Investigation of bioactive components and metabolic pathways of Zhen‐wu‐tang in rat plasma and renal tissue by UPLC‐Q‐TOF/MS DOI

Shengliang Yuan,

Junqi Chen, Yiwen Cao

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract Introduction Zhen‐wu‐tang (ZWT) is a traditional Chinese medicine (TCM) formula for the treatment of several kidney diseases. However, due to complexity TCM formula, there lack accurate knowledge chemical constituents ZWT and its bioactive components, as well in vivo metabolic pathway studies. Objectives The composition components along with pathways were investigated by combination profiling serum pharmacochemistry. Methods High‐resolution ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry was used identify metabolites vivo. Results As result, total 110 identified from solution, mainly amino acids, alkaloids, gingerols, monoterpene glycosides terpenoids, so on. In addition, 24 prototype 36 detected rat plasma. Meanwhile, 8 tissue but no metabolites. Interestingly, 4 28 both plasma renal tissue, which atractylenolide III, trimethoxyaconitane, methyl gallate, paeoniflorin. involved Phases I and/or II reactions such hydrolysis, oxidation, reduction hydration, methylation/demethylation, sulphation, glucuronidation, acetylation, glutathione conjugation. Conclusion Overall, present study has comprehensively elucidated potential metabolites, provides basis basic pharmacodynamic substances reference formulae.

Language: Английский

Regulation of macrophage polarization by metformin through inhibition of TLR4/NF-κB pathway to improve pre-eclampsia DOI

Wei Shen,

Qingfu Wang, Guofang Shen

et al.

Placenta, Journal Year: 2025, Volume and Issue: 160, P. 89 - 99

Published: Jan. 5, 2025

Language: Английский

Citations

0
Tetrahydropalmatine ameliorates peripheral nerve regeneration by enhancing macrophage anti-inflammatory response DOI
Yongsheng Jiang,

Jianye Cao,

Rui Li

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 147, P. 114000 - 114000

Published: Jan. 6, 2025

Language: Английский

Citations

0
Prdx5 regulates macrophage polarization by modulating the TLR4/NF-κB pathway to promote apoptosis in chronic prostatitis DOI
Weikang Wu,

Meng Tong,

Yufan Wang

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 151, P. 114332 - 114332

Published: Feb. 26, 2025

Language: Английский

Citations

0
Stachyose ameliorates myocardial ischemia–reperfusion injury by inhibiting cardiomyocyte ferroptosis and macrophage pyroptosis DOI

Ao-Yuan Zhang,

Jia‐Bao Su,

Heting Sun

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 143, P. 113334 - 113334

Published: Oct. 8, 2024

Language: Английский

Citations

3
Investigation of bioactive components and metabolic pathways of Zhen‐wu‐tang in rat plasma and renal tissue by UPLC‐Q‐TOF/MS DOI

Shengliang Yuan,

Junqi Chen, Yiwen Cao

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract Introduction Zhen‐wu‐tang (ZWT) is a traditional Chinese medicine (TCM) formula for the treatment of several kidney diseases. However, due to complexity TCM formula, there lack accurate knowledge chemical constituents ZWT and its bioactive components, as well in vivo metabolic pathway studies. Objectives The composition components along with pathways were investigated by combination profiling serum pharmacochemistry. Methods High‐resolution ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry was used identify metabolites vivo. Results As result, total 110 identified from solution, mainly amino acids, alkaloids, gingerols, monoterpene glycosides terpenoids, so on. In addition, 24 prototype 36 detected rat plasma. Meanwhile, 8 tissue but no metabolites. Interestingly, 4 28 both plasma renal tissue, which atractylenolide III, trimethoxyaconitane, methyl gallate, paeoniflorin. involved Phases I and/or II reactions such hydrolysis, oxidation, reduction hydration, methylation/demethylation, sulphation, glucuronidation, acetylation, glutathione conjugation. Conclusion Overall, present study has comprehensively elucidated potential metabolites, provides basis basic pharmacodynamic substances reference formulae.

Language: Английский

Citations

1