Preparation and analysis of quinoa active protein (QAP) and its mechanism of inhibiting Candida albicans from a transcriptome perspective

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18961 - e18961

Published: Feb. 14, 2025

The globe cultivation and industrial production of quinoa have been steadily increasing. Nevertheless, the full potential quinoa’s nutritional economic benefits remains underexploited. This study investigates isolation purification active protein (QAP) through precipitation method using ammonium sulfate from a phosphate extraction solution. process involved gel filtration chromatography with Sephadex G-75 G-50 columns to obtain QAP fractions exhibiting inhibitory effects against Candida albicans ( C. ). A comprehensive series experiments was undertaken examine antifungal properties these fractions. Proteomic analysis employed elucidate composition proteins. Furthermore, activities succinate dehydrogenase, Ca 2+ -Mg -ATPase, catalase in following treatment were quantified an enzyme-linked immunosorbent assay. on cell morphology cultured Spider agar medium further investigated scanning electron microscopy (SEM). RNA-seq conducted investigate alterations gene expression cells subjected treatment. To functional significance changes, Gene Ontology Kyoto Encyclopedia Genes Genomes analyses performed. Quantitative real-time polymerase chain reaction subsequently validate observed changes expression. Our findings demonstrate that exhibits , minimum concentration 182 µg/mL. Through proteomic analysis, definitive set 18 proteins identified. At molecular level, genes associated hyphal development, wall membrane integrity, cellular respiration, energy metabolism found be enriched. Protein–protein interaction (PPI) revealed inhibit growth hyphae, compromise suppress oxidative phosphorylation. These disruptions collectively impair normal metabolic activities, thereby exerting effect .

Language: Английский

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Untargeted metabolomics reveals biochemical changes in wheat by-products during fermentation

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106158 - 106158

Published: Feb. 1, 2025

Language: Английский

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Tropical lakes as a novel source of oleaginous yeasts with lipid profiles for biodiesel, oleochemical, and nutraceutical applications

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(3)

Published: March 1, 2025

Abstract Oleaginous yeasts have emerged as promising microbial cell factories for lipid production, offering sustainable alternatives to traditional sources of biodiesel and nutraceuticals. In this study, the accumulation potential yeast strains isolated from two freshwater aquatic ecosystems in Cali, Colombia, was evaluated identify novel candidates biotechnological applications. A total 56 were tested their oleaginous nature using a gravimetric assay with glucose carbon source. Of assessed strains, 46.15% exceeded 20% yields relative dry biomass. Seven selected glycerol source, but only five further characterized profiles. Molecular identification revealed diverse species, including Aureobasidium sp., Papiliotrema rajashtanensis, Rhodotorula spp., Clavispora lusitaniae . The demonstrated unique profiles, high proportions monounsaturated polyunsaturated fatty acids, such oleic acid (C18:1) linoleic (C18:2). particular, sp. accumulated uncommon acids petroselinic under conditions induced by glycerol. This acid, which has double bond position 6,7 melting point 33 °C, highlights its an alternative margarine well precursor sophorolipids, estolide esters, soaps, plastics. exhibited very long-chain docosadienoic docosatrienoic profile. These findings underscore value lentic systems producers, paving way innovations biofuels, nutraceuticals, oleochemicals.

Language: Английский

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Cornua cervi degelatinatum inhibits breast cancer stem-like cell properties and metastasis via miR-148a-3p-mediated TGF-β/Smad2 pathway

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119709 - 119709

Published: March 1, 2025

Language: Английский

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