Naoqing formula alleviates cerebral ischemia/reperfusion injury induced inflammatory injury by regulating Csf3 mediated JAK/STAT pathway and macrophage polarization DOI Creative Commons

Y Ye,

Zhuqing Rao, Xin Xie

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 140, P. 156626 - 156626

Published: March 9, 2025

Upon cerebral ischemia/reperfusion injury (CIRI), the brain tissue experiences excessive inflammatory responses, which fuel activation of immune cells, thereby intensifying cellular damage and reactions. Naoqing formula (NQ), a traditional Chinese medicinal compound formulated with musk as primary component, has been extensively utilized in China for clinical treatment ischaemic stroke (IS). The precise pharmacological mechanism underlying NQ's efficacy managing IS remains elusive. In this study, we investigate protective effect molecular NQ against CIRI. C57BL/6 mice were to effects (130, 260 520mg/kg) middle artery occlusion (MCAO) induced CIRI mechanism. Employing biology techniques, transcriptomics, proteomics, network analyses, study assessed role response neuronal cells by establishing model cell microglia oxygen-glucose deprivation/reperfusion (OGD/R) lipopolysaccharide (LPS) stimulation. demonstrated significant mitigating infarction CIRI, achieved through enhancement cortical blood flow. Transcriptomic analyses revealed that mitigated caused modulating Csf3-mediated JAK/STAT pathway. Proteomic analysis further corroborated finding, indicating reduced impact regulating macrophage polarization. Notably, treated NQ, there was notable downregulation Csf3, JAK2, STAT3, STAT6, along co-localization Csf3 CD206. These observations suggested inhibited pathway exerted its anti-inflammatory orchestrating transition macrophages from M1 phenotype M2 phenotype, triggered Csf3. Consistent vivo findings, also microglial polarization vitro, protecting OGD/R- LPS-induced injury. This confirmed prevented inhibiting provided new perspective on use IS.

Language: Английский

Naoqing formula alleviates cerebral ischemia/reperfusion injury induced inflammatory injury by regulating Csf3 mediated JAK/STAT pathway and macrophage polarization DOI Creative Commons

Y Ye,

Zhuqing Rao, Xin Xie

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 140, P. 156626 - 156626

Published: March 9, 2025

Upon cerebral ischemia/reperfusion injury (CIRI), the brain tissue experiences excessive inflammatory responses, which fuel activation of immune cells, thereby intensifying cellular damage and reactions. Naoqing formula (NQ), a traditional Chinese medicinal compound formulated with musk as primary component, has been extensively utilized in China for clinical treatment ischaemic stroke (IS). The precise pharmacological mechanism underlying NQ's efficacy managing IS remains elusive. In this study, we investigate protective effect molecular NQ against CIRI. C57BL/6 mice were to effects (130, 260 520mg/kg) middle artery occlusion (MCAO) induced CIRI mechanism. Employing biology techniques, transcriptomics, proteomics, network analyses, study assessed role response neuronal cells by establishing model cell microglia oxygen-glucose deprivation/reperfusion (OGD/R) lipopolysaccharide (LPS) stimulation. demonstrated significant mitigating infarction CIRI, achieved through enhancement cortical blood flow. Transcriptomic analyses revealed that mitigated caused modulating Csf3-mediated JAK/STAT pathway. Proteomic analysis further corroborated finding, indicating reduced impact regulating macrophage polarization. Notably, treated NQ, there was notable downregulation Csf3, JAK2, STAT3, STAT6, along co-localization Csf3 CD206. These observations suggested inhibited pathway exerted its anti-inflammatory orchestrating transition macrophages from M1 phenotype M2 phenotype, triggered Csf3. Consistent vivo findings, also microglial polarization vitro, protecting OGD/R- LPS-induced injury. This confirmed prevented inhibiting provided new perspective on use IS.

Language: Английский

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