Identification of compounds against atherosclerosis induced by ox-LDL based on cell extraction/UPLC–MS/MS from mulberry twigs and their mechanistic analysis
Chemical and Biological Technologies in Agriculture,
Journal Year:
2025,
Volume and Issue:
12(1)
Published: Jan. 15, 2025
Mulberry
twigs,
a
traditional
Chinese
medicinal
and
agricultural
byproduct,
contain
bioactive
compounds
with
anti-atherosclerotic
potential.
This
study
aims
to
identify
evaluate
the
effects
of
key
in
mulberry
twig
extracts
(MTEs)
on
oxidized
low-density
lipoprotein
(ox-LDL)-induced
human
umbilical
vein
endothelial
cells
(HUVECs),
focus
understanding
how
these
modulate
oxidative
stress
related
signaling
pathways.
Biospecific
cell
extraction
ultra-performance
liquid
chromatography–tandem
mass
spectrometry
(UHPLC–MS/MS)
were
employed
screen
compounds.
Protective
assessed
by
measuring
viability,
malondialdehyde
(MDA),
superoxide
dismutase
(SOD)
levels,
along
detecting
intracellular
reactive
oxygen
species
(ROS)
using
2,
7-dichlorodihydrofluorescein
diacetate
(DCFH-DA)
dihydroethidine
(DHE)
probes.
Real-time
qPCR
Western
blotting
used
for
mRNA
protein
level
analysis.
Two
novel
active
compounds,
Kuwanon
H
Morusin,
known
G,
identified.
They
significantly
reduced
MDA
ROS
levels
while
increasing
SOD
activity
ox-LDL-treated
HUVECs.
was
particularly
effective,
enhancing
nuclear
factor
erythroid
2-related
2
(Nrf-2)
upregulating
its
target
genes
Heme
oxygenase-1
(HO-1)
NAD(P)H:
quinone
oxidoreductase
1
(NQO-1).
In
conclusion,
H,
G
effectively
protected
HUVECs
from
ox-LDL-induced
injury,
showing
strongest
protective
via
Nrf-2/HO-1
pathway.
These
hold
potential
treating
atherosclerosis
diseases.
Language: Английский
Lithospermic acid alleviates oxidative stress and inflammation in DSS-induced colitis through Nrf2
Yu-Lei Zhao,
No information about this author
Xue Tian,
No information about this author
Yan Yao
No information about this author
et al.
European Journal of Pharmacology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 177390 - 177390
Published: Feb. 1, 2025
Language: Английский
A novel galactoglucan from Ganoderma lucidum ameliorates ethanol-induced gastric ulcers by modulating FAK-MAPK signaling pathway
Hongjian Luo,
No information about this author
Yukun Zhang,
No information about this author
Yang Yao
No information about this author
et al.
Carbohydrate Polymers,
Journal Year:
2025,
Volume and Issue:
unknown, P. 123594 - 123594
Published: April 1, 2025
Language: Английский
Escin Ia Ameliorates DSS-Induced Chronic Colitis in Mice by Inhibiting Inflammation and Oxidative Stress via the LOXL2/MMP-9 Pathway
Journal of Ethnopharmacology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 119623 - 119623
Published: March 1, 2025
Language: Английский
New insights into the intestinal barrier through “gut-organ” axes and a glimpse of the microgravity’s effects on intestinal barrier
Hong‐Yun Nie,
No information about this author
Jun Ge,
No information about this author
Guo-Xing Huang
No information about this author
et al.
Frontiers in Physiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Oct. 10, 2024
Gut
serves
as
the
largest
interface
between
humans
and
environment,
playing
a
crucial
role
in
nutrient
absorption
protection
against
harmful
substances.
The
intestinal
barrier
acts
initial
defense
mechanism
non-specific
infections,
with
its
integrity
directly
impacting
homeostasis
health
of
human
body.
primary
factor
attributed
to
impairment
previous
studies
has
always
centered
on
gastrointestinal
tract
itself.
In
recent
years,
concept
"gut-organ"
axis
gained
significant
popularity,
revealing
profound
interconnection
gut
other
organs.
It
speculates
that
disruption
these
axes
plays
pathogenesis
progression
damage.
evaluation
function
detection
enterogenic
endotoxins
can
serve
"detecting
agents"
for
identifying
early
functional
alterations
heart,
kidney,
liver,
thereby
facilitating
timely
intervention
disorders.
Simultaneously,
consolidating
may
also
present
potential
therapeutic
approach
attenuate
damage
Studies
have
demonstrated
diverse
signaling
pathways
their
corresponding
key
molecules
are
extensively
involved
pathophysiological
regulation
barrier.
Aberrant
activation
dysregulated
expression
play
pivotal
process
impairment.
Microgravity,
being
predominant
characteristic
space,
potentially
exert
influence
barriers.
We
will
discuss
interaction
damage,
further
elucidate
underlying
summarize
various
components
under
microgravity.
This
review
aims
offer
novel
perspective
comprehending
etiology
molecular
mechanisms
injury
well
prevention
management
microgravity
environment.
Language: Английский
Chitosan oligosaccharide alleviates DON-induced liver injury via suppressing ferroptosis in mice
Ecotoxicology and Environmental Safety,
Journal Year:
2024,
Volume and Issue:
290, P. 117530 - 117530
Published: Dec. 13, 2024
Language: Английский
Chemical Inhibition of NRF2 Transcriptional Activity Influences Colon Function and Oestrogen Receptor Expression in Mice at Different Ages
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(24), P. 13647 - 13647
Published: Dec. 20, 2024
We
aim
to
investigate
whether
chemical
inhibition
of
NRF2
transcriptional
activity
(TA)
influences
distal
colon
contractions,
particularly
in
an
age-dependent
manner
females,
and
it
impacts
oestrogen
receptor
signalling
female
mice.
This
study
was
performed
on
3
6-month-old
mice
treated
with
ML385
(30
mg/kg)
or
a
vehicle
for
7
days
(i.p.).
The
functionality
verified
bead
expulsion
test;
serum
samples
were
collected
oestradiol
levels,
stored
various
histological
analyses.
results
show
that
the
seven-day
treatment
significantly
downregulated
TA
(p
<
0.05)
impacted
its
contractility.
Additionally,
young
females
exhibited
increase
goblet
cell
number
increased
ERα,
but
not
ERβ,
especially
older
It
is
worth
noting
basal
level
membrane
GPR30
higher
within
epithelial
layer,
led
downregulation
In
summary,
decreases
contractility
numbers.
expression
receptors
colons
Language: Английский