Atractylenolide I Inhibits Nicotine-Induced Macrophage Pyroptosis and Alleviates Atherogenesis by Suppressing the TLR4/ROS/TXNIP/NLRP3 Pathway DOI Creative Commons
Huanhuan Li, Xian Liu, Yu‐Ping Wang

et al.

Metabolites, Journal Year: 2025, Volume and Issue: 15(5), P. 329 - 329

Published: May 15, 2025

Background/Objectives: Studies have shown that Atractylenolide I (AT-I) can exert anti-inflammatory and anti-oxidative effects, protecting against the development of various kinds cardiovascular diseases. However, whether AT-I prevents nicotine-induced atherogenesis is unknown. This study was designed to explore effects on macrophage pyroptosis progression atherosclerosis. Methods: RT-qPCR Western blot were used detect mRNA protein levels TXNIP pyroptosis-related factors in THP-1-derived macrophages. ELISA secretion pro-inflammatory cytokines. Hoechst/PI double-staining assay assess plasma membrane integrity. The ROS kit, LDH release caspase-1 activity kit production, release, activity. Oil Red O, HE, Masson staining evaluate lipid accumulation, lesion size, plaque stability HFD-fed apoE-/- mice. Results: treatment significantly decreased expression, disrupted integrity, down-regulated cytokines secretion, thereby inhibiting In addition, production expression TLR4 TXNIP. Lentivirus overexpression or TXNIP, pre-treatment with agonist, mainly reversed anti-pyroptotic nicotine-treated Additionally, administering mice markedly up-regulation proteins aortas. Enzymatic methods suggested improved dyslipidemia inflammation vivo. showed alleviated increased stability. Conclusions: Taken together, be regarded as a potential phytomedicine protects atherosclerosis triggered by nicotine.

Language: Английский

Deciphering Oxidative Stress in Cardiovascular Disease Progression: A Blueprint for Mechanistic Understanding and Therapeutic Innovation DOI Creative Commons
Z. Zhang, Jiawei Guo

Antioxidants, Journal Year: 2024, Volume and Issue: 14(1), P. 38 - 38

Published: Dec. 31, 2024

Oxidative stress plays a pivotal role in the pathogenesis and progression of cardiovascular diseases (CVDs). This review focuses on signaling pathways oxidative during development CVDs, delving into molecular regulatory networks underlying various disease stages, particularly apoptosis, inflammation, fibrosis, metabolic imbalance. By examining dual roles influences sex differences levels susceptibility, this study offers comprehensive understanding diseases. The integrates key findings from current research three ways. First, it outlines major CVDs associated with their respective pathways, emphasizing stress’s central pathology. Second, summarizes protective effects, mechanisms action, animal models antioxidants, offering insights future drug development. Third, discusses applications, advantages, limitations, potential targets gene therapy providing foundation for novel therapeutic strategies. These tables underscore systematic integrative nature while theoretical basis precision treatment CVDs. A contribution is differential effects across different stages addition to proposal innovative, multi-level intervention strategies, which open new avenues system.

Language: Английский

Citations

4
Herb-Derived Compounds from Radix Salviae Decoction (RSD) Modulated Cell Death of Vascular Smooth Muscle Cells DOI
Peizhong Liu, Qingqing Liu,

Guofu Zhong

et al.

European Journal of Integrative Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 102447 - 102447

Published: March 1, 2025

Language: Английский

Citations

0
M2 macrophages-derived exosomes for osteonecrosis of femoral head treatment: modulating neutrophil extracellular traps formation and endothelial phenotype transition DOI Creative Commons
Guanzhi Liu,

Ruomu Cao,

Qimeng Liu

et al.

Bone Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 1, 2025

Abstract Exosomes have shown good potential in ischemic injury disease treatments. However, evidence about their effect and molecular mechanisms osteonecrosis of femoral head (ONFH) treatment is still limited. Here, we revealed the cell biology characters ONFH area bone tissue single scale thus identified a novel approach based on M2 macrophages-derived exosomes (M2-Exos). We further show that M2-Exos are highly effective by modulating phenotypes communication between neutrophil endothelium including extracellular traps formation endothelial phenotype transition. Additionally, M2-Exos’ therapeutic attributed to high content miR-93-5p constructed overexpression model vitro vivo lentivirus adeno-associated virus respectively. Then found can not only reduce but also improve angiogenic ability cells. These results provided new theoretical basis for clinical application exosomes.

Language: Английский

Citations

0
Stem-Cell Derived Exosomal microRNAs as Biomarkers and Therapeutics for Pediatric Cardiovascular Disease DOI Creative Commons
Aaron H. Wasserman,

Bana Abolibdeh,

Reema Hamdan

et al.

