied treat PD-related peritoneum injury related; however, the active components, core genes, underlying mechanism involved remain unclear. Methods The anti-PF effects of QXHZF were verified in vivo vitro . Targets QXHZF-mediated improvement PD-induced PF predicted using network pharmacology analysis. Metabolites associated with treatment analyzed by serum metabolomics. Integration metabolomics findings identified potentially important pathways, metabolites, targets, molecular docking studies confirmed interactions key components targets. Western blotting (WB), quantitative real-time PCR (qRT-PCR), TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, flow cytometry conducted. Results had potent therapeutic efficacy against according WB, qRT-PCR, pathological section examination. Network pharmacological analysis indicated that multiple compounds contributed improving modulating various targets pathways. Differential metabolites Integrated data steroid hormone biosynthesis, Ras signaling pathway, apoptosis, estrogen QXHZF. Metabolite-target analyses revealed can bind receptor 1 (ESR1) rapidly accelerated fibrosarcoma (RAF1) through its components. WB demonstrated reversed activation above-mentioned thereby inhibiting PD fluid-induced PF. Conclusion significantly ameliorate may regulate signaling, apoptosis this context.

Language: Английский

Citations

0
Astragaloside IV as a promising therapeutic agent for liver diseases: current landscape and future perspectives DOI Creative Commons
Chunyan Chen,

Xuexian Bu,

Liping Deng

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 23, 2025

Astragaloside IV (C41H68O14, AS-IV) is a naturally occurring saponin isolated from the root of Astragalus membranaceus, widely used traditional Chinese botanical drug in medicine. In recent years, AS-IV has attracted considerable attention for its hepatoprotective properties, which are attributed to low toxicity as well anti-inflammatory, antioxidant and antitumour effects. Numerous preclinical studies have demonstrated potential prevention treatment various liver diseases, including multifactorial injury, metabolic-associated fatty disease, fibrosis cancer. Given promising growing interest research, this review provides comprehensive summary current state research on effects AS-IV, based literature available databases such CNKI, PubMed, ScienceDirect, Google Scholar Web Science. The mechanisms multifaceted, encompassing inhibition inflammatory responses, reduction oxidative stress, improvement insulin leptin resistance, modulation gut microbiota, suppression hepatocellular carcinoma cell proliferation induction tumour apoptosis. Notably, key molecular pathways involved these include Nrf2/HO-1, NF-κB, NLRP3/Caspase-1, JNK/c-Jun/AP-1, PPARα/FSP1 Akt/GSK-3β/β-catenin. Toxicity indicate that high level safety. addition, discusses sources, physicochemical challenges development clinical application providing valuable insights into agent pharmaceutical nutraceutical industries.

Language: Английский

Citations

0
Exosomes from renal cells and macrophages: Bidirectional communication in the pathogenesis of kidney disease DOI
Jing Gao, Pan Chen, Wenjing Zhao

et al.

Cytokine, Journal Year: 2025, Volume and Issue: 192, P. 156961 - 156961

Published: May 17, 2025

Language: Английский

Citations

0
BuyangHuanwu Decoction alleviates Endothelial Cell Apoptosis and Coronary Microvascular Dysfunction via Regulation of the MAPKK4/p38 Signaling Axis DOI Creative Commons

Xing Chang,

Dan Wu, Xin Gao

et al.

International Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 21(13), P. 2464 - 2479

Published: Jan. 1, 2024

MAPKK4 has been implicated in the pathological mechanisms underlying myocardial and vascular injury, specifically influencing endothelial cell damage programmed death via subcellular pathways. Nevertheless, regulatory role of coronary microvascular injury following infarction remains unconfirmed, exploration targeted mitochondrial protective therapeutic agents unaddressed. In light this gap, we established a gene-modified mouse model ischemia-reperfusion employed Buyang Huanwu decoction (BYHW), traditional cardiovascular formula, to assess its efficacy treating post-ischemia-reperfusion. The study aimed elucidate mechanism by which BYHW mitigates induced through attenuation apoptosis. Experimental outcomes revealed that high-dose significantly ameliorated post-ischemia-reperfusion, restoring structural integrity microvasculature reducing inflammation oxidative stress. Contrarily, transgenic mice overexpressing MAPKK4, intervention failed attenuate To further investigate, simulated hypoxia/reoxygenation cells using MAPKK4-related cellular gene modification model. results indicated attenuates inflammatory enhances viability hypoxic stress, inhibiting apoptosis pathway. However, overexpression MAPKK4/p38 negated effects BYHW, showing no impact on stress under conditions. Molecular interaction studies confirmed active components Astragaloside IV Ligustrazine, interact with MAPKK4/P38 axis.

Language: Английский

Citations

2
Effect of macrophage‐to‐myofibroblast transition on silicosis DOI Creative Commons

Fei Geng,

Jingrou Xu,

Xichen Ren

et al.

Animal Models and Experimental Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: July 9, 2024

Abstract Background The aim was to explore the effect of macrophage polarization and macrophage‐to‐myofibroblast transition (MMT) in silicosis. Methods Male Wistar rats were divided into a control group silicosis developed using HOPE MED 8050 dynamic automatic dusting system. Murine MH‐S cells randomly an SiO 2 group. pathological changes lung tissue observed hematoxylin eosin (HE) Van Gieson (VG) staining. distribution location marker (F4/80), M1 (iNOS), M2 (CD206), myofibroblast (α‐smooth muscle actin [α‐SMA]) detected immunohistochemical immunofluorescent expression iNOS, Arg, α‐SMA, vimentin, type I collagen (Col I) measured Western blot. Results results HE VG staining showed obvious silicon nodule formation thick fibers Macrophage F4/80 increased gradually from 8 32 weeks after exposure silica. Immunohistochemical revealed that there more iNOS‐positive some CD206‐positive at weeks. More found nodules tissues blot analysis expressions Inducible nitric oxide synthase Arg protein upregulated compared with those immunofluorescence co‐expression F4/80, Col I, CD206 α‐SMA co‐expressed extracted rat alveolar lavage fluid + , Similar also induced by . Conclusions development is accompanied MMT.

Language: Английский

Citations

0