The biological activity and potential of probiotics-derived extracellular vesicles as postbiotics in modulating microbiota-host communication DOI Creative Commons
Xiaoming Zhang, Ye Wang,

E Qiyu

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: May 16, 2025

Probiotics such as Lactobacillus and Bifidobacterium spp. have been shown to be critical for maintaining host homeostasis. In recent years, key compounds of postbiotics derived from probiotic metabolism cellular secretion identified their role in organ immunity regulating intestinal inflammation. particular, probiotic-derived extracellular vesicles (PEVs) can act postbiotics, almost the same functional activity probiotics. They also strong biocompatibility loading capacity carry exogenous or parental active molecules reach distal organs play roles. This provides a new direction understanding intrinsic microbiota-host communication mechanism. However, most current studies on PEVs are limited effects/benefits, specific physicochemical properties, composition, mechanisms homeostasis, possible threats remain explored. Here, we review summarize unique properties bioactivities mediating communication, elucidate limitations research potential application postbiotics.

Language: Английский

The Anti-Inflammatory Effects of Formononetin, an Active Constituent of Pueraria montana Var. Lobata, via Modulation of Macrophage Autophagy and Polarization DOI Creative Commons

Linyi Xu,

Shuo Zhou, Jing Li

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(1), P. 196 - 196

Published: Jan. 6, 2025

Pueraria montana var. lobata (Willd.) Maesen & S.M.Almeida ex Sanjappa Predeep (P. lobata) is a medicinal herb widely used in the food and pharmaceutical industries, studies have shown that P. possesses significant anti-inflammatory pharmacological activities. In this paper, total of 16 compounds were isolated identified from lobata, among which 1–3, 7, 14, for first time. The results an vitro activity screening assay showed 1, 4, 6, 8, 15 able to significantly reduce levels pro-inflammatory cytokines IL-6 IL-1β LPS-induced RAW264.7 macrophages, with most obvious effect produced by compound 6 (formononetin), while formononetin was number macrophages at site inflammation transgenic zebrafish. addition, network analysis revealed closely related autophagy polarization targets such as TNF, EGFR, PTGS2, ESR1. validation experiments could enhance expression LCII/LCI P62 protein, CD86, CD206, further indicated regulating macrophage processes.

Language: Английский

Citations

1
Big lessons from the little Akkermansia muciniphila in hepatocellular carcinoma DOI Creative Commons
Yanguang Yang, Xinli Shi

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 14, 2025

Hepatocellular carcinoma (HCC) is the most frequently occurring type of liver tumor and considered one common primary malignant neoplasms. The prognosis for HCC dismal because its complicated etiology high level medication resistance. Immunotherapy presently regarded as effective therapeutic options HCC; nevertheless, disturbance intestinal flora, immunotherapy shows low antitumor efficacy. An increasing body research indicates that particularly Akkermansia muciniphila ( A. ), vital treatment tumors. Studies have demonstrated diminished effectiveness in cancer patients associated with a reduction levels, suggesting levels significantly enhance efficacy immunotherapy. functions gut probiotic can treat prevent wide range illnesses, including cancer. Consequently, preserving abundance enough to lower danger developing disorders. In this review, we critically evaluate current on , focus biological properties functions. different illnesses treats were then discussed, way it works This review aims give novel plan well theoretical foundation improving

Language: Английский

Citations

1
A Comprehensive Review of Traditional Chinese Medicine in the Management of Ulcerative Colitis DOI

Hua-te Xu,

Jinhui Zhu, Xiangyun Lin

et al.