Current Treatment Options in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 27(1)

Published: April 9, 2025

In recent years, several pre-clinical studies have demonstrated the therapeutic potential of stem cell-derived exosomes in treatment cardiovascular disease (CVD). Here, we evaluate their as biomarkers for detection and monitoring CVD, with a particular focus on pediatric heart disease. Exosomes isolated from cell sources, including mesenchymal cells (MSCs) pluripotent (PSCs), benefit function, inflammatory responses, angiogenesis injured diseased hearts. These carry variety cargo, such proteins, lipids, nucleic acids. However, majority contain non-coding RNA molecules. Review existing literature RNAs relationship to CVD suggests that containing microRNAs (miRNAs) can serve promising due presence circulation, ease isolation, potential. are especially screening diagnostic tools early congenital

Language: Английский

Citations

0
Critical analysis of descriptive microRNA data in the translational research on cardioprotection and cardiac repair: lost in the complexity of bioinformatics DOI Creative Commons
Mariann Gyöngyösi,

Julia Guthrie,

Ena Hašimbegović

et al.

Basic Research in Cardiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Language: Английский

Citations

0
Targeting Redox Signaling Through Exosomal MicroRNA: Insights into Tumor Microenvironment and Precision Oncology DOI Creative Commons
Moon Nyeo Park,

Myoungchan Kim,

Soojin Lee

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(5), P. 501 - 501

Published: April 22, 2025

Reactive oxygen species (ROS) play a dual role in cancer progression, acting as both signaling molecules and drivers of oxidative damage. Emerging evidence highlights the intricate interplay between ROS, microRNAs (miRNAs), exosomes within tumor microenvironment (TME), forming regulatory axis that modulates immune responses, angiogenesis, therapeutic resistance. In particular, stress not only stimulates exosome biogenesis but also influences selective packaging redox-sensitive miRNAs (miR-21, miR-155, miR-210) via RNA-binding proteins such hnRNPA2B1 SYNCRIP. These miRNAs, delivered through exosomes, alter gene expression recipient cells promote tumor-supportive phenotypes M2 macrophage polarization, CD8+ T-cell suppression, endothelial remodeling. This review systematically explores how this ROS–miRNA–exosome orchestrates communication across stromal cell populations under hypoxic inflammatory conditions. Particular emphasis is placed on NADPH oxidases, hypoxia-inducible factors, autophagy-related mechanisms regulating exosomal output. addition, we analyze relevance natural products herbal compounds—such curcumin, resveratrol, ginsenosides—which have demonstrated promising capabilities to modulate ROS levels, miRNA expression, dynamics. We further discuss clinical potential leveraging for therapy, including strategies involving mesenchymal stem cell-derived ferroptosis regulation, miRNA-based modulation. Incorporating insights from spatial transcriptomics single-cell analysis, provides mechanistic foundation development exosome-centered, redox-modulating therapeutics. Ultimately, work aims guide future research drug discovery efforts toward integrating medicine redox biology fight against cancer.

Language: Английский

Citations

0
Atractylenolide I Inhibits Nicotine-Induced Macrophage Pyroptosis and Alleviates Atherogenesis by Suppressing the TLR4/ROS/TXNIP/NLRP3 Pathway DOI Creative Commons
Huanhuan Li, Xian Liu, Yu‐Ping Wang

et al.

Metabolites, Journal Year: 2025, Volume and Issue: 15(5), P. 329 - 329

Published: May 15, 2025

Background/Objectives: Studies have shown that Atractylenolide I (AT-I) can exert anti-inflammatory and anti-oxidative effects, protecting against the development of various kinds cardiovascular diseases. However, whether AT-I prevents nicotine-induced atherogenesis is unknown. This study was designed to explore effects on macrophage pyroptosis progression atherosclerosis. Methods: RT-qPCR Western blot were used detect mRNA protein levels TXNIP pyroptosis-related factors in THP-1-derived macrophages. ELISA secretion pro-inflammatory cytokines. Hoechst/PI double-staining assay assess plasma membrane integrity. The ROS kit, LDH release caspase-1 activity kit production, release, activity. Oil Red O, HE, Masson staining evaluate lipid accumulation, lesion size, plaque stability HFD-fed apoE-/- mice. Results: treatment significantly decreased expression, disrupted integrity, down-regulated cytokines secretion, thereby inhibiting In addition, production expression TLR4 TXNIP. Lentivirus overexpression or TXNIP, pre-treatment with agonist, mainly reversed anti-pyroptotic nicotine-treated Additionally, administering mice markedly up-regulation proteins aortas. Enzymatic methods suggested improved dyslipidemia inflammation vivo. showed alleviated increased stability. Conclusions: Taken together, be regarded as a potential phytomedicine protects atherosclerosis triggered by nicotine.

Language: Английский

Citations

0