The American Journal of Chinese Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 39

Published: March 11, 2025

Ulcerative colitis (UC) is a chronic, nonspecific inflammatory disorder characterized by symptoms such as abdominal pain, diarrhea, hematochezia, and urgency during defecation. While the primary site of involvement colon, UC can extend to encompass entire rectum colon. The causes development mechanisms are still not well understood; nonetheless, it currently held that factors including environmental influences, genetic predispositions, intestinal mucosal integrity, gut microbiota composition, immune dysregulation contribute its development. Dysregulated responses pivotal in pathophysiology UC, these aberrant considered key contributors disease onset. In patients with cells become hyperactive erroneously target normal tissue, resulting cascades damage mucosa. therapeutic strategies employed for include immunosuppressive agents aminosalicylates corticosteroids. However, treatments often prove costly carry significant adverse effects — imposing considerable burden on patients. Traditional Chinese Medicine (TCM) has attracted worldwide attention because multi-target approach, minimal side effects, cost-effectiveness, favorable efficacy profiles. this review, ways which TCM modulates treatment ulcerative have been outlined. Research into modalities modulating pathways yielded promising advancements, individual herbs, herbal formulations, their derivatives, summarized. utilized treat system plays role regulating homeostasis. It imperative facilitate large-scale evidence-based medical research promote clinical application management UC.

Language: Английский

Citations

0
Ginsenosides Modulate Immunity via TLR4/MyD88/NF-κB Pathway and Gut Microbiota DOI
Ying Li, Meng Zhang, Kaiyue Zhang

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 142, P. 156763 - 156763

Published: April 12, 2025

Language: Английский

Citations

0
The Role of Gut Microbiota on Intestinal Fibrosis in Inflammatory Bowel Disease and Traditional Chinese Medicine Intervention DOI Creative Commons

Leyao Fang,

Hui Peng, Zhoujin Tan

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 5951 - 5967

Published: May 1, 2025

Inflammatory bowel disease (IBD) is a chronic, relapsing inflammatory disorder of the intestine, frequently complicated by intestinal fibrosis. As fibrosis progresses, it can result in luminal stricture and compromised function, significantly diminishing patients' quality life. Emerging evidence suggests that gut microbiota their metabolites contribute to pathogenesis IBD-associated influencing inflammation modulating immune responses. This review systematically explores mechanistic link between IBD evaluates therapeutic potential traditional Chinese medicine (TCM) interventions. Relevant studies were retrieved from PubMed, Web Science, Embase, Scopus, CNKI, Wanfang, VIP databases. Findings indicate TCM, including herbal prescriptions bioactive constituents, modulate composition microbial metabolites, ultimately alleviating through anti-inflammatory, immunemodulatory, anti-fibrotic mechanisms. These insights highlight TCM as promising strategy for targeting management

Language: Английский

Citations

0
Atractylenolide-1 Alleviates Ulcerative Colitis via Restraining RhoA/ROCK/MLC Pathway-Mediated Intestinal Barrier Dysfunction DOI

Zengxiang Gao,

Xuecheng Yu,

Wenlong Su

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

Using Atractylenolide-1 (AT-1) is a confident strategy for the treatment of ulcerative colitis (UC) due to its natural origin and notable pharmacological activity. The study investigated therapeutic effect AT-1 in dextran sodium sulfate (DSS)-induced mice Caco-2 cells while also exploring underlying molecular mechanisms. In this study, could reduce weight loss colon shortening significantly disease activity index (DAI), spleen index, histopathological scores UC mice. And was observed restore cell necrosis monolayer damage restored F-actin-mediated tight junction (TJ) protein redistribution alleviate mucosal injury cells. Moreover, regulated alanine, aspartic acid, glutamate metabolism; increased content related metabolites; promoted proliferation damaged mucous membranes results docking dynamics simulation showed that binding RhoA had stable conformation, it speculated main target AT-1. Further investigations revealed interference disrupted regulatory pathway Thus, inhibit reduction TJ proteins, alter DSS-mediated cytoskeletal migration, promote amino acid metabolism, subsequently permeability epithelium, thereby restoring barrier dysfunction features.

Language: Английский

Citations

0
Gut Commensal Barnesiella Intestinihominis Ameliorates Hyperglycemia and Liver Metabolic Disorders DOI Creative Commons
Ye Zhang, Dong Xu,

Xiaoxuan Cai

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract Recent studies have highlighted the role of gut microbiota in type 2 diabetes (T2D). Improving dysbiosis can be a potential strategy for prevention and management T2D. Here, this work finds that abundance Barnesiella intestinihominis is significantly decreased fecal T2D patients from 2‐independent centers. Oral treatment live B. (LBI) considerably ameliorates hyperglycemia liver metabolic disorders HFD/STZ‐induced models db/db mice. LBI‐derived acetate has similar protective effects against Mechanistically, enhances fibroblast growth factor 21 (FGF21) through inhibition histone deacetylase 9 (HDAC9) to increase H3K27 acetylation at FGF21 promoter. The screening puerarin Gegen Qinlian decoction microbiota‐dependent manner improved by promoting . This study suggests commensal puerarin, respectively as probiotic prebiotic

Language: Английский

Citations

3
PARP1 Exacerbates Prostatitis by Promoting M1 Macrophages Polarization through NF-κB Pathway DOI Creative Commons
Lu Jin, Jiaxing Chen, Jianhui Fu

et al.

Inflammation, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract PARP1 is recognized for its role as a DNA damage sensor and involvement in inflammatory diseases, but impact on prostatitis remains unclear. We aimed to elucidate how affects progression. Our results showed that 1% carrageenan-induced mouse model, Parp1 −/− prostatitic mice less pathological damage, decreased prostate weight, lower indices, macrophage neutrophil infiltration, down-regulated the expression of pro-inflammatory cytokines (IL-6, IL-12p70, CCL2, TNF) up-regulated anti-inflammatory cytokine IL-10 tissue. The NF-κB, TNF, IL-6 mRNA tissue decreased. In vitro experiments revealed M1(CD206 − CD86 +) LPS-induced decreased, did iNOS, NF-κB expression. Mechanically, treatment with inhibitor (AG14361) led significant reduction Phospho-NF-κB P65 protein macrophages. Following intervention inhibitors (Bay 11–7082), both levels were markedly diminished, meanwhile secretion IL-6, IL-10, IFN-γ, TNF exhibited pronounced dose-dependent decrease. Collectively, these findings indicated exacerbates by promoting M1 macrophages polarization via pathway, suggesting could be potential therapeutic target macrophage-based treatments prostatitis.

Language: Английский

Citations

0
Puerarin alleviates silicon dioxide-induced pulmonary inflammation and fibrosis via improving Autophagolysosomal dysfunction in alveolar macrophages of murine mice DOI

Wei Su,

Shuwen Gong, Yixin Luo

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 152, P. 114375 - 114375

Published: March 4, 2025

Language: Английский

Citations

0
Network pharmacology analysis of Lanatoside C: molecular targets and mechanisms in the treatment of ulcerative colitis DOI Creative Commons
Wenjing Zhu, Zhengjie Zhang, Xinyuan Wang

et al.

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: March 21, 2025

Introduction Ulcerative colitis (UC) is a chronic and progressive inflammatory disease of the intestines, marked by recurrent inflammation along digestive tract, leading to symptoms such as bloody diarrhea weight loss, severely impacting patients’ quality life. Despite extensive research, current therapeutic treatment for UC still faces challenges in long-term efficacy safety. Lanatoside C (LanC), type cardiac glycosides, has shown promising anti-inflammatory effects. This study employs network pharmacology investigate effects mechanisms LanC UC. Method LanC- UC-associated target genes datasets were retrieved from Genecards, DisGeNET, Gene Expression Omnibus database. Integration analysis identified common set potential targets treatment. Analyses ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) performed on these genes. Additionally, protein-protein interaction (PPI) was constructed identify top with highest connectivity. Molecular docking cellular experiments subsequently carried out further validated findings. Results 23 intersecting Among these, KDR, STAT3, ABCB1, CYP3A5, CYP2B6 emerged 5 high potential. Pathway indicated involvement fatty acid lipid metabolism, well xenobiotic metabolism pathways, which could be crucial LanC′s treating simulations revealed favorable binding between CYP2B6. Furthermore, In vitro demonstrated that significantly inhibits LPS-induced pro-inflammatory cytokines expression RAW264.7 cells. Conclusion demonstrates comprehensive overview elucidates its action. These findings offer theoretical basis optimizing clinical therapy underscore novel option

Language: Английский

Citations

0